Pegylated Trastuzumab Fragments Acquire an Increased in Vivo Stability but Show a Largely Reduced Affinity for the Target Antigen (177 visite) (PDF pubblico 182 visite)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067linking), 2016 Apr 1; 17(4): N/D-N/D.
Tipo di articolo: Journal Article,
Impact factor: 3.226, Impact factor a 5 anni: 3.482
Url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84962385296&doi=10.3390%2fijms17040491&partnerID=40&md5=35ef6085d9521fc0431927bb55bd407e
Parole chiave: Fab, Pegylation, Antibody Fragment, Papain Digestion, Pepsin Digestion, Pharmacokinetics,
Affiliazioni:
*** IBB - CNR ***
Bioker srl-Multimedica Group, c/o Institute of Genetics and
Biophysics, National Research Council (CNR-IGB) via P. Castellino 111,
Institute of Biostructure and Bioimaging, National Research Council (IBB-CNR), via Mezzocannone 16, 80134 Napoli, Italy
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
(CIRPeB), University of Naples Federico II, via Mezzocannone 16, 80134
Department of Clinical Medicine and Surgery, University of Naples Federico II, 80134 Napoli, Italy
Department of Biomedical, Biotechnological and Translational
Science (S.Bi.Bi.T.), Università di Parma, 43126 Parma, Italy
class="page" title="Page 1">
carlamarra78@yahoo.it (C.M.); silvia.scaramuzza@multimedica.it (S.S.); annalisa.politano@gmail.com (A.P.);
riccardo.sanna@multimedica.it (R.S.); giancarlo.tonon@multimedica.it (G.T.)
80134 Napoli, Italy; giuseppina.foca@gmail.com (G.F.); menotti.ruvo@unina.it (M.R.)
43126 Parma, Italy; gloria.saccani@unipr.it
Correspondence: fabio.selis@multimedica.it (F.S.); annamaria.sandomenico@gmail.com (A.S.);
Tel.: +39-081-613-2423 (F.S.); +39-081-253-6644 (A.S.)
These authors contributed equally to this work.
Riferimenti:
Non disponibile.
Cocozza S, Pontillo G, Lanzillo R, Russo C, Petracca M, Di Stasi M, Paolella C, Vola EA, Criscuolo C, Moccia M, Lamberti A, Monti S, Brescia Morra V, Elefante A, Palma G, Tedeschi E, Brunetti A
* MRI features suggestive of gadolinium retention do not correlate with Expanded Disability Status Scale worsening in Multiple Sclerosis (110 visite)
Neuroradiology (ISSN: 0028-3940linking, 1432-1920electronic), 2019 Feb; 61(2): 155-162.
Impact Factor: 1.287
Dettagli Esporta in BibTeX Esporta in EndNote
Calcagno V, Vecchione R, Quagliariello V, Marzola P, Busato A, Giustetto P, Profeta M, Gargiulo S, Cicco CD, Yu H, Cassani M, Maurea N, Mancini M, Pellegrino T, Netti PA
* Oil Core-PEG Shell Nanocarriers for In Vivo MRI Imaging (33 visite)
Adv Healthc Mater (ISSN: 2192-2640linking), 2019 Feb; 8(3): e1801313-e1801313.
Impact Factor: 5.11
Dettagli Esporta in BibTeX Esporta in EndNote
Gianolio E, Bardini P, Arena F, Stefania R, Di Gregorio E, Iani R, Aime S
* Gadolinium Retention in the Rat Brain: Assessment of the Amounts of Insoluble Gadolinium-containing Species and Intact Gadolinium Complexes after Repeated Administration of Gadolinium-based Contrast Agents (74 visite)
Radiology (ISSN: 0033-8419, 1527-1315), 2017 Dec; 285(3): 839-849.
