Peptide receptor radionuclide therapy (PRRT) has recently expanded due to radiolabelling of DOTA-peptides, such as the somatostatin analogues [DOTA0, Tyr3]octreotate (DOTATATE).The achievement of high specific activities during procedures has been indicated as the critical factor to consent effective therapy. Several radiochemical factors may negatively impact reaction procedures such as pH, temperature and time of reaction. Our study was undertaken to explore the influence of radiochemical parameters, such as time of incubation, on reaction kinetics during the radiolabelling of DOTATATE with 90Y. Methods: Forty-five radiolabelling procedures were carried out using small volumes of yttrium-90, typically 60-78 γL. At nearly constant pH and temperature two different settings of radiolabelling procedures were implemented, removing the products from the heating water bath approximately after 30. min (group E, early; n=20) and after 39. min (group L, later; n=25). Quality controls were performed by means of both high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). Results: Reaction kinetics for 90Y were found to a provide suitable percentage of incorporation at pH 4.5 for both groups. Reaction temperature was not different between groups E and L. A significant difference was found between the two groups in radiochemical yield, which was 95.6%±0.8 for group E and 98.2%±1.1 for group L (p<0.0001). The specific activity of the final product was 46.9MBq/nmol. Conclusion: In order to achieve optimal specific activities, pH, temperature and time of reaction necessitate careful evaluation and setting. A statistically significant difference in labelling yield was found between a set of procedures completed at 39. min as compared to that executed at 30. min, keep the reaction pH and temperature constant. © 2010 Elsevier Ltd.