Keywords Parole chiave: Azurin, Binding Protein, Copper Ion, Cyclopeptide, Mercury, Metal Ion, Plastocyanin, Alcaligenes, Article, Binding Affinity, Circular Dichroism, Nonhuman, Peptide Synthesis, Protein Binding, Protein Structure, Ultraviolet Spectroscopy, X Ray Analysis, Amino Acid Sequence, Binding Sites, Drug Design, Kinetics, Metalloproteins, Molecular Sequence Data, Spectrophotometry, Achromobacter Denitrificans, Populus Berolinensis, Chemical Synthesis, Metabolism, Chemistry, Methods,
Affiliations Affiliazioni: Department of Chemistry, Research Ctr. on Bioactive Peptides, University of Naples Federico II, via Mezzocannone 4, 80134 Napoli, Italy
References Riferimenti: Not available. Non disponibili.
Design of metal ion binding peptides
Two cyclic and branched peptides (PLA and AZU) were synthesized with the aim of reproducing the active site of the blue copper proteins plastocyanin and azurin. Both peptides, designed on the basis of the x-ray structures of Poplar plastocyanin and Alcaligenes denitrificans azurin, contain the same coordinating residues of the parent native proteins. The visible spectra of PLA in the presence of equimolar amount of Cu(II) strongly support the interaction between the peptide and copper(II) ion. The CD titration of AZU with the Hg(II) ion indicates for the formation of two species, [AZUHg]+ and [AZUHg2]3+ having binding constants (Keq) of 3.10(6) and 2.10(4) M-1, respectively.
Vitiello M, Finamore E, Falanga A, Raieta K, Cantisani M, Galdiero F, Pedone C, Galdiero M, Galdiero S * Fusion in Coq(392 visite) Lecture Notes In Computer Science (ISSN: 0302-9743, 0302-974335404636319783540463634, 0302-974335402975459783540297543), 2001; 2178LNCS: 583-596. Impact Factor:0.415 DettagliEsporta in BibTeXEsporta in EndNote
239 Records (227 escludendo Abstract e Conferenze). Impact factor totale: 1054.831 (1013.765 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 1100.943 (1054.765 escludendo Abstract e Conferenze).
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