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Probing the activity of diguanylate cyclases and c-di-GMP phosphodiesterases in real-time by CD spectroscopy (133 visite)

Stelitano V, Brandt A, Fernicola S, Franceschini S, Giardina G, Pica A, Rinaldo S, Sica F, Cutruzzola F

Nucleic Acids Res (ISSN: 0305-1048, 0305-5104, 1362-4962), 2013 Apr; 41(7): e79-e79.

Tipo di articolo: Journal Article, Abstract, Conference, Research Support, Non-U. S. Gov'T, , Impact factor: 4.188, Impact factor a 5 anni: 7.163, Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84876543297&partnerID=40&md5=de1331fe5198c9ad7a72605c1a4cb7aa

Parole chiave: Cyclic Diguanylic Acid, Cyclic Adenilic Acid, Cyclic Gmp Phosphodiesterase, Diguanylate Cyclase, Dimer, Guanine, Manganese, Unclassified Drug, Article, Biofilm, Catalysis, Circular Dichroism, Concentration (parameters), Controlled Study, Intercalation Complex, Nonhuman, Priority Journal, Ultraviolet Radiation, -Cyclic-Gmp Phosphodiesterases, Models, Molecular, Metabolism, Methods, Analogs, Derivatives, Chemistry,

Affiliazioni: *** IBB - CNR ***
Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Sciences, Sapienza University of Rome, 00185 Rome, Italy
Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy
Institute of Biostructure and Bioimaging, CNR, 80134 Naples, Italy


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Bacteria react to adverse environmental stimuli by clustering into organized communities called biofilms. A remarkably sophisticated control system based on the dinucleotide 3 -5 cyclic diguanylic acid (c-di-GMP) is involved in deciding whether to form or abandon biofilms. The ability of c-di-GMP to form self-intercalated dimers is also thought to play a role in this complex regulation. A great advantage in the quest of elucidating the catalytic properties of the enzymes involved in c-di-GMP turnover (diguanylate cyclases and phosphodiesterases) would come from the availability of an experimental approach for in vitro quantification of c-di-GMP in real-time. Here, we show that c-di-GMP can be detected and quantified by circular dichroism (CD) spectroscopy in the low micromolar range. The method is based on the selective ability of manganese ions to induce formation of the intercalated dimer of the c-di-GMP dinucleotide in solution, which displays an intense sigmoidal CD spectrum in the near-ultraviolet region. This characteristic spectrum originates from the stacking interaction of the four mutually intercalated guanines, as it is absent in the other cyclic dinucleotide 3 -5 cyclic adenilic acid (c-di-AMP). Thus, near-ultraviolet CD can be used to effectively quantify in real-time the activity of diguanylate cyclases and phosphodiesterases in solution. 2013 The Author (s)
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Puglisi A, Tabbì G, Vecchio G
* Bioconjugates of cyclodextrins of manganese salen-type ligand with superoxide dismutase activity (87 visite)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2004 Jun; 98(6): 969-976.
Impact Factor: 2.225
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1 Records (1 escludendo Abstract e Conferenze).
Impact factor totale: 2.225 (2.225 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 3.452 (3.452 escludendo Abstract e Conferenze).







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