Home Ricerca Sedi Chi siamo Organigramma Personale Contatti Didattica Login Documenti EN
Progetti in evidenza
INCIPIT
(Link esterno)

ISTAPCA
(1174 visite)

Fondo Europeo Pesca
(587 visite)

Euro-BioImaging
(Link esterno)

Fondazione Veronesi
(Link esterno)

LightDyNAmics
(1045 visite)

 INMiND
(1157 visite)

Instruct-IT
(Link esterno)

 PRIN
(1291 visite)

 eHealthNet
(1059 visite)

Ponrec
(1002 visite)

MFAG Grant
(1095 visite)

MERIT
(Link esterno)


Collegamenti



Structural and functional studies of insulin receptors in human breast cancer (97 visite)

FRITTITTA L, VIGNERI R, PAPA V, GOLDFINE I, GRASSO G, TRISCHITTA V

Breast Cancer Res Tr Kluwer Academic Publishers (ISSN: 0167-6806), 1993; 25(1): 73-82.

Tipo di articolo: Journal Article,

Impact factor: 2.43

Impact factor a 5 anni: 4.024


Parole chiave: Growth Factors, Insulin Sensitivity, Oncogenes, Receptor Growth Factors, Tyrosine Kinase, Adenosine Triphosphate, Insulin Receptor, Messenger Rna, Protein Tyrosine Kinase, Alpha Chain, Article, Autophosphorylation, Beta Chain, Breast, Breast Cancer, Comparative Study, Controlled Study, Cross Linking, Enzyme Activity, Female, Human, Human Tissue, Immunoprecipitation, Polyacrylamide Gel Electrophoresis, Radioimmunoassay, Receptor Binding, Structure Activity Relation, Breast Neoplasms, Peptide Fragments, Protein-Tyrosine Kinase, Reference Values, Structure-Activity Relationship, Support, Non-U.S. Gov, Non-U. S. Gov,

Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-0027409865&partnerID=40&md5=f259967832a3cc6279dcefb46f1d2853

We characterized the structure and the function of insulin receptors isolated from 10 human breast cancer specimens. We observed that the insulin receptor content, as determined by a specific radioimmunoassay, was four fold increased in human breast cancer tissue when compared to normal breast tissues. In both cancer and normal breast tissues, insulin receptor mRNA consisted of two major species of approximately 11.0 and 8.5 kilobases. The size of the insulin receptor alpha subunit was determined by125I-insulin cross-linking followed by immunoprecipitation and polyacrylamide gel electrophoresis; a value of 135 kDa was observed for receptors from both breast cancer and normal breast tissues. The functional binding ability of insulin receptors from cancer tissues was slightly lower as compared to normal tissue derived insulin receptor (% B/T= 2.22±0.50 per ng of insulin receptor as determined by radioimmunoassay vs. 2.96±0.49, mean±S.E.M.). The concentration of insulin that caused half maximal inhibition of125I-insulin binding was very similar for both cancer and normal breast receptors (80pM). The size of the insulin receptor beta subunit as determined by receptor autophosphorylation was 95kDa. Basal and maximal insulin (100nM) stimulated receptor tyrosine kinase activity, in terms of both receptor autophosphorylation and phosphorylation of an exogenous substrate, was similar in malignant and normal breast tissue derived insulin receptor. Also, a very similar insulin stimulated Km value for ATP was showed by the tyrosine kinase of insulin receptors from breast cancer and normal breast tissue (11.1 and 10.8μM ATP, respectively). However, in insulin receptors from breast cancer tissue the average tyrosine kinase sensitivity to insulin, as calculated on the exogenous substrate, was higher, although not significantly, with respect to normal breast tissue (ED50 at 0.28±0.09 and 1.08±0.33 nM insulin, respectively). A similarly different sensitivity to insulin was observed also for receptor autophosphorylation. In conclusion, this study demonstrates that breast cancer tissues have an increased number of structurally and functionally normal insulin receptors. In some breast cancer tissues, however, the sensitivity of the receptor tyrosine kinase activity to insulin is greatly increased. These data suggest that, in vivo, the mitogenic effect of insulin may play a role in the biology of certain breast cancers. © 1993 Kluwer Academic Publishers.
Cattedra di Endocrinologia e Patologia Costituzionale, Università di Catania, Ospedale Garibaldi, USL 34, Piazza S.M. di Gesù, Catania, 95123, Italy

Diabetes and Endocrine Research, Mount Zion Medical Center, and Department of Medicine and Physiology, University of California San Francisco, San Francisco, 94120, CA, United States
Non disponibile.
Nessun risultato.
Nessun risultato.

Contiene: [X]      Estesa         Autori: [X]      Tipo di lavoro: [X]
Data iniziale: Data finale: [X]      Sede: Affiliazione IBB     
    
[Pulisci modulo]
De Rosa V, Iommelli F, Monti M, Mainolfi CG, Fonti R, Del Vecchio S
* Early 18F-FDG uptake as a reliable imaging biomarker of T790M-mediated resistance but not MET amplification in non-small cell lung cancer treated with EGFR tyrosine kinase inhibitors (93 visite)
Ejnmmi Res, 2016 Dec; 6(1): 74-74.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Miceli M, Dell'Aversana C, Russo R, Rega C, Cupelli L, Ruvo M, Altucci L, Chambery A
* Secretome profiling of cytokines and growth factors reveals that neuro-glial differentiation is associated with the down-regulation of Chemokine Ligand 2 (MCP-1/CCL2) in amniotic fluid derived-mesenchymal progenitor cells (91 visite)
Proteomics (ISSN: 1615-9853), 2016; 16(4): 674-688.
Dettagli    Esporta in BibTeX    Esporta in EndNote

