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 Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging (111 visite) Biosens Bioelectron (ISSN: 0956-5663, 1873-4235electronic), 2010 May 15; 25(9): 2095-2100. Tipo di articolo: Journal Article, Impact factor: 5.361, Impact factor a 5 anni: 5.397 Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-77951622977&partnerID=40&md5=0197784a9f4f3c1c0364eb8176933242 Parole chiave: Biosensors, Dna Sensing, Genomic Dna, Pcr-Free, Peptide Nucleic Acids, Surface-Plasmon Resonance, Biosensing, Certified Reference Materials, Detection Methods, Direct Detection, Dna Detection, Enhanced Surface, Genetically Modified, Labeling Steps, Selectivity And Sensitivity, Target Sequences, Ultrasensitive Detection, Biomolecules, Dna Sequences, Nanoparticles, Polymerase Chain Reaction, Surface Plasmon Resonance, Complementary Dna, Analytic Method, Controlled Study, Dna Determination, Gene Amplification, Gene Targeting, Image Enhancement, Sensitivity Analysis, Base Sequence, Recombinant, Gold, Metal Nanoparticles, Plants, Soybeans, Affiliazioni: *** IBB - CNR *** Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, I-95125 Catania, Italy Dipartimento di Chimica Organica ed Industriale, Università di Parma, Viale G.P. Usberti 17/a, I-43100 Parma, Italy Neotron S.p.a., Stradello Aggazzotti 104, I-41010 Modena, Italy Scuola Superiore di Catania, C/o Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, Catania, Italy Istituto Biostrutture e Bioimmagini, CNR, Viale A. Doria 6, Catania, Italy Riferimenti: Arena, G., Contino, A., Longo, E., Sgarlata, C., Spoto, G., Zito, V., (2004) Chem. Commun., 16, pp. 1812-181 Berdal, K.G., Bøydler, C., Tengs, T., Holst-Jensen, A., (2008) Eur. Food Res. Technol., 227, pp. 1149-1157 Burns, C., Spendel, W.U., Puckett, S., Pacey, G.E., (2006) Talanta, 69, pp. 873-876 D'Agata, R., Corradini, R., Grasso, G., Marchelli, R., Spoto, G., (2008) ChemBioChem, 9, pp. 2067-2070 D'Agata, R., Grasso, G., Iacono, G., Spoto, G., Vecchio, G., (2006) Org. Biomol. Chem., 4, pp. 610-612 Dos Santos Riccardi, C., Kranz, C., Kowalik, J., Yamanaka, H., Mizaikoff, B., Josowicz, M., (2008) Anal. 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Ed., 45, pp. 3303-3306 Storhoff, J.J., Lucas, A.D., Garimella, V., Bao, Y.P., Müller, U.R., (2004) Nat. Biotechnol., 22, pp. 883-887 Sujit, K.G., Tarasankar, P., (2007) Chem. Rev., 107, pp. 4797-4862 Totsingan, F., Rossi, S., Corradini, R., Tedeschi, T., Sforza, S., Juris, A., Scaravelli, E., Marchelli, R., (2008) Org. Biomol. Chem., 6, pp. 1232-1237 Wark, A.W., Lee, H.J., Corn, R.M., (2008) Angew. Chem. Int. Ed., 47, pp. 644-652 Yang, K., Moon, J.-K., Jeong, N., Back, K., Kim, H.M., Jeong, S.C., (2008) Genomics, 92, pp. 52-59 Zanoli, L., D'Agata, R., Spoto, G., (2008) Minerva Biotechnol., 20, pp. 165-174 Zhang, N., Appella, D.H., (2007) J. Am. Chem. Soc., 129, pp. 8424-8425 Zhao, W., Brook, M.A., Li, Y.F., (2008) ChemBioChem, 9, pp. 2363-2371 Zhu, D., Tang, Y., Xing, D., Chen, W.R., (2008) Anal. Chem., 80, pp. 3566-3571 Technologies today available for the DNA detection rely on a combination of labeled probes hybridized to target sequences which are amplified by polymerase chain reaction (PCR). Direct detection methods that eliminate the requirement for both PCR and labeling steps could afford faster, cheaper and simpler devices for the analysis of small amounts of unamplified DNA. In this work we describe the results obtained in the ultrasensitive detection of non-amplified genomic DNA. We analyzed certified reference materials containing different amounts of genetically modified DNA by using a detection method which combines the nanoparticle-enhanced surface plasmon resonance imaging (SPRI) biosensing to the peptide nucleic acids (PNAs) improved selectivity and sensitivity in targeting complementary DNA sequences. The method allowed us to obtain a 41 zM sensitivity in targeting genomic DNA even in the presence of a large excess of non-complementary DNA. (C) 2010 Elsevier B.V. All rights reserved.
