Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15 (421 views)
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.621, 5-year impact factor: 1.761
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-42749089546&partnerID=40&md5=17dd299046dc8eb5c67b73e880bcba43
Keywords: Phospholipase, Phosphoprotein Enriched In Diabetes (ped), Protein-Protein Interaction, Pea15 Protein, Human, Phospholipase D1, Signal Peptide, Article, Biosynthesis, Chemistry, Enzyme Linked Immunosorbent Assay, Genetics, Isolation And Purification, Molecular Cloning, Protein Tertiary Structure, Surface Plasmon Resonance, Enzyme-Linked Immunosorbent Assay, Intracellular Signaling Peptides And Proteins, Protein Structure,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini (IBB), CNR, via Mezzocannone, 16, 80134 Napoli, Italy
Dipartimento di Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone, 16, 80134 Napoli, Italy
Dipartimento di Biochimica e Biofisica, Seconda Università di Napoli, Via S.M. di Costantinopoli 16, 80138 Napoli, Italy
Istituto di Endocrinologia ed Oncologia Sperimentale (IEOS), CNR, Via Pansini, 5, 80131 Napoli, Italy
References:
McDermott, M., Wakelam, M.J.O., Morris, A.J., Phospholipase D 1,2, Biochem (2004) Cell Biol., 82, pp. 225-25
Besterman, J.M., Duronio, V., Cuatrecasa, P., Rapid formation of diacylglycerol from phosphatidylcholine: a pathway for generation of a second messenger (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 6785-6789
Sung, T.C., Zhang, Y., Morris, A.J., Frohman, M.A., Structural analysis of human phospholipase D1 (1999) J. Biol. Chem., 274, pp. 3659-3666
Sung, T.C., Altshuller, Y.M., Morris, A.J., Frohman, M.A., Molecular analysis of mammalian phospholipase D2 (1999) J. Biol. Chem., 274, pp. 494-502
Sung, T.C., Roper, R., Zhang, Y., Rudge, S.A., Temel, R., Hammond, S.M., Morris, A.J., Frohman, M.A., Mutagenesis of Phospholipase D defines a superfamily including a trans-golgi viral protein required for poxvirus pathogenicity (1997) EMBO J., 16, pp. 4519-4539
Stuckey, J.A., Dixon, J.E., Crystal structure of a phospholipase D member (1999) Nat. Struct. Biol., 6, pp. 278-284
Davies, D.R., Interthal, H., Champoux, J.J., Hol, W.G., The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1 (2002) Structure, 10, pp. 237-248
Leiros, I., Secundo, F., Zambonelli, C., Servi, S., Hough, E., The first crystal structure of a phospholipase D (2000) Structure, 8, pp. 655-667
Ponting, C.P., Kerr, I.D., A novel family of phospholipase D homologues that includes phospholipid synthases and putative endonucleases: identification of duplicated repeats and potential active site residues (1996) Protein Sci., 5, pp. 914-922
Xie, Z., Ho, W.T., Exton, J., Association of the N- and C-terminal domains of Phospholipase D (2000) J. Biol. Chem., 275, pp. 24962-24969
Kam, Y., Exton, J.H., Dimerization of phospholipase D isozymes (2002) Biochem. Biophys. Res. Commun., 290, pp. 375-380
Exton, J.H., Phospholipase D-structure, regulation and function (2002) Rev. Physiol. Biochem. Pharmacol., 144, pp. 1-94
Zhang, Y., Redina, O., Altshuller, Y.M., Yamazaki, M., Ramos, J., Chneiweiss, H., Kanaho, Y., Frohman, M.A., Regulation of expression of Phospholipase D1 and D2 by PEA-15, a novel protein that interacts with them (2000) J. Biol. Chem., 275, pp. 35224-35232
Vigliotta, G., Miele, C., Santopietro, S., Portella, G., Perfetti, A., Maitan, M.A., Cassese, A., Beguinot, F., Overexpression of the PED/PEA15 gene causes diabetes by impairing glucose-stimulated insulin secretion in addition to insulin action (2004) Mol. Cell. Biol., 24, pp. 5005-5015
