Oliveri V, Lanza V, Milardi D, Viale M, Maric I, Sgarlata C, Vecchio G

Amino- and chloro-8-hydroxyquinolines and their copper complexes as proteasome inhibitors and antiproliferative agents
METALLOMICS (ISSN: 1756-5901), 2017 Oct 18; 9(10): 1439-1446.

Dell'Acqua S, Bacchella C, Monzani E, Nicolis S, Di Natale G, Rizzarelli E, Casella L

Prion Peptides Are Extremely Sensitive to Copper Induced Oxidative Stress
INORG CHEM (ISSN: 0020-1669), 2017 Sep 18; 56(18): 11317-11325.

Grasso GI, Bellia F, Arena G, Satriano C, Vecchio G, Rizzarelli E

Multitarget trehalose-carnosine conjugates inhibit Aβ aggregation, tune copper(II) activity and decrease acrolein toxicity
EUR J MED CHEM (ISSN: 0223-5234), 2017; 135: 447-457.

Di Natale G, Turi I, Pappalardo G, Sóvágó I, Rizzarelli E

Cross-Talk Between the Octarepeat Domain and the Fifth Binding Site of Prion Protein Driven by the Interaction of Copper(II) with the N-terminus
CHEM-EUR J (ISSN: 0947-6539), 2015 Mar 2; 21(10): 4071-4084.

Benetti F, Biarnés X, Attanasio F, Giachin G, Rizzarelli E, Legname G

Structural determinants in prion protein folding and stability
J MOL BIOL (ISSN: 0022-2836), 2014 Nov 11; 426(22): 3796-3810.

La Mendola D, Rizzarelli E

Evolutionary implications of metal binding features in different species' prion protein: an inorganic point of view
Biomolecules (ISSN: 2218-273x), 2014 May 23; 4(2): 546-565.

Tabbì G, Giuffrida A, Bonomo RP

Determination Of Formal Redox Potentials In Aqueous Solution Of Copper(ii) Complexes With Ligands Having Nitrogen And Oxygen Donor Atoms And Comparisonwith Their Epr And Uv–vis Spectral Features
J Chem Res (ISSN: 1873-3344, 0162-0134, 0162-0134print), 2013 Nov; 128: 137-145.

De Simonea A, Stanzione F, Marasco D, Vitagliano L, Esposito L

The Intrinsic Stability Of The Human Prion Beta-Sheet Region Investigated By Molecular Dynamics
Journal Of Biomolecular Structure & Dynamics, 2013 May 1; 31(5): 441-452.

Di Natale G, Osz K, Kállay C, Pappalardo G, Sanna D, Impellizzeri G, Sóvágó I, Rizzarelli E

Affinity, speciation, and molecular features of copper(II) complexes with a prion tetraoctarepeat domain in aqueous solution: insights into old and new results
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2013 Mar 11; 19(11): 3751-3761.

Travaglia A, La Mendola D, Magrì A, Nicoletti VG, Pietropaolo A, Rizzarelli E

Copper, BDNF and Its N-terminal domain: inorganic features and biological perspectives
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2012 Dec 3; 18(49): 15618-15631.

Magrì A, D'Alessandro F, Di Stefano DA, Campagna T, Pappalardo G, Impellizzeri G, La Mendola D

Copper(ii) Coordination Properties Of The Integrin Ligand Sequence Phsrn And Its New Beta-Cyclodextrin Conjugates
Journal Of Inorganic Biochemistry, 2012 Sep; 113: 15-24.

Jószai V, Turi I, Kállay C, Pappalardo G, Di Natale G, Rizzarelli E, Sóvágó I

Mixed metal copper(II)-nickel(II) and copper(II)-zinc(II) complexes of multihistidine peptide fragments of human prion protein
J Chem Res (ISSN: 1873-3344, 0162-0134, 0162-0134print), 2012 Jul; 112: 17-24.