De Rosa V, Iommelli F, Monti M, Fonti R, Del Vecchio S
* Early reduction of 18F-FDG uptake in non-small lung cancer treated with EGFR tyrosine kinase inhibitors reflects reversal of Warburg effect and reactivation of oxidative phosphorylation (94 visite)
Eur J Nucl Med (ISSN: 1619-7070, 1619-7089, 0340-6997), 2014 Oct; 41: N/D-N/D.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Iommelli F, De Rosa V, Gargiulo S, Panico M, Monti M, Greco A, Gramanzini M, Ortosecco G, Fonti R, Brunetti A, Del Vecchio S
* Monitoring Reversal of MET-Mediated Resistance to EGFR Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancer Using 3 '-Deoxy-3 '-[F-18]-Fluorothymidine Positron Emission Tomography (179 visite)
Clin Cancer Res (ISSN: 1078-0432, 1078-0432linking, 1078-0432print), 2014 Sep 15; 20(18): 4806-4815.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Iommelli F, De Rosa V, Fonti R, Del Vecchio S
* Molecular Imaging For Detection Of Sensitivity And Resistance To Egfr Tyrosine Kinase Inhibitors In Non-Small Cell Lung Cancer (113 visite)
Clin Transl Imaging (ISSN: 2281-5872print, 2281-7565online), 2014 Feb 20; 2(1): 43-53.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Zannetti A, Iommelli F, Fonti R, Lettieri A, Pirozzi G, Bianco R, Tortora G, Salvatore M, Del Vecchio S
* Imaging of Bcl-2/Bcl-x(L) dysregulated apoptotic program in NSCLC cells resistant to EGFR tyrosine kinase inhibitors (90 visite)
Eur J Nucl Med (ISSN: 1619-7070, 1619-7089, 0340-6997), 2009 Sep; 36: N/D-N/D.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Zannetti A, Iommelli F, Fonti R, Papaccioli A, Sommella J, Lettieri A, Bianco R, Tortora G, Salvatore M, Del Vecchio S
* Imaging of lung cancer resistance to EGFR tyrosine kinase inhibitors through Bcl-2/Bcl-x(L) modulation of inositol trisphosphate receptor type (95 visite)
Eur J Nucl Med (ISSN: 1619-7070, 1619-7089, 0340-6997), 2008 Oct; 35: N/D-N/D.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Viparelli F, Cassese A, Doti N, Paturzo F, Marasco D, Dathan NA, Monti SM, Basile G, Ungaro P, Sabatella M, Miele C, Teperino R, Consiglio E, Pedone C, Beguinot F, Formisano P, Ruvo M
* Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (99 visite)
J Biol Chem Journal Of Biological Chemistry (ISSN: 0021-9258, 1083-351x), 2008 Sep 1; 283(31): 21769-21778.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Migliore T, Parrella LS, Caputi A, Silvestri N, Romano R, Pace L, Imbriaco M, Losi MA, Betocchi S
* Pathogenesis of hypertrophic cardiomyopathy. Impact of growth factors on left ventricular anatomy (103 visite)
Minerva Cardioangiol (ISSN: 0026-4725), 2008 Feb; 56(1): 13-20.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Zannetti A, Iommelli F, Fonti R, Papaccioli A, Sommella J, Lettieri A, Bianco R, Tortora G, Salvatore M, Del Vecchio S
* Imaging Of Lung Cancer Resistance To Egfr Tyrosine Kinase Inhibitors Trough Bcl-2/Bcl-Xl Modulation Of Inositol Trisphosphate Receptor Type (95 visite)
Eur J Nucl Med, 2008; suppl2: N/D-N/D.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Del Vecchio S, Zannetti A, Iommelli F, Papaccioli A, Sommella J, Ferraro P, Baiano A, Salvatore M
* In vivo detection of early tumor response to tyrosine kinase inhibitors by microSPECT with 99mTc-MIBI (94 visite)
Eur J Nucl Med (ISSN: 1619-7070, 1619-7089, 0340-6997), 2006 Sep; 33: N/D-N/D.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Del Vecchio S, Zannetti A, Fonti R, Iommelli F, Sommella I, Potena A, Salvatore M
* 99mTc-MIBI uptake in Bcl-2 overexpressing breast cancer cells during treatment with gefitinib, a selective tyrosine kinase inhibitor (87 visite)
Eur J Nucl Med (ISSN: 1619-7070, 1619-7089, 0340-6997), 2005 Sep; 32: N/D-N/D.
Dettagli    Esporta in BibTeX    Esporta in EndNote

Zannetti A, Del Vecchio S, Fonti R, Iommelli F, Sommella J, Potena A, Salvatore M
* Gefitinib, A Selective, Tyrosine Kinase Inhibitor Increases 99mtc-Mibi Uptake In Bcl-2 Overexpressing Breast Cancer Cells (80 visite)
Mol Immunol, 2005; 4: 355-355.
Dettagli    Esporta in BibTeX    Esporta in EndNote



13 Records (6 escludendo Abstract e Conferenze).
Impact factor totale: 51.461 (20.525 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 50.917 (18.466 escludendo Abstract e Conferenze).







    Esporta in BibTeX    Esporta in EndNote

Ultima modifica di Marco Comerci in data Thursday 19 March 2015, 14:45:36
97 visite. Ultima visita in data Saturday 27 October 2018, 10:00:08

Webmaster and developer: Marco Comerci
Per problemi e suggerimenti: adminibb.cnr.it
Ultimo aggiornamento: Monday 12 November 2018, 16:59:37