Home Ricerca Sedi Chi siamo Organigramma Personale Contatti Didattica Login Documenti EN
Progetti in evidenza
INCIPIT
(Link esterno)

ISTAPCA
(1128 visite)

Fondazione Veronesi
(Link esterno)

Euro-BioImaging
(Link esterno)

Fondo Europeo Pesca
(554 visite)

LightDyNAmics
(1006 visite)

 INMiND
(1132 visite)

Instruct-IT
(Link esterno)

 PRIN
(1271 visite)

 eHealthNet
(1038 visite)

Ponrec
(984 visite)

MFAG Grant
(1068 visite)

MERIT
(Link esterno)


Collegamenti



Adenoviral gene transfer of Akt enhances myocardial contractility and intracellular calcium handling (112 visite)

Cittadini A, Monti MG, Iaccarino G, Di Rella F, Tsichlis PN, Di Gianni A, Strömer H, Sorriento D, Peschle C, Trimarco B, Saccà L, Condorelli G

Gene Ther Gene Therapy (ISSN: 0969-7128), 2006; 13(1): 8-19.

Tipo di articolo: Journal Article,

Impact factor: 4.782

Impact factor a 5 anni: 3.67


Parole chiave: Akt, Calcium, Heart, Oxygen Consumption, Adenosine Triphosphatase (calcium), Adenovirus Vector, Adenylate Cyclase, Aequorin, Beta Adrenergic Receptor, Buffer, Calsequestrin, Dobutamine, Messenger Rna, Phospholamban, Protein Kinase B, Ryanodine Receptor, Animal Experiment, Animal Tissue, Aorta Clamping, Article, Binding Affinity, Body Weight, Calcium Cell Level, Calcium Transport, Catheter, Cell Energy, Controlled Study, Documentation, Enzyme Activity, Gene Transfer, Heart Hypertrophy, Heart Left Ventricle, Heart Left Ventricle Contractility, Heart Left Ventricle Mass, Heart Muscle Contractility, Heart Muscle Oxygen Consumption, Heart Perfusion, Heart Ventricle Pressure, Inotropism, Isolated Heart, Myofilament, Nonhuman, Postoperative Period, Pressure Gradient, Priority Journal, Protein Content, Rat Strain, Receptor Density, Sarcoplasmic Reticulum, Steady State, Systole, Tetany, Titrimetry, Viral Gene Delivery System, Wild Type, Adenoviridae, Adrenergic Beta-Agonists, Calcium-Transporting Atpases, Echocardiography, Gene Therapy, Genetic Vectors, Heart Failure, Congestive, Myocardial Contraction, Myocardial Reperfusion Injury, Ng-Nitroarginine Methyl Ester, Nitric Oxide Synthase, Proto-Oncogene Proteins C-Akt, Sprague-Dawley, Transduction,

Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-29244470231&partnerID=40&md5=2d35430a993bef9415a2b220a3bf1830

The serine-threonine kinase Akt/PKB mediates stimuli from different classes of cardiomyocyte receptors, including the growth hormone/insulin like growth factor and the β-adrenergic receptors. Whereas the growth-promoting and antiapoptotic properties of Akt activation are well established, little is known about the effects of Akt on myocardial contractility, intracellular calcium (Ca2+) handling, oxygen consumption, and β-adrenergic pathway. To this aim, Sprague -Dawley rats were subjected to a wild-type Akt in vivo adenoviral gene transfer using a catheter-based technique combined with aortopulmonary crossclamping. Left ventricular (LV) contractility and intracellular Ca2+ handling were evaluated in an isolated isovolumic buffer-perfused, aequorin-loaded whole heart preparations 10 days after the surgery. The Ca2+-force relationship was obtained under steady-state conditions in tetanized muscles. No significant hypertrophy was detected in adenovirus with wild-type Akt (Ad.Akt) versus controls rats (LV-to-body weight ratio 2.6±177;0.2 versus 2.7±177;0.1mg/g, controls versus Ad.Akt, P, NS). LV contractility, measured as developed pressure, increased by 41% in Ad.Akt. This was accounted for by both more systolic Ca2+ available to the contractile machinery (+19% versus controls) and by enhanced myofilament Ca2+ responsiveness, documented by an increased maximal Ca2+-activated pressure (+19% versus controls) and a shift to the left of the Ca2+-force relationship. Such increased contractility was paralleled by a slight increase of myocardial oxygen consumption (14%), while titrated dose of dobutamine providing similar inotropic effect augmented oxygen consumption by 39% (P
*** IBB - CNR ***