Kállay C, Dávid A, Timári S, Nagy EM, Sanna D, Garribba E, Micera G, De Bona P, Pappalardo G, Rizzarelli E, Sóvágó I

Copper(II) complexes of rat amylin fragments
Dalton Trans (ISSN: 1477-9234, 1477-9226, 1477-9234electronic), 2011 Oct 14; 40(38): 9711-9721.

Arena G, Fattorusso R, Grasso G, Grasso GI, Isernia C, Malgieri G, Milardi D, Rizzarelli E

Zinc(II) complexes of ubiquitin: speciation, affinity and binding features
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2011 Oct 4; 17(41): 11596-11603.

Grasso GI, Arena G, Bellia F, Maccarrone G, Parrinello M, Pietropaolo A, Vecchio G, Rizzarelli E

Intramolecular weak interactions in the thermodynamic stereoselectivity of copper(II) complexes with carnosine-trehalose conjugates
Chemistry (ISSN: 1521-3765, 0947-6539, 1521-3765electronic), 2011 Sep 16; 17(34): 9448-9455.

Benetti F, Biarnes X, Attanasio F, Rizzarelli E, Laio A, Legname G

Description and Characterization of a Highly Stable Enthalpic Intermediate State in Mouse Prion Protein Folding
Prion (ISSN: 1933-6896), 2011 Apr; 5: N/D-N/D.

Kállay C, Turi I, Timári S, Nagy Z, Sanna D, Pappalardo G, De Bona P, Rizzarelli E, Sóvágó I

The effect of point mutations on copper(II) complexes with peptide fragments encompassing the 106-114 region of human prion protein
Monatshefte Fur Chemie (ISSN: 0026-9247), 2011 Apr; 142(4): 411-419.

Vagliasindi LI, Arena G, Bonomo RP, Pappalardo G, Tabbì G

Copper complex species within a fragment of the N-terminal repeat region in opossum PrP protein
Dalton Trans (ISSN: 1477-9226, 1477-9234, 1477-9234electronic), 2011 Mar 21; 40(11): 2441-2450.

Bonaccorso C, Sgarlata C, Grasso G, Zito V, Sciotto D, Arena G

A gemini guest triggers the self-assembly of a calixarene capsule in water at neutral pH
Chem Commun (ISSN: 1359-7345, 1364-548x, 1364-548xelectronic), 2011; 47(21): 6117-6119.

La Mendola D, Magrì A, Vagliasindi LI, Hansson Ö, Bonomo RP, Rizzarelli E

Copper(II) complex formation with a linear peptide encompassing the putative cell binding site of angiogenin
Dalton Trans (ISSN: 1477-9234, 1477-9226, 1477-9234electronic), 2010 Nov 28; 39(44): 10678-10684.

Di Natale G, Pappalardo G, Milardi D, Sciacca MF, Attanasio F, La Mendola D, Rizzarelli E

Membrane interactions and conformational preferences of human and avian prion N-terminal tandem repeats: The role of copper(II) ions, pH, and membrane mimicking environments
J Phys Chem B (ISSN: 1520-6106, 1520-5207, 1520-5207electronic), 2010 Nov 4; 114(43): 13830-13838.

Józsa E, Osz K, Kállay C, de Bona P, Damante CA, Pappalardo G, Rizzarelli E, Sóvágó I

Nickel(ii) and mixed metal complexes of amyloid-β N-terminus
Dalton Trans (ISSN: 1477-9226, 1477-9234, 1477-9234electronic), 2010 Sep 14; 39(30): 7046-7053.

Turi I, Kállay C, Szikszai D, Pappalardo G, Di Natale G, De Bona P, Rizzarelli E, Sóvágó I

Nickel(II) complexes of the multihistidine peptide fragments of human prion protein
J Chem Res (ISSN: 1873-3344, 0162-0134, 0162-0134print), 2010 Sep; 104(8): 885-891.

Benetti F, Pastorino L, Attanasio F, Rizzarelli E, Legname G

Insights into Prion Protein Stability
Prion (ISSN: 1933-6896), 2010 Jul; 4(3): N/D-N/D.