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Impact Factor: 0.723 Dettagli Esporta in BibTeX Esporta in EndNote Roviello GN, Moccia M, Sapio R, Valente M, Bucci EM, Castiglione M, Pedone C, Perretta G, Benedetti E, Musumeci D * Synthesis, Characterization And Hybridization Studies Of New Nucleo-Gamma-Peptides Based On Diaminobutyric Acid (138 visite) J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2006 Dec; 12(12): 829-835. Impact Factor: 1.801 Dettagli Esporta in BibTeX Esporta in EndNote Borgatti M, Boyd DD, Lampronti I, Bianchi N, Fabbri E, Saviano M, Romanelli A, Pedone C, Gambari R * Decoy molecules based on PNA-DNA chimeras and targeting Sp1 transcription factors inhibit the activity of urokinase-type plasminogen activator receptor (uPAR) promoter (126 visite) Oncology Research (ISSN: 0965-0407), 2005; 15(7-8): 373-383. Impact Factor: 1.844 Dettagli Esporta in BibTeX Esporta in EndNote Borgatti M, Finotti A, Romanelli A, Saviano M, Bianchi N, Lampronti I, Lambertini E, Penolazzi L, Nastruzzi C, Mischiati C, Piva R, Pedone C, Gambari R * Peptide nucleic acids (PNA)-DNA chimeras targeting transcription factors as a tool to modify gene expression (132 visite) Curr Drug Targets (ISSN: 1389-4501), 2004 Nov; 5(8): 735-744. Impact Factor: 4.104 Dettagli Esporta in BibTeX Esporta in EndNote Cortesi R, Mischiati C, Borgatti M, Breda L, Romanelli A, Saviano M, Pedone C, Gambari R, Nastruzzi C * Formulations for natural and peptide nucleic acids based on cationic polymeric submicron particles (98 visite) The Aaps Journal (ISSN: 1522-1059, 1550-7416), 2004 Jan 20; 6(1): N/D-N/D. Impact Factor: 1.938 Dettagli Esporta in BibTeX Esporta in EndNote Musumeci D, Roviello GN, Valente M, Sapio R, Pedone C, Bucci EM * New synthesis of PNA-3′DNA linker monomers, useful building blocks to obtain PNA/DNA chimeras (91 visite) Biopolymers, 2004; 76(6): 535-542. Impact Factor: 2.248 Dettagli Esporta in BibTeX Esporta in EndNote Gambari R, Borgatti M, Romanelli A, Capasso D, Saviano M, Bianchi N, Lampronti I, Mischiati C, Pedone C * Transcription factor decoy (TFD) activity of peptide nucleic acids (PNA)-DNA chimeras (93 visite) International Journal Of Molecular Medicine (ISSN: 1107-3756), 2003; 12(Supplement1): N/D-N/D. Impact Factor: 1.94 Dettagli Esporta in BibTeX Esporta in EndNote Romanelli A, Pedone C, Saviano M, Bianchi N, Borgatti M, Mischiati C, Gambari R * Molecular interactions between nuclear factor κB (NF-κB) transcription factors and a PNA-DNA chimera mimicking NF-κB binding sites (105 visite) Eur J Biochem (ISSN: 0014-2956, 0014-2956print, 0014-2956linking), 2001 Dec; 268(23): 6066-6075. Impact Factor: 2.849 Dettagli Esporta in BibTeX Esporta in EndNote Romanelli A, Pedone C, Saviano M, Bianchi N, Borgatti M, Mischiati C, Gambari R * Molecular interactions with nuclear factor kappaB (NF-kappaB) transcription factors of a PNA-DNA chimera mimicking NF-kappaB binding sites (96 visite) Eur J Biochem (ISSN: 0014-2956print, 0014-2956linking), 2001 Dec; 268(23): 6066-6075. Impact Factor: 4.779 Dettagli Esporta in BibTeX Esporta in EndNote 47 Records (39 escludendo Abstract e Conferenze). Impact factor totale: 120.433 (108.29 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 122.458 (107.901 escludendo Abstract e Conferenze). Esporta in BibTeX Esporta in EndNote Ultima modifica di Marco Comerci in data Thursday 19 March 2015, 19:54:49 111 visite. Ultima visita in data Sunday 30 December 2018, 6:25:19 |
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 Post Doctoral fellowship w2019 award Fondazione Umberto Veronesi  Innova S@lute 2017Bambini Liberi progetto coordinato dal Cnr, vince il premio Innova S@lute 2017 (161 visite)  Sperimentato su pazienti pediatrici un esoscheletro personalizzato progettato da ricercatori Cnr (485 visite)SmartCity Radio24-Sole 24 Ore Intervista il Dott. Alessandro Soluri sulla Pocket Gamma Camera (272 visite) Accordo cooperazione tra IBB ed il Dipartimento di Ingegneria Biomedica dell’Università di Pechino (567 visite) Archivio Notizie>>> (766 visite) Post Doctoral fellowship w2019 award Fondazione Umberto Veronesi Innova S@lute 2017Bambini Liberi progetto coordinato dal Cnr, vince il premio Innova S@lute 2017 (161 visite) Sperimentato su pazienti pediatrici un esoscheletro personalizzato progettato da ricercatori Cnr (485 visite)SmartCity Radio24-Sole 24 Ore Intervista il Dott. Alessandro Soluri sulla Pocket Gamma Camera (272 visite) Accordo cooperazione tra IBB ed il Dipartimento di Ingegneria Biomedica dell’Università di Pechino (567 visite) Archivio Notizie>>> (766 visite) Post Doctoral fellowship w2019 award Fondazione Umberto Veronesi Innova S@lute 2017Bambini Liberi progetto coordinato dal Cnr, vince il premio Innova S@lute 2017 (161 visite) Sperimentato su pazienti pediatrici un esoscheletro personalizzato progettato da ricercatori Cnr (485 visite)SmartCity Radio24-Sole 24 Ore Intervista il Dott. Alessandro Soluri sulla Pocket Gamma Camera (272 visite) Accordo cooperazione tra IBB ed il Dipartimento di Ingegneria Biomedica dell’Università di Pechino (567 visite) Archivio Notizie>>> (766 visite) Concorsi
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