Radioiodide induces apoptosis in human thyroid tissue in culture(156 visite) Russo E, Guerra A, Marotta V, Faggiano A, Colao A, Del Vecchio S, Tonacchera M, Vitale M
*** IBB - CNR *** Department of Cellular and Molecular Biology and Pathology, University Federico II, 80131 Naples, Italy Department of Medicine and Surgery, University of Salerno, Via Allende, 84081 Baronissi Salerno, Italy Department of Clinical Medicine and Surgery, University Federico II, 80131 Naples, Italy National Cancer Institute, Fondazione G. Pascale, Naples, Italy Department of Biomorphological and Functional Sciences, University of Naples Federico II, 80131 Naples, Italy Department of Endocrinology, Research Center of Excellence AmbiSEN, University of Pisa, 56124 Pisa, Italy
Riferimenti:
Bonnema, S.J., Hegedus, L., Radioiodine therapy in benign thyroid diseases: Effects, side effects, and factors affecting therapeutic outcome (2012) Endocrinol. Rev., , 10.1210/er.2012-103
Reiners, C., Hanscheid, H., Luster, M., Lassmann, M., Verburg, F.A., Radioiodine for remnant ablation and therapy of metastatic disease (2011) Nat. Rev. Endocrinol., 7 (10), pp. 589-595. , 10.1038/nrendo.2011.134 21826102 10.1038/nrendo.2011.134 1:CAS:528:DC%2BC3MXht1ajsrrK
Sawin, C.T., Becker, D.V., Radioiodine and the treatment of hyperthyroidism: The early history (1997) Thyroid, 7 (2), pp. 163-176. , 9133679 10.1089/thy.1997.7.163 1:STN:280:DyaK2s3nvFWksw%3D%3D
Wartofsky, L., Van Nostrand, D., Radioiodine treatment of well-differentiated thyroid cancer (2012) Endocrine, 42 (3), pp. 506-513. , 10.1007/s12020-012-9729-5 22733393 10.1007/s12020-012-9729-5 1:CAS:528:DC%2BC38XptFagsLk%3D
Bonnema, S.J., Fast, S., Hegedus, L., Non-surgical approach to the benign nodular goiter: New opportunities by recombinant human TSH-stimulated 131I-therapy (2012) Endocrine, 40 (3), pp. 344-353. , 10.1007/s12020-011-9542-6 10.1007/s12020-011-9542-6
Sawka, A.M., Thephamongkhol, K., Brouwers, M., Thabane, L., Browman, G., Gerstein, H.C., Clinical review 170: A systematic review and metaanalysis of the effectiveness of radioactive iodine remnant ablation for well-differentiated thyroid cancer (2004) J. Clin. Endocrinol. Metab., 89 (8), pp. 3668-3676. , 10.1210/jc.2003-03116789/8/3668 15292285 10.1210/jc.2003-031167 1:CAS:528:DC%2BD2cXmslKmsbc%3D
Vitale, M., Di Matola, T., D'Ascoli, F., Salzano, S., Bogazzi, F., Fenzi, G., Martino, E., Rossi, G., Iodide excess induces apoptosis in thyroid cells through a p53-independent mechanism involving oxidative stress (2000) Endocrinology, 141 (2), pp. 598-605. , 10650940 10.1210/en.141.2.598 1:CAS:528:DC%2BD3cXmvFCltg%3D%3D
Driessens, N., Versteyhe, S., Ghaddhab, C., Burniat, A., De Deken, X., Van Sande, J., Dumont, J.E., Corvilain, B., Hydrogen peroxide induces DNA single- and double-strand breaks in thyroid cells and is therefore a potential mutagen for this organ (2009) Endocrinol. Relat. Cancer, 16 (3), pp. 845-856. , 10.1677/ERC-09-0020 10.1677/ERC-09-0020 1:CAS:528:DC%2BD1MXht1GnurnK
Amundson, S.A., Bittner, M., Meltzer, P., Trent, J., Fornace, Jr.A.J., Induction of gene expression as a monitor of exposure to ionizing radiation (2001) Radiat. Res., 156 (5 PART 2), pp. 657-661. , 11604088 10.1667/0033-7587(2001)156[0657:IOGEAA]2.0.CO
