Parole chiave: Magnetic Resonance, Ultrasound Angiography, Mouse, Neck Anatomy,
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*** IBB - CNR ***
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Background<br>Animal models may help in understanding the pathogenesis of many neurological diseases. Recently,<br>vascular abnormalities in Multiple Sclerosis have been suggested as a possible contributing factor, but no<br>studies discussed the anatomy of the mouse head/neck vasculature with a focus on the venous side.<br>Objectives<br>To assess the feasibility of depicting venous extracranial vessels in mice by High-Resolution Ultrasound<br>(HRUS) and non-contrast-enhanced Magnetic Resonance Angiography (MRA). To understand venous<br>anatomy of the mouse compared to human, in order to set-up a model of venous occlusion and test if this<br>may contribute to vascular-related neurological diseases.<br>Methods<br>5 Wild-type mice underwent, under general anaesthesia, transverse and longitudinal HRUS of the neck<br>(Vevo2100 Visualsonics) with a 40 MHz electronic probe, using Color and Power-Doppler modes for blood<br>flow quantification and vessels identification. On a 9.4T Bruker-Biospec scanner, equipped with a volume<br>transmitter coil and four-channel rat brain receiver coil, an axial multi-slice 2D Time-Of-Flight MRA was<br>acquired covering the whole neck and the lower part of the brain.<br>Results<br>Vascular anatomy and haemodynamics, including vascular diameters, lengths, branch points and flow<br>velocities, were measured by HRUS. The integration with MRA permitted a more confident identification of<br>the neck veins and their relationships with the nearby anatomic structures. A complex venous network with<br>bilateral anastomoses connecting the major veins was observed: large external and anterior jugular veins<br>drain the muscle and salivar gland tissue, while small internal jugular vein and vertebral venous plexus drain<br>the cerebral veins.<br>Conclusion<br>HRUS and MRA can provide a complete visualization of the cerebral venous drainage system and its<br>function in the murine neck, quantifying the proportion of blood leaving the brain through the internal<br>jugular and vertebral veins. As the mouse neck venous anatomy is not similar to human, in vivo imaging of<br>normal vessels is necessary before the evaluation of disease models.
Antonini A, Vitale C, Barone P, Cilia R, Righini A, Bonuccelli U, Abbruzzese G, Ramat S, Petrone A, Quatrale R, Marconi R, Ceravolo R, Stefani A, Lopiano L, Zappia M, Capus L, Morgante L, Tamma F, Tinazzi M, Colosimo C, Guerra UP, Valzania F, Fagioli G, Distefano A, Bagnato A, Feggi L, Anna S, Maria Teresa Rosaria De Cr, Nobili F, Mazzuca N, Baldari S, Eleopra R, Bestetti A, Benti R, Varrone A, Volterrani D, Massa R, Stocchi F, Schillaci O, Dore F, Zibetti M, Castellano G, Battista SG, Giorgetti G * The relationship between cerebral vascular disease and parkinsonism: The VADO study(163 visite) Parkinsonism Relat Disord (ISSN: 1353-8020, 1873-5126, 1873-5126electronic), 2012; 18(6): 775-780. Impact Factor:3.274 DettagliEsporta in BibTeXEsporta in EndNote
Malvindi MA, Greco A, Conversano F, Figuerola A, Corti M, Bonora M, Lascialfari A, Doumari HA, Moscardini M, Cingolani R, Gigli G, Casciaro S, Pellegrino T, Ragusa A * MR Contrast Agents(124 visite) Small Animal Imaging, 2011 Jul 8; 21(13): 2548-2555. Impact Factor:1.784 DettagliEsporta in BibTeXEsporta in EndNote
Ntziachristos V, Cuénod CA, Fournier L, Balvay D, Pradel C, Siauve N, Clement O, Jouannot E, Lucidarme O, Vecchio SD, Salvatore M, Law B, Tung C-H, Jain RK, Fukumura D, Munn LL, Brown EB, Schellenberger E, Montet X, Weissleder R, Clerck ND, Postnov A * Tumor Imaging(124 visite) Textbook Of In Vivo Imaging In Vertebrates (ISSN: 9780-4700), 2007 Jul 16; 1: 277-309. Impact Factor:1.148 DettagliEsporta in BibTeXEsporta in EndNote
Hesse B, Tagil K, Cuocolo A, Anagnostopoulos C, Bardies M, Bax J, Bengel F, Busemann Sokole E, Davies G, Dondi M, Edenbrandt L, Franken P, Kjaer A, Knuuti J, Lassmann M, Ljungberg M, Marcassa C, Marie PY, Mckiddie F, O'connor M, Prvuolovich E, Underwood R * 3. 0 T perfusion MR imaging(305 visite) Rivista Di Neuroradiologia (ISSN: 1120-9976), 2004; 17(6): 807-812. Impact Factor:0.023 DettagliEsporta in BibTeXEsporta in EndNote
606 Records (578 escludendo Abstract e Conferenze). Impact factor totale: 2029.706 (1967.469 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 2154.958 (2086.111 escludendo Abstract e Conferenze).
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