Protein P2 from Haemophilus influenzae: The role of its surface exposed loops on the mitogen-activated protein kinase cascade(139 visite) Galdiero S, Capasso D, Vitiello M, D'Isanto M, Pedone C, Galdiero M
Infection And Immunity (ISSN: 0019-9567), 2003 May; 71(5): 2798-2809.
Tipo di articolo: Journal Article,
Impact factor: 3.875, Impact factor a 5 anni: 4.096
Parole chiave: Bacterial Protein, Mitogen Activated Protein Kinase, Outer Membrane Protein F, Porin, Raf Protein, Synthetic Peptide, Unclassified Drug, Amino Acid Sequence, Article, Bacterial Membrane, Enzyme Activation, Enzyme Mechanism, Haemophilus Influenzae, Haemophilus Influenzae Type B, Human, Human Cell, Klebsiella Pneumoniae, Nonhuman, Nucleotide Sequence, Priority Journal, Protein Analysis, Protein Domain, Protein Function, Protein Interaction, Protein Phoe, Protein Phosphorylation, Protein Tertiary Structure, Signal Transduction, Map Kinase Signaling System, Models, Molecular, Molecular Sequence Data, Protein Structure, Secondary, Sequence Alignment, Sequence Homology, U937 Cells,
Affiliazioni:
*** IBB - CNR *** Dipartimento di Chimica Biologica, Univ. Degli Studi Napoli Federico II, Ist. di Biostrutture e Bioimmagini, 80134 Naples, Italy Dipartimento di Patologia Generale, Facolta di Medicina e Chirurgia, Seconda Università di Napoli, 80138 Naples, Italy Dipto. di Medicina Sperimentale, Facolta di Medicina e Chirurgia, Seconda Università di Napoli, Via De Crecchio 7, 80138 Naples, Italy
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Neary, J. M., Yi, K., Karalus, R. J., Murphy, T. F., Antibodies to loop 6 of the P2 porin protein of nontypeable Haemophilus influenzae are bactericidal against multiple strains (2001) Infect. Immun., 69, pp. 773-778
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Tracey, K. J., Fong, Y., Hesse, D. G., Manogue, K. R., Lee, A. T., Kuo, G. C., Lowry, S. F., Cerami, A., Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia (1987) Nature, 330, pp. 662-664
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Willis, S. A., Nisen, P. D., Differential induction of the mitogen-activated protein kinase pathway by bacterial lipopolysaccharide in cultured monocytes and astrocytes (1996) Biochem. J., 313, pp. 519-524
Yi, K., Murphy, T. F., Mapping of a strain-specific bactericidal epitope to the surface-exposed loop 5 on the P2 porin protein of non-typeable Haemophilus influenzae (1994) Microb. Pathog., 17, pp. 277-282
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Ziegler, E. J., Protective antibody to endotoxin core: The emperor's new clothes? (1988) J. Infect. Dis., 158, pp. 286-290
The outer membrane of gram-negative bacteria contains several proteins, and some of these proteins, the porins, have numerous biological functions in the interaction with the host; porins are involved in the activation of signal transduction pathways and, in particular, in the activation of the Raf/MEK1-MEK2/ mitogen-activated protein kinase (MAPK) cascade. The P2 porin is the most abundant outer membrane protein of Haemophilus influenzae type b. A three-dimensional structural model for P2 was constructed based on the crystal structures of Klebsiella pneumoniae OmpK36 and Escherichia coli PhoE and OmpF. The protein was readily assembled into the beta-barrel fold characteristic of porins, despite the low sequence identity with the template proteins. The model provides information on the structural features of P2 and insights relevant for prediction of domains corresponding to surface-exposed loops, which could be involved in the activation of signal transduction pathways. To identify the role of surface-exposed loops, a set of synthetic peptides were synthesized according to the proposed model and were assayed for MEK1-MEK2/MAPK pathway activation. Our results show that synthetic peptides corresponding to surface loops of protein P2 are able to activate the MEK1-MEK2/MAPK pathways like the entire protein, while peptides modeled on internal 13 strands are unable to induce significant phosphorylation of the MEK1-MEK2/MAPK pathways. In particular, the peptides corresponding to loops L5 (Lys206 to Gly219), L613 (Ser239 to Lys253), and L7 (Thr280 to Lys287) activate, as the whole protein, essentially JNK and p38.
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L * Combined HAT/EZH2 modulation leads to cancer-selective cell death(78 visite) Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646. Impact Factor:5.008 DettagliEsporta in BibTeXEsporta in EndNote
Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(207 visite) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 DettagliEsporta in BibTeXEsporta in EndNote
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S * Fusion in Coq(186 visite) Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596. Impact Factor:0.415 DettagliEsporta in BibTeXEsporta in EndNote
764 Records (741 escludendo Abstract e Conferenze). Impact factor totale: 2980.198 (2901.623 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 3058.145 (2970.822 escludendo Abstract e Conferenze).