Condorelli, G., Vigliotta, G., Iavarone, C., Caruso, M., Tocchetti, C.G., Andreozzi, F., Cafieri, A., Beguinot, F., PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus (1998) EMBO J., 17, pp. 3858-3866
Xiao, C., Yang, B.F., Asadi, N., Beguinot, F., Hao, C., Tumor necrosis factor-related apoptosis-inducing signaling complex and its modulation by c-Flip and PED/PEA-15 in glioma cells (2002) J. Biol. Chem., 277 (28), pp. 25020-25025
Condorelli, G., Trencia, A., Vigliotta, G., Perfetti, A., Goglia, U., Cassese, A., Musti, A.M., Beguinot, F., Multiple members of the mitogen-activated protein kinase family are necessary for PED/PEA15 anti-apoptotic function (2002) J. Biol. Chem., 277, pp. 11013-11018
Johnsson, B., Lofas, S., Lindquist, G., Immobilization of proteins to a carboxylmethyl dextran modified gold surface for biospecific interaction analysis in SPR sensors (1991) Anal. Biochem., 198, pp. 268-277
Hill, J.M., Vaidyanathan, H., Ramos, J.W., Ginsberg, M.H., Werner, M.H., Recognition of ERK MAP kinase by PEA-15 reveals a common docking site within the death domain and death effector domain (2002) EMBO J., 21, pp. 6494-6504
Protein Expr Purif (ISSN: 1046-5928), 2008 Jun; 59(2): 302-308.
Export to BibTeX Export to EndNote
Paper type: Journal Article,
Impact factor: 1.621, 5-year impact factor: 1.761
Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-42749089546&partnerID=40&md5=17dd299046dc8eb5c67b73e880bcba43
Keywords: Phospholipase, Phosphoprotein Enriched In Diabetes (ped), Protein-Protein Interaction, Pea15 Protein, Human, Phospholipase D1, Signal Peptide, Article, Biosynthesis, Chemistry, Enzyme Linked Immunosorbent Assay, Genetics, Isolation And Purification, Molecular Cloning, Protein Tertiary Structure, Surface Plasmon Resonance, Enzyme-Linked Immunosorbent Assay, Intracellular Signaling Peptides And Proteins, Protein Structure,
Affiliations:
*** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini (IBB), CNR, via Mezzocannone, 16, 80134 Napoli, Italy
Dipartimento di Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone, 16, 80134 Napoli, Italy
Dipartimento di Biochimica e Biofisica, Seconda Università di Napoli, Via S.M. di Costantinopoli 16, 80138 Napoli, Italy
Istituto di Endocrinologia ed Oncologia Sperimentale (IEOS), CNR, Via Pansini, 5, 80131 Napoli, Italy
References:
McDermott, M., Wakelam, M.J.O., Morris, A.J., Phospholipase D 1,2, Biochem (2004) Cell Biol., 82, pp. 225-25
Besterman, J.M., Duronio, V., Cuatrecasa, P., Rapid formation of diacylglycerol from phosphatidylcholine: a pathway for generation of a second messenger (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 6785-6789
Sung, T.C., Zhang, Y., Morris, A.J., Frohman, M.A., Structural analysis of human phospholipase D1 (1999) J. Biol. Chem., 274, pp. 3659-3666
Sung, T.C., Altshuller, Y.M., Morris, A.J., Frohman, M.A., Molecular analysis of mammalian phospholipase D2 (1999) J. Biol. Chem., 274, pp. 494-502
Sung, T.C., Roper, R., Zhang, Y., Rudge, S.A., Temel, R., Hammond, S.M., Morris, A.J., Frohman, M.A., Mutagenesis of Phospholipase D defines a superfamily including a trans-golgi viral protein required for poxvirus pathogenicity (1997) EMBO J., 16, pp. 4519-4539
Stuckey, J.A., Dixon, J.E., Crystal structure of a phospholipase D member (1999) Nat. Struct. Biol., 6, pp. 278-284
Davies, D.R., Interthal, H., Champoux, J.J., Hol, W.G., The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1 (2002) Structure, 10, pp. 237-248
Leiros, I., Secundo, F., Zambonelli, C., Servi, S., Hough, E., The first crystal structure of a phospholipase D (2000) Structure, 8, pp. 655-667