2 1:CAS:528:DC%2BD3MXosV2ks78%3D
Turgut, B., Babul, A., Ozdemir, O., Erselcan, T., Evaluation of the cell death pathway and apoptosis-stunning effect relationship after low- and high-dose I-131 administrations in rat thyroid tissue (2006) Cancer Biother. Radiopharm., 21 (4), pp. 342-351. , 10.1089/cbr.2006.21.342 16999600 10.1089/cbr.2006.21.342 1:CAS:528:DC%2BD28XhtVSgu7jI
Yang, T., Namba, H., Hara, T., Takmura, N., Nagayama, Y., Fukata, S., Ishikawa, N., Yamashita, S., P53 induced by ionizing radiation mediates DNA end-jointing activity, but not apoptosis of thyroid cells (1997) Oncogene, 14 (13), pp. 1511-1519. , 10.1038/sj.onc.1200979 9129141 10.1038/sj.onc.1200979 1:CAS:528:DyaK2sXislKmsLs%3D
Marx, K., Moka, D., Schomacker, K., Fischer, T., Gabruk-Szostak, B., Kobe, C., Dietlein, M., Schicha, H., Cell death induced by 131-I in a differentiated thyroid carcinoma cell line in vitro: Necrosis or apoptosis? (2006) Nucl. Med. Commun., 27 (4), pp. 353-358. , 10.1097/01.mnm.0000199475.08407 16531921 10.1097/01.mnm.0000199475.08407. e2
Hershman, J.M., Okunyan, A., Rivina, Y., Cannon, S., Hogen, V., Prevention of DNA double-strand breaks induced by radioiodide-(131)I in FRTL-5 thyroid cells (2011) Endocrinology, 152 (3), pp. 1130-1135. , 10.1210/en.2010-1163 21190956 10.1210/en.2010-1163 1:CAS:528: DC%2BC3MXktVGrtro%3D
Marotta, V., Guerra, A., Zatelli, M.C., Uberti, E.D., Stasi, V.D., Faggiano, A., Colao, A., Vitale, M., BRAF mutation positive papillary thyroid carcinoma is less advanced when Hashimoto's thyroiditis lymphocytic infiltration is present (2013) Clin. Endocrinol. (Oxf), , In press. doi: 10.1111/cen.12194
Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J. Immunol. Methods, 65 (1-2), pp. 55-63. , 0022-1759(83)90303-4 6606682 10.1016/0022-1759(83)90303-4 1:STN:280:DyaL2c%2FovFSmtw%3D%3D
Young, B.A., Baker, T.G., The ultrastructure of rat thyroid glands under experimental conditions in organ culture (1982) J. Anat., 135 (PART 2), pp. 407-412. , 7174511 1:STN:280:DyaL3s%2Fot1Chtg%3D%3D
Illario, M., Cavallo, A.L., Monaco, S., Di Vito, E., Mueller, F., Marzano, L.A., Troncone, G., Vitale, M., Fibronectin-induced proliferation in thyroid cells is mediated by αvβ3 integrin through Ras/Raf-1/MEK/ERK and calcium/CaMKII signals (2005) J. Clin. Endocrinol. Metab., 90 (5), pp. 2865-2873. , 15687337 10.1210/jc.2004-1520 1:CAS:528:DC%2BD2MXkt1Wgt7c%3D
Illario, M., Amideo, V., Casamassima, A., Andreucci, M., Di Matola, T., Miele, C., Rossi, G., Vitale, M., Integrin-dependent cell growth and survival are mediated by different signals in thyroid cells (2003) J. Clin. Endocrinol. Metab., 88 (1), pp. 260-269. , 12519863 10.1210/jc.2002-020774 1:CAS:528:DC%2BD3sXkvVCjsA%3D%3D
Di Matola, T., Mueller, F., Fenzi, G., Rossi, G., Bifulco, M., Marzano, L.A., Vitale, M., Serum withdrawal-induced apoptosis in thyroid cells is caused by loss of fibronectin-integrin interaction (2000) J. Clin. Endocrinol. Metab., 85 (3), pp. 1188-1193. , 10720060 10.1210/jc.85.3.1188
