Parole chiave: Cyclolinopeptide A, Cyclopeptide, Cyclophilin, Cyclosporin A, Absorption Spectroscopy, Article, Circular Dichroism, Enzyme Activity, Fluorescence Spectroscopy, Protein Protein Interaction, Protein Structure, Amino Acid Isomerases, Animal, Carrier Proteins, Cattle, Chromatography, Affinity, Immunosuppression, Kinetics, Peptide Fragments, Peptidylprolyl Isomerase, Protein Binding, Protein Conformation, Spectrometry, Spectrophotometry, Thymus Gland, Bovinae,
CIRPB and CEINGE, Biotecnologie Avanzate, via Mezzocannone 4, 80134 Napoli, Italy
Cyclophilin A, a peptidyl-prolyl cis-trans isomerase that catalyzes the otherwise slow isomerization of Xaa-Pro imidic bond, specifically, binds the immunosuppressant cyclosporin A. Herein we repel evidence on binding of cyclolinopeptide A and its synthetic analogue, [Aib(5,6)-D-Ala(8)] cyclolinopeptide, to bovine cyclophilin A. Binding experiments were monitored by fluorescence, CD, and second-derivative spectroscopies, evidencing no remarkable rearrangement of protein structure organization. The possibility, that cyclolinopeptide A could act as a substitute of cyclosporin A in the immunosuppression modulation is also briefly discussed. (C) 1995 John Wiley and Sons, Inc.
398 Records (382 escludendo Abstract e Conferenze). Impact factor totale: 1543.1 (1480.983 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 1611.262 (1542.453 escludendo Abstract e Conferenze).