Department of Clinical Medicine and Cardiovascular Sciences, University 'Federico II', Via S Pansini 5, Naples 80131, Italy

Molecular Oncology Research Institute, New England Medical Center, Boston, MA, United States

Medizinische Universitätsklinik, Würzburg, Germany

Instituto Superiore Sanita', Rome, Italy

San Raffaele Biomedical Science Park, Rome-Multimedica Hospital, Milan, Italy

Institute of Molecular Medicine, University of California, San Diego, CA, United States
Testa, J.R., Bellacosa, A., Akt plays a central role in tumorigenesis (2001) Proc Natl Acad Sci USA, 98, pp. 10983-1098

Oh, H., Fujio, Y., Kunisada, K., Hirota, H., Matsui, H., Kishimoto, T., Activation of phosphatidylinositol 3-kinase through glycoprotein 130 induces protein kinase B and p70 S6 kinase phosphorylation in cardiac myocytes (1998) J Biol Chem, 273, pp. 9703-9710

Morisco, C., Zebrowski, D.C., Vatner, D.E., Vatner, S.F., Sadoshima, J., The Akt-glycogen synthase kinase 3beta pathway regulates transcription of atrial natriuretic factor induced by beta-adrenergic receptor stimulation in cardiac myocytes (2000) J Biol Chem, 275, pp. 14466-14475

Matsui, T., Nagoshi, T., Rosenzweig, A., Akt and PI 3-kinase signaling in cardiomyocyte hypertrophy and survival (2003) Cell Cycle, 2, pp. 220-223

Shioi, T., McMullen, J.R., Kang, P.M., Douglas, P.S., Obata, T., Franke, T.F., Akt/protein kinase B promotes organ growth in transgenic mice (2002) Mol Cell Biol, 22, pp. 2799-2809

Yamashita, K., Kajstura, J., Discher, D.J., Wasserlauf, B.J., Bishopric, N.H., Anversa, P., Reperfusion-activated Akt kinase prevents apoptosis in transgenic mouse hearts overexpressing insulin-like growth factor-1 (2001) Circ Res, 88, pp. 609-614

Miao, W., Luo, Z., Kitsis, R.N., Walsh, K., Intracoronary adenovirus-mediated Akt gene transfer in heart limits infarct size following ischemia-reperfusion injury in vivo (2000) J Mol Cell Cardiol, 32, pp. 2397-2402

Matsui, T., Tao, J., del Monte, F., Lee, K.H., Li, L., Picard, M., Akt preserves cardiac function and prevents injury after transient cardiac ischemia in vivo (2001) Circulation, 104, pp. 330-335

Fujio, Y., Nguyen, T., Wencker, D., Kitsis, R.N., Walsh, K., Akt promotes survival of cardiomyocytes in vitro and protects against ischemia-reperfusion injury in mouse heart (2000) Circulation, 101, pp. 660-667

Condorelli, G., Drusco, A., Stassi, G., Bellacosa, A., Roncarati, R., Iaccarino, G., Akt induces enhanced myocardial contractility and cell size in vivo in transgenic mice (2002) Proc Natl Acad Sci USA, 99, pp. 12333-12338

Kim, Y.K., Kim, S.J., Yatani, A., Huang, Y., Castelli, G., Vatner, D.E., Mechanisms of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt (2003) J Biol Chem, 278, pp. 47622-47628

Bonci, D., Cittadini, A., Latronico, M.V., Borello, U., Aycock, J.K., Drusco, A., 'Advanced' generation lentiviruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo (2003) Gene Therapy, 10, pp. 630-636