Ponting, C.P., Kerr, I.D., A novel family of phospholipase D homologues that includes phospholipid synthases and putative endonucleases: identification of duplicated repeats and potential active site residues (1996) Protein Sci., 5, pp. 914-922
Xie, Z., Ho, W.T., Exton, J., Association of the N- and C-terminal domains of Phospholipase D (2000) J. Biol. Chem., 275, pp. 24962-24969
Kam, Y., Exton, J.H., Dimerization of phospholipase D isozymes (2002) Biochem. Biophys. Res. Commun., 290, pp. 375-380
Exton, J.H., Phospholipase D-structure, regulation and function (2002) Rev. Physiol. Biochem. Pharmacol., 144, pp. 1-94
Zhang, Y., Redina, O., Altshuller, Y.M., Yamazaki, M., Ramos, J., Chneiweiss, H., Kanaho, Y., Frohman, M.A., Regulation of expression of Phospholipase D1 and D2 by PEA-15, a novel protein that interacts with them (2000) J. Biol. Chem., 275, pp. 35224-35232
Vigliotta, G., Miele, C., Santopietro, S., Portella, G., Perfetti, A., Maitan, M.A., Cassese, A., Beguinot, F., Overexpression of the PED/PEA15 gene causes diabetes by impairing glucose-stimulated insulin secretion in addition to insulin action (2004) Mol. Cell. Biol., 24, pp. 5005-5015
Condorelli, G., Vigliotta, G., Iavarone, C., Caruso, M., Tocchetti, C.G., Andreozzi, F., Cafieri, A., Beguinot, F., PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus (1998) EMBO J., 17, pp. 3858-3866
Xiao, C., Yang, B.F., Asadi, N., Beguinot, F., Hao, C., Tumor necrosis factor-related apoptosis-inducing signaling complex and its modulation by c-Flip and PED/PEA-15 in glioma cells (2002) J. Biol. Chem., 277 (28), pp. 25020-25025
Condorelli, G., Trencia, A., Vigliotta, G., Perfetti, A., Goglia, U., Cassese, A., Musti, A.M., Beguinot, F., Multiple members of the mitogen-activated protein kinase family are necessary for PED/PEA15 anti-apoptotic function (2002) J. Biol. Chem., 277, pp. 11013-11018
Johnsson, B., Lofas, S., Lindquist, G., Immobilization of proteins to a carboxylmethyl dextran modified gold surface for biospecific interaction analysis in SPR sensors (1991) Anal. Biochem., 198, pp. 268-277
Hill, J.M., Vaidyanathan, H., Ramos, J.W., Ginsberg, M.H., Werner, M.H., Recognition of ERK MAP kinase by PEA-15 reveals a common docking site within the death domain and death effector domain (2002) EMBO J., 21, pp. 6494-6504
Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15
PLD's (Phospholipases D) are ubiquitously expressed proteins involved in many transphosphatidylation reactions. They have a bi-lobed structure composed by two similar domains which at their interface reconstitute the catalytic site through the association of the two conserved HxKx4Dx6GSxN motifs. PLD1 interacts with the small phosphoprotein PED-PEA15 by an unknown mechanism that, by enhancing PLD1 stability, apparently increases its enzymatic activity; the minimum interacting region of PLD1 was previously identified as spanning residues 712-1074 (D4 region). Since the D4/PED-PEA15 interaction has been claimed to be one of the multiple molecular events that can trigger type 2 diabetes, we purified the two recombinant proteins to study in vitro this binding by both ELISA and SPR techniques. Whilst PED-PEA15 was easily expressed and purified, expression of recombinant D4 was more problematic and only the fusion protein with Thioredoxin A and a six Histidine Tag (Trx-His6-D4) demonstrated sufficient stability for further characterization. We have found that Trx-His6-D4 is present as two different oligomeric forms, though only the monomeric variant is able to interact with PED-PEA15. All these findings may have important implications for both the mechanisms of phospholipase activity and PED-PEA15 regulative functions. © 2008 Elsevier Inc. All rights reserved.