La Mendola D, Magrì A, Campagna T, Campitiello MA, Raiola L, Isernia C, Hansson O, Bonomo RP, Rizzarelli E

A doppel alpha-helix peptide fragment mimics the copper(II) interactions with the whole protein
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2010 Jun 1; 16(21): 6212-6223.

Di Natale G, Pappalardo G, Milardi D, Sciacca MFM, Attanasio F, La Mendola D, Rizzarelli E

Different Interactions Of Human And Avian Prion Proteins N-Terminal Tandem Repeat Peptides And Their Copper (ii) Complexes With Model Membranes
Journal Of Chemical Physics, 2010; 114: 13830-13838.

Jozsa E, Hosz K, Kallay C, De Bona P, Damante CA, Pappalardo G, Rizzarelli E, Sovago I

Nickel (ii) And Mixed Metal Complexes Of Amyloid-B N-Terminus. Results Of Potentiometric And Spectroscopic Studies On The Binary Nickel (ii) And Mixed Metal Nickel (ii)-Copper (ii) And Nickel (ii)-Copper (ii)-Zinc (ii) Complexes Of Peptide Fragments
Dalton Trans (ISSN: 1477-9226, 1477-9234, 1477-9234electronic), 2010; 39(30): 7046-7053.

Merlino A, Vitagliano L, Howes BD, Verde C, Di Prisco G, Smulevich G, Sica F, Vergara A

Combined Crystallographic and Spectroscopic Analysis of Trematomus bernacchii Hemoglobin Highlights Analogies and Differences in the Peculiar Oxidation Pathway of Antarctic Fish Hemoglobins
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2009 Dec; 91(12): 1117-1125.

Palladino P, Ronga L, Benedetti E, Rossi F, Ragone R

Peptide Fragment Approach to Prion Misfolding: The Alpha-2 Domain
Int J Pept Res Ther (ISSN: 1573-3149, 0929-5666), 2009 Sep; 15(3): 165-176.

Pietropaolo A, Muccioli L, Zannoni C, Rizzarelli E

Conformational preferences of the full chicken prion protein in solution and its differences with respect to mammals
Chemphyschem (ISSN: 1439-7641, 1439-4235, 1439-7641electronic), 2009 Jul 13; 10(9-10): 1500-1510.

La Mendola D, Pietropaolo A, Pappalardo G, Zannoni C, Rizzarelli E

Prion Proteins Leading to Neurodegeneration (vol 5, pg 579, 2008)
Current Alzheimer Research (ISSN: 1567-2050), 2009 Jun; 6(3): N/D-N/D.

Di Natale G, Osz K, Nagy Z, Sanna D, Micera G, Pappalardo G, Sovago I, Rizzarelli E

The Interaction Of Copper (ii) With The Prion Peptide Fragment Huprp (76-114) Encompassing Four Histidyl Residues Within And Outside The Octarepeat Domain
Inorg Chem (ISSN: 0020-1669, 1520-510x, 1520-510xelectronic), 2009 May 4; 48(9): 4239-4250.

La Mendola D, Magrì A, Hansson O, Bonomo RP, Rizzarelli E

Copper(II) complexes with peptide fragments encompassing the sequence 122-130 of human doppel protein
Journal Of Inorganic Biochemistry, 2009 May; 103(5): 758-765.

Ronga L, Palladino P, Ragone R, Benedetti E, Rossi F

A thermodynamic approach to the conformational preferences of the 180-195 segment derived from the human prion protein α2-helix
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009; 15(1): 30-35.

La Mendola D, Pietropaolo A, Pappalardo G, Zannoni C, Rizzarelli E

Prion proteins leading to neurodegeneration
Current Alzheimer Research (ISSN: 1567-2050), 2008 Dec; 5(6): 579-590.

Bonomo RP, Pappalardo G, Rizzarelli E, Tabbì G, Vagliasindi LI

Studies of nitric oxide interaction with mono- and dinuclear copper(II) complexes of prion protein bis-octarepeat fragments
Dalton Trans (ISSN: 1477-9226, 1477-9234, 1477-9234electronic), 2008 Sep 7; (29): 3805-3816.

