Parole chiave: After-Treatment, Electrical Impedance Spectroscopy, Electroporation, Frequency Ranges, Low-Voltage Pulse, Measurement Procedures, Skin Impedances, Square Wave Pulse, Drug Therapy, Electric Potential,
Affiliazioni:
*** IBB - CNR *** Dipartimento di Ingegneria, Università del Sannio, Corso Garibaldi 107, 82100 Benevento, Italy Consiglio Nazionale delle Ricerche, Istituto di Ingegneria Biomedica-Sez. Di Roma, Area di Ricerca Roma 1, Montelibretti, via Salaria km 29,300, 00016 Monterotondo S. (RM), Italy
Riferimenti:
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Regnier, V., Morre, N.D., Jadoul, A., Preat, V., Mechanism of a phosphorothioate oligonucleotide delivery by skin electroporation (1999) Int. J. Pharm, 184, pp. 147-156
Curdy, C., Naik, A., Kalia, Y.N., Alberti, I., Guy, R.H., Non-invasive assessment of the effect of formulation excipients on stratum corneum barrier function in vivo (2004) Int. J. of Pharm, 271, pp. 251-256
Dujardin, N., Staes, E., Kalia, Y., Clarys, P., Guy, R., Preat, V., In vivo assessment of skin electroporation using square wave pulses (2002) J. Control. Release, 79, pp. 219-227
Denet, A.R., Vanbever, R., Preat, V., Skin electroporation for transdermal and topical delivery (2004) Advanced Drug Delivery Reviews, 56, pp. 659-674
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Arpaia, Clemente, F., Romanucci, C., Impedance spectroscopy-based measurement of cochlear prosthesis osseointegration: In-vivo test procedure and characterization (2007) Proc. of IMTC, pp. 1786-1791. , Warsaw (Poland)
Prausnitz, M. R., Bose, V. G., Langer, R., Weaver, J. C., Electroporation of mammalian skin: A mechanism to enhance transdermal drug delivery (1993) Proc. Natl. Acad. Sci. USA, 90, pp. 10504-1050
Riviere, J. E., Monteiro-Riviere, N. A., Rogers, R. A., Bommannan, D., Tamada, J. A., Potts, R. O., Pulsatile transdermal delivery of LHRH using electroporation: Drug delivery and skin toxicology (1995) J. Control. Release, 36, pp. 229-233
Titomirov, A. V., Sukharev, S., Kistanova, E., In vivo electroporation and stable transformation of skin cells of new born mice by plasmid DNA (1991) Biochem. Biophys. Acta, 1088, pp. 131-134
Mir, L. M., Bureau, M. F., Gehl, J., Rangara, R., Rouy, D., Caillaud, J. M., Delaere, P., Sherman, D., High efficiency gene transfer into skeletal muscle mediated by electric pulses (1999) Proc. Natl. Acad. Sci. USA, 96 (8), pp. 4262-4267
Denet, A. R., Vanbever, R., Preat, V., Skin electroporation for transdermal and topical delivery (2004) Advanced Drug Delivery Reviews, 56, pp. 659-674
The aim of this work is to measure the skin impedance recovery after electroporation by using low-voltage pulse protocol on human subject. Before and after electroporation, based on square wave pulses of 20-50 V, 5 μs, for 15-20 minutes, Electrical Impedance Spectroscopy (EIS) measurements has been performed, in the frequency range 0.5-10 kHz. Preliminary experimental results show a significant decrease in skin impedance amplitude after treatment. This result turns out to be useful in a measurement procedure aimed at determining the amount of drug actually transferred in electroporation-based therapy.
12 Records (10 escludendo Abstract e Conferenze). Impact factor totale: 26.616 (20.254 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 26.176 (19.816 escludendo Abstract e Conferenze).
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