PLD's (Phospholipases D) are ubiquitously expressed proteins involved in many transphosphatidylation reactions. They have a bi-lobed structure composed by two similar domains which at their interface reconstitute the catalytic site through the association of the two conserved HxKx4Dx6GSxN motifs. PLD1 interacts with the small phosphoprotein PED-PEA15 by an unknown mechanism that, by enhancing PLD1 stability, apparently increases its enzymatic activity; the minimum interacting region of PLD1 was previously identified as spanning residues 712-1074 (D4 region). Since the D4/PED-PEA15 interaction has been claimed to be one of the multiple molecular events that can trigger type 2 diabetes, we purified the two recombinant proteins to study in vitro this binding by both ELISA and SPR techniques. Whilst PED-PEA15 was easily expressed and purified, expression of recombinant D4 was more problematic and only the fusion protein with Thioredoxin A and a six Histidine Tag (Trx-His6-D4) demonstrated sufficient stability for further characterization. We have found that Trx-His6-D4 is present as two different oligomeric forms, though only the monomeric variant is able to interact with PED-PEA15. All these findings may have important implications for both the mechanisms of phospholipase activity and PED-PEA15 regulative functions. © 2008 Elsevier Inc. All rights reserved.
Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15
Expression and purification of the D4 region of PLD1 and characterization of its interaction with PED-PEA15
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Tornatore L, Marasco D, Dathan N, Vitale RM, Benedetti E, Papa S, Franzoso G, Ruvo M, Monti SM
* Gadd45 beta forms a homodimeric complex that binds tightly to MKK7 (431 views)
J Mol Biol (ISSN: 0022-2836, 0022-2283, 1089-8638), 2008 Apr 18; 378(1): 97-111.
Impact Factor: 5.673
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* A Molecular Dynamics Study of Pilus Subunits: Insights into Pilus Biogenesis (396 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2007 Apr 6; 367(4): 935-941.
Impact Factor: 4.472
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Galdiero S, Galdiero M, Pedone C
* Beta-Barrel Membrane Bacterial Proteins: Structure, Function, Assembly And Interaction With Lipids (399 views)
Curr Protein Pept Sci (ISSN: 1389-2037, 1389-2037linking), 2007 Feb; 8(1): 63-82.
Impact Factor: 3.259
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Chiechio S, Copani A, Gereau R, Nicoletti F
* Acetyl-l-carnitine in neuropathic pain (263 views)
Cns Drugs (ISSN: 1172-7047), 2007; 21: 38-38.
Impact Factor: 4.514
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* Protein Interaction Domains: structural features and drug discovery applications (part 2) (68 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2021; 28(5): 854-892.
Impact Factor: 4.184
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Tornatore L, Capece D, Sandomenico A, Verzella D, Vecchiotti D, Zazzeroni F, Ruvo M, Franzoso G
* The Screening of Combinatorial Peptide Libraries for Targeting Key Molecules or Protein-Protein Interactions in the NF-κB Pathway (6 views)
Methods Mol Biol (ISSN: 1064-3745linking, 1940-6029electronic), 2021; 2366: 343-356.
Impact Factor: 1.17
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Mercurio FA, Di Natale C, Pirone L, Vincenzi M, Marasco D, De Luca S, Pedone EM, Leone M
* Exploring the ability of cyclic peptides to target SAM domains: a computational and experimental study (244 views)
Chembiochem (ISSN: 1439-4227linking, 1439-7633), 2020 Mar 2; 21(5): 702-711.
Impact Factor: 2.641
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Vincenzi M, Mercurio FA, Leone M
* Sam Domains in Multiple Diseases (224 views)
Curr Med Chem (ISSN: 0929-8673linking, 1875-533xelectronic), 2020; 27(3): 450-476.
Impact Factor: 3.469
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Rega C, Russo R, Foca A, Sandomenico A, Iaccarino E, Raimondo D, Milanetti E, Tornatore L, Franzoso G, Pedone PV, Ruvo M, Chambery A
* Probing the interaction interface of the GADD45beta/MKK7 and MKK7/DTP3 complexes by chemical cross-linking mass spectrometry (258 views)
Int J Biol Macromol (ISSN: 1879-0003electronic, 0141-8130linking), 2018 Jul 15; 114: 114-123.