Pietropaolo A, Muccioli L, Zannoni C, La Mendola D, Maccarrone G, Pappalardo G, Rizzarelli E

Unveiling the role of histidine and tyrosine residues on the conformation of the avian prion hexarepeat domain
J Phys Chem B (ISSN: 1520-6106, 1520-5207, 1520-5207electronic), 2008 Apr 24; 112(16): 5182-5188.

De Simone A, Pedone C, Vitagliano L

Structure, dynamics, and stability of assemblies of the human prion fragment SNQNNF
Biochem Biophys Res Commun (ISSN: 1090-2104, 0006-291x, 1090-2104electronic), 2008 Feb 15; 366(3): 800-806.

Ronga L, Palladino P, Saviano G, Tancredi T, Benedetti E, Ragone R, Rossi F

Structural characterization of a neurotoxic threonine-rich peptide corresponding to the human prion protein α2-helical 180-195 segment and comparison with full-length α2-helix-derived peptides
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2008; 14(10): 1096-1102.

Bonomo RP, Pappalardo G, Rizzarelli E, Santoro AM, Tabbì G, Vagliasindi LI

Nitrogen oxide interaction with copper complexes formed by small peptides belonging to the prion protein octa-repeat region
Dalton Trans (ISSN: 1477-9226, 1477-9234, 1477-9234electronic), 2007 Apr 14; (14): 1400-1408.

Ronga L, Langella E, Palladino P, Marasco D, Tizzano B, Saviano M, Pedone C, Improta R, Ruvo M

Does Tetracycline Bind Helix 2 Of Prion? An Integrated Spectroscopical And Computational Study Of The Interaction Between The Antibiotic And Alpha Helix 2 Human Prion Protein Fragments
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2007 Feb 15; 66(3): 707-715.

Ronga L, Palladino P, Costantini S, Facchiano A, Ruvo M, Benedetti E, Ragone R, Rossi F

Conformational diseases and structure-toxicity relationships: Lessons from prion-derived peptides
Curr Protein Pept Sci (ISSN: 1389-2037), 2007 Feb; 8(1): 83-90.

Rao M, Russo F, Granata V, Berisio R, Zagari A, Gianfreda L

Fate of prions in soil: Interaction of a recombinant ovine prion protein with synthetic humic-like mineral complexes
Soil Biol Biochem (ISSN: 0038-0717), 2007 Feb; 39(2): 493-504.

Osz K, Nagy Z, Pappalardo G, Di Natale G, Sanna D, Micera G, Rizzarelli E, Sóvágó I

Copper(II) interaction with prion peptide fragments encompassing histidine residues within and outside the octarepeat domain: speciation, stability constants and binding details
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2007; 13(25): 7129-7143.

Pappalardo M, Milardi D, Grasso D, La Rosa C

Steered molecular dynamics studies reveal different unfolding pathways of prions from mammalian and non-mammalian species
New J Chem (ISSN: 1144-0546), 2007; 31(6): 901-905.

Ronga L, Palladino P, Tizzano B, Marasco D, Benedetti E, Ragone R, Rossi F

Effect of salts on the structural behavior of hPrP α2-helix-derived analogues: The counterion perspective
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2006 Dec; 12(12): 790-795.

Mazzarella L, Vergara A, Vitagliano L, Merlino A, Bonomi G, Scala S, Verde C, Di Prisco G

High resolution crystal structure of deoxy hemoglobin from Trematomus bernacchii at different pH values: The role of histidine residues in modulating the strength of the root effect
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2006 Nov 1; 65(2): 490-498.

Esposito L, Pedone C, Vitagliano L

Molecular dynamics analyses of cross-beta-spine steric zipper models: beta-sheet twisting and aggregation
Proc Natl Acad Sci U S A (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2006 Sep 1; 103(31): 11533-11538.

