Formulations for natural and peptide nucleic acids based on cationic polymeric submicron particles(198 visite) Cortesi R, Mischiati C, Borgatti M, Breda L, Romanelli A, Saviano M, Pedone C, Gambari R, Nastruzzi C
The Aaps Journal (ISSN: 1522-1059, 1550-7416), 2004 Jan 20; 6(1): N/D-N/D.
Tipo di articolo: Journal Article, Abstract, Conference,
Impact factor: 1.938, Impact factor a 5 anni: 4.97
Parole chiave: Acrylic Acid Resin, Cation, Didodecyldimethylammonium, Drug Carrier, Eudragit Rs, Luciferase, Nucleic Acid, Peptide Nucleic Acid, Quaternary Ammonium Derivative, Surfactant, Adenocarcinoma, Animal, Article, Biosynthesis, Breast Tumor, Cell Culture, Chemistry, Colloid, Drug Stability, Female, Genetic Transfection, Human, Macrophage, Methodology, Mouse, Particle Size, Pathology, Reporter Gene, Tumor Cell Line, Acrylic Resins, Breast Neoplasms, Quaternary Ammonium Compounds, Surface-Active Agents, Delivery, Submicron Particles, Benzethonium Chloride, Cationic Surfactant, Domiphen Bromide, Lipofectamine, Liposome, Polymer, Animal Cell, Cell Growth, Cell Interaction, Cell Proliferation, Cell Strain K 562, Complex Formation, Controlled Study, Cytotoxicity, Drug Delivery System, Drug Formulation, Drug Storage, Drug Synthesis, Drug Transport, Electron Microscopy, Enzyme Activity, Enzyme Assay, Gene Targeting, Human Cell, In Vitro Study, Intermethod Comparison, Nonhuman, Physical Chemistry, Scanning Electron Microscopy, Spray Drying, Zeta Potential,
*** IBB - CNR *** Department of Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy. Dept. of Biochemistry/Molec. Biology, University of Ferrara, Ferrara, Italy Institute of Biostructure/Bioimaging, CNR, Napoli, France Lab. Devmt. Pharmacol./Pharmacogen., Biotechnology Centre, University of Ferrara, Ferrara, Italy Dept. of Pharmaceut. Chem./Technol., University of Perugia, Perugia, Italy
Nielsen, P.E., Egholm, M., Berg, R.H., Buchardt, O., Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide (1991) Science, 254, pp. 1497-150
Egholm, M., Buchardt, O., Nielsen, P.E., Berg, R.H., Peptide nucleic acids (PNA): Oligonucleotide analogues with an achiral peptide backbone (1992) J. Am. Chem. Soc., 114, pp. 1895-1897
Gambari, R., Peptide nucleic acids (PNAs): A tool for the development of gene expression modifiers (2001) Curr. Pharm. Design, 7, pp. 1839-1862
Demidov, V.V., Potaman, V.N., Frank-Kamenetsk, M.D., Stability of peptide nucleic acids in human serum and cellular extracts (1994) Biochem. Pharmacol., 48, pp. 1310-1313
Scarfì, S., Giovine, M., Gasparini, A., Modified peptide nucleic acids are internalized in mouse macrophages RAW 264.7 and inhibit inducible nitric oxide synthase (1999) FEBS Lett., 451, pp. 264-268
Nastruzzi, C., Cortesi, R., Esposito, E., Liposomes as carriers for DNA-PNA hybrids (2000) J. Control Release, 68, pp. 237-249
Vinayak, R., Van der Laan, A.C., Brill, R., Automated chemical synthesis of PNA and PNA-DNA chimera on a nucleic acid synthesizer (1997) Nucleosides Nucleotides, 16, pp. 1653-1658
Romanelli, E., Pedone, C., Saviano, M., Molecular interactions between nuclear factor kB (NF-kB) transcription factors and a PNA-DNA chimera mimicking NF-kB binding sites (2001) Eur. J. Biochem., 268, pp. 1-11
Borgatti, M., Breda, L., Cortesi, R., Cationic liposomes as delivery systems for double-stranded peptide nucleic acid (PNA)-DNA chimeras exhibiting decoy activity against nuclear factor-kB transcription factors (2002) Biochem. Pharmacol., 64, pp. 609-616
Fundueanu, G., Mocanu, G., Constantin, M., Acrylic microspheres for oral controlled release of the biguanide buformin (2001) Int. J. Pharm., 218, pp. 13-25
Esposito, E., Roncarati, R., Cortesi, R., Cervellati, F., Nastruzzi, C., Production of Eudragit microparticles by spray-drying technique: Influence of experimental parameters on morphological and dimensional characteristics (2000) Pharm. Dev. Technol., 5, pp. 267-278
Esposito, E., Sebben, S., Cortesi, R., Menegatti, E., Nastruzzi, C., Preparation and characterization of cationic microspheres for gene delivery (1999) Int. J. Pharm., 189, pp. 29-41
Zambaux, E., Faivre-Fiorina, B., Bonneaux, F., Involvement of neutrophilic granulocytes in the uptake of biodegradable non-stealth and stealth nanoparticles in guinea pig (2000) Biomaterials, 21, pp. 975-980
Fernandez-Urrusuno, R., Fattal, E., Rodrigues Jr., J.M., FeH ger, J., Bedossa, P., Couvreur, P., Effect of polymeric nanoparticle administration on the clearance activity of the mononuclear phagocyte system in mice (1996) J. Biomed. Mater. Res., 31, pp. 401-408
Esposito, E., Cortesi, R., Porta, R., Trento, F., Nastruzzi, C., Effect of long-term stabilization of cationic liposomes as defibrotide delivery system for antithrombotic activity (2002) Drug Dev. Res., 5, pp. 127-138
This article describes the production and characterization of cationic submicron particles constituted with Eudragit RS 100, plus different cationic surfactants, such as dioctadecyl-dimethyl-ammonium bromide (DDAB18) and diisobutyphenoxyethyl-dimethylbenzyl ammonium chloride (DEBDA), as a transport and delivery system for DNA/DNA and DNA/peptide nucleic acid (PNA) hybrids and PNA-DNA chimeras. Submicron particles could offer advantages over other delivery systems because they maintain unaltered physicochemical properties for long time periods, allowing long-term storage, and are suitable for industrial production. Submicron particles were characterized in terms of size, size distribution, morphology, and zeta potential. Moreover, the in vitro activity and ability of submicron particles to complex different types of nucleic acids were described. Finally, the ability of submicron particles to deliver functional genes to cells cultured in vitro was determined by a luciferase activity assay, demonstrating that submicron particles possess superior transfection efficiency with respect to commercially available, liposome-based transfection kits.
139 Records (131 escludendo Abstract e Conferenze). Impact factor totale: 608.817 (591.718 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 660.495 (641.226 escludendo Abstract e Conferenze).