Impact Factor: 1.427
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Pirone L, Ripoll-rozada J, Leone M, Ronca R, Lombardo F, Fiorentino G, Andersen JF, Pereira PJB, Arcà B, Pedone E
* Functional analyses yield detailedinsight into the mechanism of thrombin inhibition by the antihemostatic salivary protein cE5 from Anopheles gambiae (344 views) (PDF 188 views)
Jbc Papers (ISSN: 0021-9258linking, 1083-351x), 2017 Jul 28; 292(30): 12632-12642.
Impact Factor: 4.125
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Sacchi S, Cappelletti P, Pirone L, Smaldone G, Pedone E, Pollegioni L
* Elucidating the role of the pLG72 R30K substitution in schizophrenia susceptibility (254 views)
Febs Lett (ISSN: 1873-3468, 0014-5793, 0014-5793print), 2017 Feb; 591(4): 646-655.
Impact Factor: 2.999
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Lanza V, Travaglia A, Malgieri G, Fattorusso R, GrassoG, Di Natale G, Zito V, Arena G, Milardi D, Rizzarelli E
* Ubiquitin Associates with the N-Terminal Domain of Nerve Growth Factor: The Role of Copper(II) Ions (264 views) (PDF 168 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2016 Dec 05; 22(49): 17767-17775.
Impact Factor: 5.317
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Pirone L, Smaldone G, Esposito C, Balasco N, Petoukhov MV, Spilotros A, Svergun DI, Di Gaetano S, Vitagliano L, Pedone E
* Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners (304 views) (PDF 187 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2016 Sep 24; 131: 106-114.
Impact Factor: 3.112
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Birolo L, Sacchi S, Smaldone G, Molla G, Leo G, Caldinelli L, Pirone L, Eliometri P, Di Gaetano S, Orefice I, Pedone E, Pucci P, Pollegioni L
* Regulating levels of the neuromodulator d-serine in human brain: structural insight into pLG72 and d-amino acid oxidase interaction (244 views) (PDF 203 views)
Febs J (ISSN: 1742-464x), 2016 Sep; 283(18): 3353-3370.
Impact Factor: 3.902
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Ruvo M, Doti N, Lorenzo V, Vitagliano L, Mascanzoni F
* The Interacting Domains of PREP1 and p160 are Endowed with a Remarkable Structural Stability (259 views)
Mol Biotechnol (ISSN: 1073-6085, 1559-0305electronic, 1073-6085linking), 2016 May; 58(5): 328-339.
Impact Factor: 1.634
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Bottini A, Wu B, Barile E, De SK, Leone M, Pellecchia M
* High-Throughput Screening (HTS) by NMR Guided Identification of Novel Agents Targeting the Protein Docking Domain of YopH (398 views)
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7179linking), 2015 Nov 23; 11(8): 919-927.
Impact Factor: 2.98
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Correale S, de Paola I, Morgillo CM, Federico A, Zaccaro L, Pallante P, Galeone A, Fusco A, Pedone E, Luque FJ, Catalanotti B
* Structural Model of the hUbA1-UbcH10 Quaternary Complex: In Silico and Experimental Analysis of the Protein-Protein Interactions between E1, E2 and Ubiquitin (402 views)
Plosone (ISSN: 1932-6203, 1932-6203electronic, 1932-6203linking), 2014 Nov 6; 9(11): e112082-e112082.
Impact Factor: 3.234
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Balasco N, Pirone L, Smaldone G, Di Gaetano S, Esposito L, Pedone E, Vitagliano L
* Molecular recognition of Cullin3 by KCTDs: Insights from experimental and computational investigations (336 views)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014 Jul; 1844(7): 1289-1298.
Impact Factor: 4.882
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Diana D, Smaldone G, De Antonellis P, Pirone L, Carotenuto M, Alonzi A, Di Gaetano S, Zollo M, Pedone E, Fattorusso R
* Mapping Functional Interaction Sites of Human Prune C-Terminal Domain by NMR Spectroscopy in Human Cell Lysates (339 views)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2013 Sep 9; 19(37): 12217-12220.
