Formulations for natural and peptide nucleic acids based on cationic polymeric submicron particles(242 visite) Cortesi R, Mischiati C, Borgatti M, Breda L, Romanelli A, Saviano M, Pedone C, Gambari R, Nastruzzi C
The Aaps Journal (ISSN: 1522-1059, 1550-7416), 2004 Jan 20; 6(1): N/D-N/D.
Tipo di articolo: Journal Article, Abstract, Conference,
Impact factor: 1.938, Impact factor a 5 anni: 4.97
Parole chiave: Acrylic Acid Resin, Cation, Didodecyldimethylammonium, Drug Carrier, Eudragit Rs, Luciferase, Nucleic Acid, Peptide Nucleic Acid, Quaternary Ammonium Derivative, Surfactant, Adenocarcinoma, Animal, Article, Biosynthesis, Breast Tumor, Cell Culture, Chemistry, Colloid, Drug Stability, Female, Genetic Transfection, Human, Macrophage, Methodology, Mouse, Particle Size, Pathology, Reporter Gene, Tumor Cell Line, Acrylic Resins, Breast Neoplasms, Quaternary Ammonium Compounds, Surface-Active Agents, Delivery, Submicron Particles, Benzethonium Chloride, Cationic Surfactant, Domiphen Bromide, Lipofectamine, Liposome, Polymer, Animal Cell, Cell Growth, Cell Interaction, Cell Proliferation, Cell Strain K 562, Complex Formation, Controlled Study, Cytotoxicity, Drug Delivery System, Drug Formulation, Drug Storage, Drug Synthesis, Drug Transport, Electron Microscopy, Enzyme Activity, Enzyme Assay, Gene Targeting, Human Cell, In Vitro Study, Intermethod Comparison, Nonhuman, Physical Chemistry, Scanning Electron Microscopy, Spray Drying, Zeta Potential,
*** IBB - CNR *** Department of Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy. Dept. of Biochemistry/Molec. Biology, University of Ferrara, Ferrara, Italy Institute of Biostructure/Bioimaging, CNR, Napoli, France Lab. Devmt. Pharmacol./Pharmacogen., Biotechnology Centre, University of Ferrara, Ferrara, Italy Dept. of Pharmaceut. Chem./Technol., University of Perugia, Perugia, Italy
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This article describes the production and characterization of cationic submicron particles constituted with Eudragit RS 100, plus different cationic surfactants, such as dioctadecyl-dimethyl-ammonium bromide (DDAB18) and diisobutyphenoxyethyl-dimethylbenzyl ammonium chloride (DEBDA), as a transport and delivery system for DNA/DNA and DNA/peptide nucleic acid (PNA) hybrids and PNA-DNA chimeras. Submicron particles could offer advantages over other delivery systems because they maintain unaltered physicochemical properties for long time periods, allowing long-term storage, and are suitable for industrial production. Submicron particles were characterized in terms of size, size distribution, morphology, and zeta potential. Moreover, the in vitro activity and ability of submicron particles to complex different types of nucleic acids were described. Finally, the ability of submicron particles to deliver functional genes to cells cultured in vitro was determined by a luciferase activity assay, demonstrating that submicron particles possess superior transfection efficiency with respect to commercially available, liposome-based transfection kits.
140 Records (132 escludendo Abstract e Conferenze). Impact factor totale: 614.293 (597.194 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 665.972 (646.703 escludendo Abstract e Conferenze).