Hajjar, R.J., Schmidt, U., Matsui, T., Guerrero, J.L., Lee, K.H., Gwathmey, J.K., Modulation of ventricular function through gene transfer in vivo (1998) Proc Natl Acad Sci USA, 95, pp. 5251-5256

Ikeda, Y., Gu, Y., Iwanaga, Y., Hoshijima, M., Oh, S.S., Giordano, F.J., Restoration of deficient membrane proteins in the cardiomyopathic hamster by in vivo cardiac gene transfer (2002) Circulation, 105, pp. 502-508

Camacho, S.A., Brandes, R., Figueredo, V.M., Weiner, M.W., Ca2+ transient decline and myocardial relaxation are slowed during low flow ischemia in rat hearts (1994) J Clin Invest, 93, pp. 951-957

Blinks, J.R., Endoh, M., Modification of myofibrillar responsiveness to Ca2+ as an inotropic mechanism (1986) Circulation, 73, pp. III85-III98

Perrault, C.L., Meuse, A.J., Bentivegna, L.A., Morgan, J.P., Abnormal intracellular calcium handling in acute and chronic heart failure: Role in systolic and diastolic dysfunction (1990) Eur Heart J, 11, pp. 8-21

Wisloff, U., Loennechen, J.P., Falck, G., Beisvag, V., Currie, S., Smith, G., Increased contractility and calcium sensitivity in cardiac myocytes isolated from endurance trained rats (2001) Cardiovasc Res, 50, pp. 495-508

Beanlands, R.S., Bach, D.S., Raylman, R., Armstrong, W.F., Wilson, V., Montieth, M., Acute effects of dobutamine on myocardial oxygen consumption and cardiac efficiency measure using carbon-11 acetate kinetics in patients with dilated cardiomyopathy (1993) J Am Coll, 22, pp. 1389-1398

Hasenfuss, G., Holubarsch, C., Heiss, H.W., Meinertz, T., Bonzel, T., Wais, U., Myocardial energetics in patients with dilated cardiomyopathy: Influence of nitroprusside and enoximone (1989) Circulation, 80, pp. 51-64

Saccà, L., Cittadini, A., Fazio, S., Growth hormone and the heart (1994) Endocr Rev, 15, pp. 555-573

Cittadini, A., Ishiguro, Y., Stromer, H., Spindler, M., Moses, A.C., Clark, R., Insulin-like growth factor-1 but not growth hormone augments mammalian myocardial contractility by sensitizing the myofilament to Ca2+ through a wortmannin-sensitive pathway. Studies in rat and ferret isolated muscles (1998) Circ Res, 83, pp. 50-59

Tajima, M., Weinberg, E.O., Bartunek, J., Jin, H., Yang, R., Paoni, N.F., Treatment with growth hormone enhances contractile reserve and intracellular calcium transients in myocytes from rats with postinfarction heart failure (1999) Circulation, 99, pp. 127-134

He, H., Giordano, F.J., Hilal-Dandan, R., Choi, D.J., Rockman, H.A., McDonough, P.M., Overexpression of the rat sarcoplasmic reticulum Ca2+-ATPase gene in the heart of transgenic mice accelerates calcium transients and cardiac relaxation (1997) J Clin Invest, 100, pp. 380-389

Baker, D.L., Hashimoto, K., Grupp, I.L., Ji, Y., Reed, T., Loukianov, E., Targeted overexpression of the sarcoplasmic reticulum Ca2+-ATPase increases cardiac contractility in transgenic mouse hearts (1998) Circ Res, 83, pp. 1205-1214

Duan, J., Zhang, H.Y., Adkins, S.D., Ren, B.H., Norby, F.L., Zhang, X., Impaired cardiac function and IGF-I response in myocyte from calmodulin-diabetic mice: Role of Akt and RhoA (2003) Am J Physiol, 284, pp. E366-E376

Haq, S., Choukroun, G., Lim, H., Tymitz, K.M., del Monte, F., Gwathmey, J., Differential activation of signal transduction pathways in human hearts with hypertrophy versus advanced heart failure (2001) Circulation, 103, pp. 670-677