Impact Factor: 5.696
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Farina B, Doti N, Pirone L, Malgieri G, Pedone E, Ruvo M, Fattorusso R
* Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy (444 views)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(8): 1572-1580.
Impact Factor: 3.191
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Attanasio F, Convertino M, Magno A, Caflisch A, Corazza A, Haridas H, Esposito G, Cataldo S, Pignataro B, Milardi D, Rizzarelli E
* Carnosine Inhibits Abeta(42) Aggregation By Perturbing The H-Bond Network In And Around The Central Hydrophobic Cluster (583 views)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-7633electronic), 2013 Mar 18; 14(5): 583-592.
Impact Factor: 3.06
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Grasso G, Spoto G
* Plasmonics for the study of metal ion-protein interactions (370 views)
Anal Bioanal Chem (ISSN: 1618-2642), 2013 Feb; 405(6): 1833-1843.
Impact Factor: 3.578
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Mercurio FA, Marasco D, Pirone L, Scognamiglio PL, Pedone E, Pellecchia M, Leone M
* Heterotypic Sam-Sam Association Between Odin-Sam1 And Arap3-Sam: Binding Affinity And Structural Insights (328 views)
Chembiochem (ISSN: 1439-4227, 1439-9422, 1439-7633), 2013 Jan 2; 14(1): 100-106.
Impact Factor: 2.92
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Poletto M, Vascotto C, Scognamiglio PL, Lirussi L, Marasco D, Tell G
* Role Of The Unstructured N-Terminal Domain Of The Human Apurinic/Apyrimidinic Endonuclease 1 (hape1) In The Modulation Of Its Interaction With Nucleic Acids And Nucleophosmin (npm1) (417 views)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2013; 452(3): 545-557.
Impact Factor: 4.779
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Viparelli F, Doti N, Sandomenico A, Marasco D, Dathan NA, Miele C, Beguinot F, Monti SM, Ruvo M
* GSM48878: CD8 IL2-D2 (377 views)
Gene Expression Omnibus, 2011 Dec 2; 59(2): 302-308.
Impact Factor: 2.082
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Ruvo M, Sandomenico A, Tudisco L, De Falco S
* Branched peptides for the modulation of protein-protein interactions: More arms are better than one? (492 views)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2011 Jun; 18(16): 2429-2437.
Impact Factor: 4.859
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Scognamiglio PL, Doti N, Grieco P, Pedone C, Ruvo M, Marasco D
* Discovery Of Small Peptide Antagonists Of Ped/Pea15-D4alpha Interaction From Simplified Combinatorial Libraries (444 views)
Chem Biol Drug Des (ISSN: 1747-0277, 1747-0285), 2011 May; 77(5): 319-327.
Impact Factor: 2.282
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Correale S, Pirone L, Di Marcotullio L, De Smaele E, Greco A, Mazzà D, Moretti M, Alterio V, Vitagliano L, Di Gaetano S, Gulino A, Pedone E
* Molecular organization of the cullin E3 ligase adaptor KCTD11 (404 views)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2011 Apr; 93(4): 715-724.
Impact Factor: 3.022
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Farina B, Pirone L, Russo L, Viparelli F, Doti N, Pedone C, Pedone E, Fattorusso R
* NMR backbone dynamics studies of human PED/PEA-15 outline protein functional sites (639 views)
Febs J (ISSN: 1742-4658, 1742-464x, 1742-4658electronic), 2010 Oct; 277(20): 4229-4240.
Impact Factor: 3.129
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Doti N, Cassese A, Marasco D, Paturzo F, Sabatella M, Viparelli F, Dathan N, Monti SM, Miele C, Formisano P, Beguinot F, Ruvo M
* Residues 762-801 of PLD1 mediate the interaction with PED/PEA15 (457 views)
Mol Biosyst (ISSN: 1742-206x), 2010 Oct; 6(10): 2039-2048.
Impact Factor: 3.825
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Sandomenico A, Monti SM, Sabatella M, De Capua A, Tornatore L, Doti N, Viparelli F, Dathan NA, Pedone C, Ruvo M, Marasco D
* Protein-protein interactions: a simple strategy to identify binding sites and peptide antagonists (439 views)
Chem Biol Drug Des (ISSN: 1747-0285, 1747-0277), 2009 May; 73(5): 483-493.
