Radioiodide induces apoptosis in human thyroid tissue in culture(141 visite) Russo E, Guerra A, Marotta V, Faggiano A, Colao A, Del Vecchio S, Tonacchera M, Vitale M
*** IBB - CNR *** Department of Cellular and Molecular Biology and Pathology, University Federico II, 80131 Naples, Italy Department of Medicine and Surgery, University of Salerno, Via Allende, 84081 Baronissi Salerno, Italy Department of Clinical Medicine and Surgery, University Federico II, 80131 Naples, Italy National Cancer Institute, Fondazione G. Pascale, Naples, Italy Department of Biomorphological and Functional Sciences, University of Naples Federico II, 80131 Naples, Italy Department of Endocrinology, Research Center of Excellence AmbiSEN, University of Pisa, 56124 Pisa, Italy
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Radioiodide ((131)I) is routinely used for the treatment of toxic adenoma, Graves' disease, and for ablation of thyroid remnant after thyroidectomy in patients with thyroid cancer. The toxic effects of ionizing radiations on living cells can be mediated by a necrotic and/or apoptotic process. The involvement of apoptosis in radiation-induced cell death in the thyrocytes has been questioned. The knowledge of the mechanisms that underlie the thyrocyte death in response to radiations can help to achieve a successful treatment with the lowest (131)I dose. We developed a method to study the effects of (131)I in human thyroid tissue in culture, by which we demonstrated that (131)I induces thyroid cell apoptosis. Human thyroid tissues of about 1 mm(3) were cultured in vitro and cell viability was determined up to 3 weeks by the MTT assay. Radioiodide added to the culture medium was actively taken up by the tissues. The occurrence of apoptosis in the thyrocytes was assessed by measuring the production of a caspase-cleavage fragment of cytokeratin 18 (M30) by an enzyme-linked immunoassay. Neither variation of cell number nor spontaneous apoptosis was revealed after 1 week of culture. (131)I added to the culture medium induced a dose-dependent and a time-dependent generation of M30 fragment. The apoptotic process was confirmed by the generation of caspase-3 and PARP cleavage products. These results demonstrate that (131)I induces apoptosis in human thyrocytes. Human thyroid tissue cultures may be useful to investigate the cell death pathways induced by (131)I.
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124 Records (108 escludendo Abstract e Conferenze). Impact factor totale: 574.86 (510.173 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 592.287 (519.671 escludendo Abstract e Conferenze).
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