Two alternative mRNAs coding for the angiogenic factor, placenta growth factor (PlGF), are transcribed from a single gene of chromosome 14(307 visite) Maglione D, Guerriero V, Viglietto G, Ferraro MG, Aprelikova O, Alitalo K, Del Vecchio S, Lei KJ, Chou JY, Persico MG
Parole chiave: Gallus Gallus, Angiogenic Factor, Placental Growth Factor, Unclassified Drug, Alternative Rna Splicing, Article, Choriocarcinoma, Chromosome 14, Genetic Conservation, Genetic Transcription, Human, Human Cell, Priority Journal, Alternative Splicing, Amino Acid Sequence, Animal, Base Sequence, Pair 14, Cloning, Molecular, Conserved Sequence, Gene Expression, Structural, Growth Substances, Introns, Molecular Sequence Data, Neovascularization, Pathologic, Oligodeoxyribonucleotides, Pregnancy Proteins, Messenger, Species Specificity, Support, Non-U.S. Gov, Non-U. S. Gov,
Intl. Inst. of Genet. and Biophysics, CNR, Via G. Marconi, 12, 80125 Naples, Italy SIFI SpA, 95020 Lavinaio (CT), Italy Departments of Pathology, University of Helsinki, SF-00290 Helsinki, Finland Fondazione G. Pascale, Ist. Naz. Stud. e la Cura dei Tumori, 80131 Naples, Italy Laboratory of Human Genetics, Natl. Inst. Child Hlth. Hum. Devmt., National Institutes of Health, Bethesda, MD 20892, United States
We have previously reported on the identification of a cDNA (placenta growth factor, PlGF) coding for a novel angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF). Biochemical and functional characterization of PlGF derived from transfected COS-1 cells revealed that it is a glycosylated dimeric secreted protein able to stimulate endothelial cell growth in vitro. Here, we report the isolation and characterization of the PlGF gene located on chromosome 14. At least two different mRNAs are produced from this single-copy gene in different cell lines and tissues. Sequence comparison of the polypeptides encoded by the two different isolated cDNAs indicates that they are identical except for the insertion of a highly basic 21 amino acid stretch at the carboxyl end of the protein. RNA expression analysis of several tissues, tumors and cell lines indicates differential distribution of the two PlGF mRNAs. Finally, preliminary results indicate that the PlGF gene has been conserved in evolution, since the human PlGF cDNA hybridizes to sequences present in the genomic DNA of Drosophila, Xenopus, chicken and mouse.
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L * Combined HAT/EZH2 modulation leads to cancer-selective cell death(134 visite) Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646. Impact Factor:5.008 DettagliEsporta in BibTeXEsporta in EndNote
503 Records (487 escludendo Abstract e Conferenze). Impact factor totale: 2033.947 (1973.877 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 2063.289 (2000.601 escludendo Abstract e Conferenze).