Ronga L, Tizzano B, Palladino P, Ragone R, Urso E, Maffia M, Ruvo M, Benedetti E, Rossi F

The prion protein: structural features and related toxic peptides
Chem Biol Drug Des Chemical Biology And Drug Design (ISSN: 1747-0277, 1747-0285), 2006 Sep; 68(3): 139-147.

Joszal V, Nagy Z, Osz K, Sanna D, Di Natale G, La Mendola D, Pappalardo G, Rizzarelli E, Sovago I

Transition metal complexes of terminally protected peptides containing histidyl residues
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2006 Sep; 100(8): 1399-1409.

Langella E, Improta R, Crescenzi O, Barone V

Assessing the acid-base and conformational properties of histidine residues in human prion protein (125-228) by means of pKα calculations and molecular dynamics simulations
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2006 Jul 1; 64(1): 167-177.

Granata V, Palladino P, Tizzano B, Negro A, Berisio R, Zagari A

The effect of the osmolyte trimethylamine N-oxide on the stability of the prion protein at low pH
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2006 Jun 15; 82(3): 234-240.

Mazzarella L, Bonomi G, Lubrano MC, Merlino A, Riccio A, Vergara A, Vitagliano L, Verde C, Di Prisco G

Minimal structural requirements for root effect: Crystal structure of the cathodic hemoglobin isolated from the Antarctic fish Trematomus newnesi
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2006 Feb 1; 62(2): 316-321.

Palladino P, Ronga L, Tizzano B, Rossi F, Ragone R, Tancredi T, Saviano G, Facchiano A, Costantini S, Ruvo M, Benedetti E

Prion protein misfolding: Conformational stability of the alpha 2-helix
Understanding Biology Using Peptides, 2006; N/D: N/D-N/D.

Grasso D, Grasso G, Guantieri V, Impellizzeri G, La Rosa C, Milardi D, Micera G, Osz K, Pappalardo G, Rizzarelli E, Sanna D, Sovago I

Environmental effects on a prion's helix II domain: copper(II) and membrane interactions with PrP180-193 and its analogues
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2005 Dec 23; 12(2): 537-547.

La Mendola D, Bonomo RP, Impellizzeri G, Maccarrone G, Pappalardo G, Pietropaolo A, Rizzarelli E, Zito V

Copper(II) complexes with chicken prion repeats: Influence of proline and tyrosine residues on the coordination features
J Biol Inorg Chem (ISSN: 0949-8257, 1432-1327electronic, 0949-8257print), 2005 Sep; 10(5): 463-475.

Tizzano B, Palladino P, De Capua A, Marasco D, Rossi F, Benedetti E, Pedone C, Ragone R, Ruvo M

The Human Prion Protein Alpha2 Helix: A Thermodynamic Study Of Its Conformational Preferences
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2005 Apr 1; 59(1): 72-79.

Di Natale G, Impellizzeri G, Pappalardo G

Conformational properties of peptide fragments homologous to the 106-114 and 106-126 residues of the human prion protein: a CD and NMR spectroscopic study
Org Biomol Chem (ISSN: 1477-0520, 1477-0539, 1477-0539electronic), 2005 Feb 7; 3(3): 490-497.

Bonomo RP, Cucinotta V, Giuffrida A, Impellizzeri G, Magrì A, Pappalardo G, Rizzarelli E, Santoro AM, Tabbì G, Vagliasindi LI

A re-investigation of copper coordination in the octa-repeats region of the prion protein
Dalton Trans (ISSN: 1477-9226, 1477-9234, 1477-9234electronic), 2005 Jan 7; (1): 150-158.

Langella E, Improta R, Barone V

Checking the pH-induced conformational transition of prion protein by molecular dynamics simulations: Effect of protonation of histidine residues
Biophysical Journal (ISSN: 0006-3495, 1542-0086), 2004 Dec; 87(6): 3623-3632.

Pappalardo M, Milardi D, La Rosa C, Zannoni C, Rizzarelli E, Grasso D

A molecular dynamics study on the conformational stability of PrP 180-193 helix II prion fragment
Chemical Physics Letters (ISSN: 0009-2614), 2004 Jun 1; 390(4-6): 511-516.

