Parole chiave: Antiproliferative, K562, Metal, Thymidine, Antineoplastic Agent, Palladium Complex, Phosphine Derivative, Platinum Derivative, Thymidine Derivative, Thymine Derivative, Article, Cancer Cell, Cell Growth, Cell Strain K 562, Controlled Study, Data Analysis, Dna Replication, Drug Activity, Fast Atom Bombardment Mass Spectrometry, Human, Human Cell, Infrared Spectroscopy, Nuclear Magnetic Resonance Spectroscopy, Polymerase Chain Reaction, X Ray Analysis, Base Sequence, Cell Proliferation, Crystallography, X-Ray, Dna Primers, K562 Cells,
*** IBB - CNR *** Dipartimento di Scienze Ambientali, Seconda Universita di Napoli, via Vivaldi 43, 81100 Caserta, Italy. ER-Gen Tech, Dipartimento di Biochimica e Biologia Molecolare, Università di Ferrara, via L. Borsari, 46, 44100 Ferrara, Italy Dipartimento delle Scienze Biologiche Università di Napoli Federico II, via Mezzocannone 16, 80134 Napoli, Italy
Wang, D., Lippard, S.J., (2005) Nat. Rev. Drug Discov., 4, pp. 307-32
Janik, M.B., Lippert, B., (1999) J. Bio. Inorg. Chem., 4, pp. 645-653
Balcarova, Z., Kasparkova, J., Zakovska, A., Novakova, O., Sivo, M.F., Natile, G., Brabec, V., (1998) Mol. Pharmacol., 53, pp. 846-855
Janik, M.B., Hegmans, A., Freisinger, E., Lippert, B., (1999) J. Bio. Inorg. Chem., 4, pp. 130-139
Okada, T., El-Mehasseb, I.M., Kodaka, M., Tomohiro, T., Okamoto, K., Okuno, H., (2001) J. Med. Chem., 44, pp. 4661-4667
Farrell, N., Kelland, L.R., Roberts, J.D., Van Beusichem, M., (1992) Cancer Res., 52, pp. 5065-5072
Oxidative addition reactions between [M(PPh(3))(4)] (M=Pt and Pd) and N1-methylthymine (t)/3',5'-di-O-acetylthymidine (T) were carried out to give [M(II)(PPh(3))(2)Cl t (or T)] complexes, in which the metal is coordinated to the N3 of the base. All complexes were characterized by spectroscopic analyses (IR, NMR) and Fast Atom Bombardment mass spectrometry (FAB-MS); X-ray data for the thymine complexes and elemental analysis for the thymidine complexes are reported. The antiproliferative activity of the complexes was tested on human chronic myelogenous leukaemia K562 cells. Arrested polymerase-chain reaction analysis was carried on to correlate antiproliferative activity and inhibition of DNA replication. All Pd and Pt complexes exhibit antiproliferative activity, Pd complexes resulting always more active than Pt complexes. Arrested PCR data are strongly in agreement with the effects on cell growth, suggesting that inhibition of the DNA replication by the synthesized compounds is the major basis for their in vitro antiproliferative activity.
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L * Combined HAT/EZH2 modulation leads to cancer-selective cell death(55 visite) Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646. Impact Factor:5.008 DettagliEsporta in BibTeXEsporta in EndNote
133 Records (121 escludendo Abstract e Conferenze). Impact factor totale: 524.935 (476.613 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 550.08 (495.265 escludendo Abstract e Conferenze).