Taniyama, Y., Walsh, K., Elevated myocardial Akt signaling ameliorates doxorubicin-induced congestive heart failure and promotes heart growth (2002) J Moll Cell Cardiol, 34, pp. 1241-1247

Iaccarino, G., Ciccarelli, M., Sorriento, D., Cipolletta, E., Cerullo, V., Iovino, G.L., Akt participates to endothelial dysfunction in hypertension (2004) Circulation, 109, pp. 2587-2593

Claudio, P.P., Fratta, L., Farina, F., Howard, C.M., Stassi, G., Numata, S., Adenoviral RB2/p130 gene transfer inhibits smooth muscle cell proliferation and prevents restenosis after angioplasty (1999) Circ Res, 85, pp. 1032-1039

Cittadini, A., Stromer, H., Katz, S.E., Clark, R., Moses, A.C., Morgan, J.P., Differential cardiac effects of GH and IGF-1 in the rat: A combined in vivo and in vitro evaluation (1996) Circulation, 93, pp. 800-809

Kihara, Y., Grossman, W., Morgan, J.P., Direct measurement of changes in intracellular calcium transients during hypoxia, ischemia and reperfusion of the intact mammalian heart (1989) Circ. Res., 65, pp. 1029-1044

Brooks, W.W., Healey, N.A., Sen, S., Conrad, C.H., Bing, O.H., Oxygen cost of stress development in hypertrophied and failing hearts from the spontaneously hypertensive rat (1993) Hypertension, 21, pp. 56-64

Nelson, G.S., Berger, R.D., Fetics, B.J., Talbot, M., Spinelli, J.C., Hare, J.M., Left ventricular or biventricular pacing improves cardiac function at diminished energy cost in patients with dilated cardiomyopathy and left bundle-branch block (2000) Circulation, 102, pp. 3053-3059

Callies, F., Stromer, H., Schwinger, R.H., Bolck, B., Hu, K., Frantz, S., Administration of testosterone is associated with a reduced susceptibility to myocardial ischemia (2003) Endocrinology, 144, pp. 4478-4483

Chomczynski, P., Sacchi, N., Single step method of RNA isolation by acidic guanidinium thiocyanate-phenol-chloroform extraction (1987) Anal Biochem, 162, pp. 156-159

Temsah, R.M., Dyck, C., Netticadan, T., Chapman, D., Elimban, V., Dhalla, N.S., Effect of β-adrenoreceptor blockers on sarcoplasmatic reticular function and gene expression in the ischemic reperfused heart (2000) J Pharmacol Exp Ther, 293, pp. 15-23

Iaccarino, G., Barbato, E., Cipolletta, E., Esposito, A., Fiorillo, A., Koch, W.J., Cardiac βARK1 upregulation induced by chronic salt deprivation in rats (2001) Hypertension, 38, pp. 255-260

Nessun risultato.
Nessun risultato.

Contiene: [X]      Estesa  
Autori: [X]
Tipo di lavoro: [X]
Data iniziale: Data finale: [X]
Sede: Affiliazione IBB
    
[Pulisci modulo]
Ciampi Q, Betocchi S, Losi MA, Ferro A, Cuocolo A, Lombardi R, Villari B, Chiariello M
* Abnormal blood-pressure response to exercise and oxygen consumption in patients with hypertrophic cardiomyopathy (71 visite)
J Nucl Cardiol (ISSN: 1532-6551, 1071-3581, 1532-6551electronic), 2007 Nov; 14(6): 869-875.
Dettagli    Esporta in BibTeX    Esporta in EndNote



1 Records (1 escludendo Abstract e Conferenze).
Impact factor totale: 2.359 (2.359 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 2.269 (2.269 escludendo Abstract e Conferenze).







    Esporta in BibTeX    Esporta in EndNote

Ultima modifica di in data Friday 26 June 2015, 14:16:20
112 visite. Ultima visita in data Tuesday 02 October 2018, 6:29:31

Webmaster and developer: Marco Comerci
Per problemi e suggerimenti: adminibb.cnr.it
Ultimo aggiornamento: Tuesday 16 October 2018, 14:35:55