Grasso D, Milardi D, La Rosa C, Rizzarelli E

The different role of Cu++ and Zn++ ions in affecting the interaction of prion peptide PrP106-126 with model membranes
Chem Commun (ISSN: 1359-7345, 1364-548x, 1364-548xelectronic), 2004 Jan 21; 10(2): 246-247.

Bonomo RP, Castronovo BM, Santoro AM

A Spectroscopic Investigation Of The Interaction Between Nitrogen Monoxide And Copper Sites Of The Fungal Laccase From Rigidoporus Lignosus
Dalton Trans, 2004 Jan 7; N/D: 104-112.

Brown DR, Guantieri V, Grasso G, Impellizzeri G, Pappalardo G, Rizzarelli E

Copper(II) complexes of peptide fragments of the prion protein. Conformation changes induced by copper(II) and the binding motif in C-terminal protein region
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2004 Jan; 98(1): 133-143.

Barone V, Improta R, Rega N

Computation of protein pK's values by an integrated density functional theory/Polarizable Continuum Model approach
Theor Chem Acc (ISSN: 1432-881x), 2004; 111(2-6): 237-245.

Tizzano B, Marasco D, Benedetti E, De Capua A, Palladino P, Pedone C, Perretta G, Rossi F, Ragone R, Ruvo M

Conformational conversion of prion proteins: Role synthetic PRP 173-195 fibrillogenic peptide
Peptide Revolution, 2004; N/D: N/D-N/D.

Pappalardo G, Impellizzeri G, Campagna T

Copper(II) binding of prion protein's octarepeat model peptides
Inorg Chim Acta (ISSN: 0020-1693), 2004; 357(1): 185-194.

La Mendola D, Bonomo R, Maccarrone G, Pappalardo G, Rizzarelli E

A thermodynamic and spectroscopic study on the copper(II) complexes with hexarepeats fragments of the avian prion protein
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2003 Jul 15; 96(1): N/D-N/D.

Bonomo RP, Grasso D, Grasso G, Guantieri V, Impellizzeri G, La Rosa C, Milardi D, Pappalardo G, Tabbì G, Rizzarelli E

Metal binding to prion protein
Metal-Ligand Interactions - Molecular Nano- Micro- And Macro-Systems In Complex Environments, 2003 Jan 01; 116: 21-39.

Grasso D, Milardi D, Guantieri V, La Rosa C, Rizzarelli E

Interaction of prion peptide PrP 180-193 with DPPC model membranes: A thermodynamic study
New J Chem (ISSN: 1144-0546), 2003; 27(2): 359-364.

Menziani MC, De Benedetti PG, Langella E, Barone V

Seeking for binding determinants of the prion protein to human plasminogen
Mol Phys (ISSN: 0026-8976), 2003; 101(17): 2763-2773.

Grasso D, Milardi D, La Rosa C, Rizzarelli E

DSC study of the interaction of the prion peptide PrP106-126 with artificial membranes
New J Chem (ISSN: 1144-0546), 2001; 25(12): 1543-1548.

Bonomo RP, Imperllizzeri G, Pappalardo G, Rizzarelli E, Tabbì G

Copper(II) binding modes in the prion octapeptide PHGGGWGQ: A spectroscopic and voltammetric study
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2000 Nov 17; 6(22): 4195-4202.

Campagna T, Grasso G, Rizzarelli E, Vecchio G

L- and D-cysteine derivatives of β-cyclodextrin: Different molecular recognition properties of their copper(II) complexes for amino acids
Inorg Chim Acta (ISSN: 0020-1693), 1998; 275-276: 395-400.

Marletta F, Rizzarelli E, Mangiameli A, Brogna A, Sammartano S, Monaco S, Blasi A

Effects of graded doses of copper complexes of L-aminoacids on gastric acid secretion in rats
Boll Soc Ital Biol Sper (ISSN: 0037-8771), 1979 Apr 30; 55(8): 769-775.