Vitale, M., Casamassima, A., Illario, M., Bassi, V., Fenzi, G., Rossi, G., Cell-to-cell contact modulates the expression of the beta 1 family of integrins in primary cultures of thyroid cells (1995) Exp. Cell Res., 220 (1), pp. 124-129. , 7545127 10.1006/excr.1995.1298 1:CAS:528:DyaK2MXnvVCnsrs%3D
Sasaki, M.S., Advances in the biophysical and molecular bases of radiation cytogenetics (2009) Int. J. Radiat. Biol., 85 (1), pp. 26-47. , 10.1080/09553000802641185 19205983 10.1080/09553000802641185 1:CAS:528:DC%2BD1MXhslSnsrc%3D
Karin, M., Nuclear factor-kappaB in cancer development and progression (2006) Nature, 441 (7092), pp. 431-436. , 10.1038/nature04870 16724054 10.1038/nature04870 1:CAS:528: DC%2BD28XkvVyrsrw%3D
Nucera, C., Porrello, A., Antonello, Z.A., Mekel, M., Nehs, M.A., Giordano, T.J., Gerald, D., Parangi, S., B-Raf(V600E) and thrombospondin-1 promote thyroid cancer progression (2010) Proc. Natl. Acad. Sci. USA, 107 (23), pp. 10649-10654. , 10.1073/pnas.1004934107 20498063 10.1073/pnas.1004934107 1:CAS:528:DC%2BC3cXnvV2it78%3D
Chakravarty, D., Santos, E., Ryder, M., Knauf, J.A., Liao, X.H., West, B.L., Bollag, G., Fagin, J.A., Small-molecule MAPK inhibitors restore radioiodine incorporation in mouse thyroid cancers with conditional BRAF activation (2011) J. Clin. Invest, 121 (12), pp. 4700-4711. , 10.1172/JCI46382 22105174 10.1172/JCI46382 1:CAS:528:DC%2BC3MXhs1ShsLvJ
Vitale, M., Intratumor BRAFV600E heterogeneity and kinase inhibitors in the treatment of thyroid cancer: A call for participation (2013) Thyroid, , In press. doi: 10.1089/thy.2012.0614
Salzano, M., Russo, E., Postiglione, L., Guerra, A., Marotta, V., Esposito, S., Vitale, M., Interferon-gamma inhibits integrin-mediated adhesion to fibronectin and survival signaling in thyroid cells (2012) J. Endocrinol., 215 (3), pp. 439-444. , 10.1530/JOE-12-0335 23027608 10.1530/JOE-12-0335 1:CAS:528: DC%2BC38Xhsl2it7fO
Bonnema, S. J., Hegedus, L., Radioiodine therapy in benign thyroid diseases: Effects, side effects, and factors affecting therapeutic outcome (2012) Endocrinol. Rev., , 10. 1210/er. 2012-103
Sawin, C. T., Becker, D. V., Radioiodine and the treatment of hyperthyroidism: The early history (1997) Thyroid, 7 (2), pp. 163-176. , 9133679 10. 1089/thy. 1997. 7. 163 1: STN: 280: DyaK2s3nvFWksw%3D%3D
Bonnema, S. J., Fast, S., Hegedus, L., Non-surgical approach to the benign nodular goiter: New opportunities by recombinant human TSH-stimulated 131I-therapy (2012) Endocrine, 40 (3), pp. 344-353. , 10. 1007/s12020-011-9542-6 10. 1007/s12020-011-9542-6
Sawka, A. M., Thephamongkhol, K., Brouwers, M., Thabane, L., Browman, G., Gerstein, H. C., Clinical review 170: A systematic review and metaanalysis of the effectiveness of radioactive iodine remnant ablation for well-differentiated thyroid cancer (2004) J. Clin. Endocrinol. Metab., 89 (8), pp. 3668-3676. , 10. 1210/jc. 2003-03116789/8/3668 15292285 10. 1210/jc. 2003-031167 1: CAS: 528: DC%2BD2cXmslKmsbc%3D
Amundson, S. A., Bittner, M., Meltzer, P., Trent, J., Fornace, Jr. A. J., Induction of gene expression as a monitor of exposure to ionizing radiation (2001) Radiat. Res., 156 (5 PART 2), pp. 657-661. , 11604088 10. 1667/0033-7587 (2001) 156 [0657: IOGEAA] 2. 0. CO
