Crystallization and preliminary X-ray analysis of the complex of human alpha-thrombin with a modified thrombin-binding aptamer(288 views visite) Russo Krauss I, Merlino A, Randazzo A, Mazzarella L, Sica F
Keywords Parole chiave: Protein-Nucleic Acid Interactions, Quadruplexes, Thrombin, Thrombin-Binding Aptamers, Thrombin Aptamer, Article, Chemistry, Crystallization, Human, Metabolism, Protein Binding, Protein Multimerization, X Ray Crystallography, Nucleotide, X-Ray, Base Sequence, Biomimetic Materials, Chemical Synthesis, Chemistry Techniques, Synthetic, Catalytic, Genetics, 10-23, Chimeric Oligonucleotide, Dnazyme, Deoxyribozyme, Nucleic Acid Crystallization,
Affiliations Affiliazioni: *** IBB - CNR ***
Dipartimento di Chimica, Universit di Napoli Federico II, Via Cintia, I-80126 Napoli, Italy Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, I-80134 Napoli, Italy a Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences , Department of Bioorganic Chemistry , Lodz , Poland.,
References Riferimenti: Bock, L. C., Griffin, L. C., Latham, J. A., Vermaas, E. H., Toole, J. J., (1992) Nature (London), 355, pp. 564-56
Bode, W., Mayr, I., Baumann, U., Huber, R., Stone, S. R., Hofsteenge, J., (1989) EMBO J., 8, pp. 3467-3475
Kelly, J. A., Feigon, J., Yeates, T. O., (1996) J. Mol. Biol., 256, pp. 417-422
Lancellotti, S., De Cristofaro, R., (2009) Cardiovasc. Hematol. Agents Med. Chem., 7, pp. 19-28
MacAya, R. F., Schultze, P., Smith, F. W., Roe, J. A., Feigon, J., (1993) Proc. Natl Acad. Sci. USA, 90, pp. 3745-3749
Martino, L., Virno, A., Randazzo, A., Virgilio, A., Esposito, V., Giancola, C., Bucci, M., Mayol, L., (2006) Nucleic Acids Res., 34, pp. 6653-6662
Navaza, J., Saludjian, P., (1997) Methods Enzymol., 276, pp. 581-594
Otwinowski, Z., Minor, W., (1997) Methods Enzymol., 276, pp. 307-326
Padmanabhan, K., Padmanabhan, K. P., Ferrara, J. D., Sadler, J. E., Tulinsky, A., (1993) J. Biol. Chem., 268, pp. 17651-17654
Padmanabhan, K., Tulinsky, A., (1996) Acta Cryst., D52, pp. 272-282
Pagano, B., Martino, L., Randazzo, A., Giancola, C., (2007) Biophys. J., 94, pp. 562-569
Schultze, P., MacAya, R. F., Feigon, J., (1994) J. Mol. Biol., 235, pp. 1532-1547
Crystallization and preliminary X-ray analysis of the complex of human alpha-thrombin with a modified thrombin-binding aptamer
The thrombin-binding aptamer (TBA) is a consensus DNA 15-mer that binds specifically to human-thrombin at nanomolar concentrations and inhibits its procoagulant functions. Recently, a modified TBA (mTBA) containing a 5 -5 inversion-of-polarity site has been shown to be more stable and to possess a higher thrombin affinity than its unmodified counterpart. The structure of the thrombin-TBA complex has previously been determined at low resolution, but did not provide a detailed picture of the aptamer conformation or of the protein-DNA assembly, while that of the complex with mTBA is unknown. Crystallographic analysis of the thrombin-mTBA complex has been attempted. The crystals diffracted to 2. 15 resolution and belonged to space group I222. 2010 International Union of Crystallography All rights reserved
Crystallization and preliminary X-ray analysis of the complex of human alpha-thrombin with a modified thrombin-binding aptamer
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L * Combined HAT/EZH2 modulation leads to cancer-selective cell death(199 visite) Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646. Impact Factor:5.008 DettagliEsporta in BibTeXEsporta in EndNote
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ * Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer(211 visite) N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756. Impact Factor:59.558 DettagliEsporta in BibTeXEsporta in EndNote
Santulli G, Cipolletta E, Sorriento D, Del Giudice C, Anastasio A, Monaco S, Maione AS, Condorelli G, Puca A, Trimarco B, Illario M, Iaccarino G * CaMK4 gene deletion induces hypertension(277 visite) J Am Heart Assoc Journal Of The American Heart Association (ISSN: 2047-9980), 2012; 1(4): N/D-N/D. Impact Factor:2.882 DettagliEsporta in BibTeXEsporta in EndNote
Antonini A, Vitale C, Barone P, Cilia R, Righini A, Bonuccelli U, Abbruzzese G, Ramat S, Petrone A, Quatrale R, Marconi R, Ceravolo R, Stefani A, Lopiano L, Zappia M, Capus L, Morgante L, Tamma F, Tinazzi M, Colosimo C, Guerra UP, Valzania F, Fagioli G, Distefano A, Bagnato A, Feggi L, Anna S, Maria Teresa Rosaria De Cr, Nobili F, Mazzuca N, Baldari S, Eleopra R, Bestetti A, Benti R, Varrone A, Volterrani D, Massa R, Stocchi F, Schillaci O, Dore F, Zibetti M, Castellano G, Battista SG, Giorgetti G * The relationship between cerebral vascular disease and parkinsonism: The VADO study(406 visite) Parkinsonism Relat D (ISSN: 1353-8020, 1873-5126, 1873-5126electronic), 2012; 18(6): 775-780. Impact Factor:3.274 DettagliEsporta in BibTeXEsporta in EndNote
Malvindi MA, Greco A, Conversano F, Figuerola A, Corti M, Bonora M, Lascialfari A, Doumari HA, Moscardini M, Cingolani R, Gigli G, Casciaro S, Pellegrino T, Ragusa A * MR Contrast Agents(224 visite) Small Animal Imaging, 2011 Jul 8; 21(13): 2548-2555. Impact Factor:1.784 DettagliEsporta in BibTeXEsporta in EndNote