Impact Factor: 2.473
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Molecular dynamics studies of the P pilus rod subunit PapA (427 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 192-199.
Impact Factor: 1.807
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Viparelli F, Doti N, Monti SM, Marasco D, Dathan N, Pedone C, Miele C, Formisano P, Beguinot F, Ruvo M
* Peptide antagonists of the PED-hPLD1 binding (344 views)
Adv Exp Med Biol (ISSN: 0065-2598, 0065-2598print, 0065-2598linking), 2009; 611: 445-446.
Impact Factor: 2.02
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Merlino A, Varriale S, Coscia MR, Mazzarella L, Oreste U
* Structure and dimerization of the teleost transmembrane immunoglobulin region (336 views)
Journal Of Molecular Graphics & Modelling (ISSN: 1093-3263), 2008 Oct; 27(3): 401-407.
Impact Factor: 2.347
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Vitagliano L, Ruggiero A, Pedone C, Berisio R
* Conformational states and association mechanism of Yersinia pestis Caf1 subunits (307 views)
Biochem Biophys Res Commun (ISSN: 0006-291x, 1090-2104, 1090-2104electronic), 2008 Sep 8; 372(4): 804-810.
Impact Factor: 2.648
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Viparelli F, Cassese A, Doti N, Paturzo F, Marasco D, Dathan NA, Monti SM, Basile G, Ungaro P, Sabatella M, Miele C, Teperino R, Consiglio E, Pedone C, Beguinot F, Formisano P, Ruvo M
* Targeting of PED/PEA-15 molecular interaction with phospholipase D1 enhances insulin sensitivity in skeletal muscle cells (411 views)
Jbc Papers (ISSN: 0021-9258, 1083-351x, 0021-9258linking), 2008 Sep 1; 283(31): 21769-21778.
Impact Factor: 5.52
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Ruvo M, Marasco D, Viparelli F, Tornatore L, Sandomenico A, Doti N, Pedone C, Benedetti E, Monti SM
* Peptide antagonists of protein-protein interactions identified by screening protein fragments derived by enzyme degradation (320 views)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2008 Sep; 14(8): N/D-N/D.
Impact Factor: 1.654
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Tornatore L, Marasco D, Dathan N, Vitale RM, Benedetti E, Papa S, Franzoso G, Ruvo M, Monti SM
* Gadd45 beta forms a homodimeric complex that binds tightly to MKK7 (431 views)
J Mol Biol (ISSN: 0022-2836, 0022-2283, 1089-8638), 2008 Apr 18; 378(1): 97-111.
Impact Factor: 5.673
View Export to BibTeX Export to EndNote
Vitagliano L, Ruggiero A, Pedone C, Berisio R
* A Molecular Dynamics Study of Pilus Subunits: Insights into Pilus Biogenesis (396 views)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2007 Apr 6; 367(4): 935-941.
Impact Factor: 4.472
View Export to BibTeX Export to EndNote
Galdiero S, Galdiero M, Pedone C
* Beta-Barrel Membrane Bacterial Proteins: Structure, Function, Assembly And Interaction With Lipids (399 views)
Curr Protein Pept Sci (ISSN: 1389-2037, 1389-2037linking), 2007 Feb; 8(1): 63-82.
Impact Factor: 3.259
View Export to BibTeX Export to EndNote
Chiechio S, Copani A, Gereau R, Nicoletti F
* Acetyl-l-carnitine in neuropathic pain (263 views)
Cns Drugs (ISSN: 1172-7047), 2007; 21: 38-38.
Impact Factor: 4.514
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37 Records (33 excluding Abstracts).
Total impact factor: 123.886 (108.811 excluding Abstracts).
Total 5 year impact factor: 136.71 (122.183 excluding Abstracts).
Total impact factor: 123.886 (108.811 excluding Abstracts).
Total 5 year impact factor: 136.71 (122.183 excluding Abstracts).
421 views. Last view on Friday 09 September 2022, 17:06:33
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