2 1: CAS: 528: DC%2BD3MXosV2ks78%3D
Hershman, J. M., Okunyan, A., Rivina, Y., Cannon, S., Hogen, V., Prevention of DNA double-strand breaks induced by radioiodide- (131) I in FRTL-5 thyroid cells (2011) Endocrinology, 152 (3), pp. 1130-1135. , 10. 1210/en. 2010-1163 21190956 10. 1210/en. 2010-1163 1: CAS: 528: DC%2BC3MXktVGrtro%3D
Young, B. A., Baker, T. G., The ultrastructure of rat thyroid glands under experimental conditions in organ culture (1982) J. Anat., 135 (PART 2), pp. 407-412. , 7174511 1: STN: 280: DyaL3s%2Fot1Chtg%3D%3D
Sasaki, M. S., Advances in the biophysical and molecular bases of radiation cytogenetics (2009) Int. J. Radiat. Biol., 85 (1), pp. 26-47. , 10. 1080/09553000802641185 19205983 10. 1080/09553000802641185 1: CAS: 528: DC%2BD1MXhslSnsrc%3D
Lowe, S. W., Cepero, E., Evan, G., Intrinsic tumour suppression (2004) Nature, 432 (7015), pp. 307-315. , 10. 1038/nature03098 15549092 10. 1038/nature03098 1: CAS: 528: DC%2BD2cXpvVCjtLg%3D
Levine, A. J., Momand, J., Finlay, C. A., The p53 tumour suppressor gene (1991) Nature, 351 (6326), pp. 453-456. , 10. 1038/351453a0 2046748 10. 1038/351453a0 1: CAS: 528: DyaK3MXksVaku7g%3D
Radioiodide ((131)I) is routinely used for the treatment of toxic adenoma, Graves' disease, and for ablation of thyroid remnant after thyroidectomy in patients with thyroid cancer. The toxic effects of ionizing radiations on living cells can be mediated by a necrotic and/or apoptotic process. The involvement of apoptosis in radiation-induced cell death in the thyrocytes has been questioned. The knowledge of the mechanisms that underlie the thyrocyte death in response to radiations can help to achieve a successful treatment with the lowest (131)I dose. We developed a method to study the effects of (131)I in human thyroid tissue in culture, by which we demonstrated that (131)I induces thyroid cell apoptosis. Human thyroid tissues of about 1 mm(3) were cultured in vitro and cell viability was determined up to 3 weeks by the MTT assay. Radioiodide added to the culture medium was actively taken up by the tissues. The occurrence of apoptosis in the thyrocytes was assessed by measuring the production of a caspase-cleavage fragment of cytokeratin 18 (M30) by an enzyme-linked immunoassay. Neither variation of cell number nor spontaneous apoptosis was revealed after 1 week of culture. (131)I added to the culture medium induced a dose-dependent and a time-dependent generation of M30 fragment. The apoptotic process was confirmed by the generation of caspase-3 and PARP cleavage products. These results demonstrate that (131)I induces apoptosis in human thyrocytes. Human thyroid tissue cultures may be useful to investigate the cell death pathways induced by (131)I.
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124 Records (108 escludendo Abstract e Conferenze). Impact factor totale: 574.86 (510.173 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 592.287 (519.671 escludendo Abstract e Conferenze).
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