Impact Factor: 7.469
Dettagli Esporta in BibTeX Esporta in EndNote
Tomasello MF, Nardon C, Lanza V, Di Natale G, Pettenuzzo N, Salmaso S, Milardi D, Caliceti P, Pappalardo G, Fregona D
* New comprehensive studies of a gold(III) Dithiocarbamate complex with proven anticancer properties: Aqueous dissolution with cyclodextrins, pharmacokinetics and upstream inhibition of the ubiquitin-proteasome pathway (148 visite)
Eur J Med Chem (ISSN: 0223-5234, 1768-3254, 0223-5234linking), 2017 Jun 19; 138: 115-127.
Impact Factor: 4.816
Dettagli Esporta in BibTeX Esporta in EndNote
Filippi M, Catanzaro V, Patrucco D, Botta M, Tei L, Terreno E
* First in vivo MRI study on theranostic dendrimersomes (77 visite)
J Control Release (ISSN: 0168-3659), 2017 Feb 28; 248: 45-52.
Impact Factor: 7.877
Dettagli Esporta in BibTeX Esporta in EndNote
Filippi M, Boido M, Pasquino C, Garello F, Boffa C, Terreno E
* Successful in vivo MRI tracking of MSCs labeled with Gadoteridol in a Spinal Cord Injury experimental model (63 visite)
Exp Neurol (ISSN: 0014-4886), 2016 Aug; 282: 66-77.
Impact Factor: 4.706
Dettagli Esporta in BibTeX Esporta in EndNote
Longo DL, Arena F, Consolino L, Minazzi P, Geninatti-crich S, Giovenzana GB, Aime S
* Gd-AAZTA-MADEC, an improved blood pool agent for DCE-MRI studies on mice on 1 T scanners (167 visite)
Biomaterials (ISSN: 0142-9612, 1878-5905electronic), 2015 Oct 16; 75: 47-57.
Impact Factor: 8.387
Dettagli Esporta in BibTeX Esporta in EndNote
Tornatore L, Sandomenico A, Raimondo D, Low C, Rocci A, Tralau-Stewart C, Capece D, D'Andrea D, Bua M, Boyle E, van Duin M, Zoppoli P, Jaxa-Chamiec A, Thotakura AK, Dyson J, Walker BA, Leonardi A, Chambery A, Driessen C, Sonneveld P, Morgan G, Palumbo A, Tramontano A, Rahemtulla A, Ruvo M, Franzoso G
* Cancer-selective targeting of the NF-kappaB survival pathway with GADD45beta/MKK7 inhibitors (331 visite)
Cancer Cell (ISSN: 1535-6108), 2014 Oct 13; 26(4): 495-508.
Impact Factor: 23.523
Dettagli Esporta in BibTeX Esporta in EndNote
Accardo A, Aloj L, Aurilio M, Morelli G, Tesauro D
* Receptor binding peptides for target-selective delivery of nanoparticles encapsulated drugs (280 visite)
Int J Nanomed (ISSN: 1178-2013, 1176-9114, 1176-9114linking), 2014 Mar 27; 9(1): 1537-1557.
Impact Factor: 4.383
Dettagli Esporta in BibTeX Esporta in EndNote
Mancini M, Greco A, Salvatore G, Liuzzi R, Di Maro G, Vergara E, Chiappetta G, Pasquinelli R, Brunetti A, Salvatore M
* Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice (272 visite)
Bmc Med Imaging (ISSN: 1471-2342), 2013 Sep 12; 13(1): 31-31.
Impact Factor: 0.983
Dettagli Esporta in BibTeX Esporta in EndNote
Pascual B, Prieto E, Arbizu J, Marti-climent JM, Penuelas I, Quincoces G, Zarauza R, Pappata S, Masdeu JC
* Decreased carbon-11-flumazenil binding in early Alzheimer's disease (176 visite) (PDF 166 visite)
Brain (ISSN: 0006-8950, 1460-2156electronic, 0006-8950linking), 2012 Sep; 135(Pt9): 2817-2825.
Impact Factor: 9.915
Dettagli Esporta in BibTeX Esporta in EndNote
Digilio G, Menchise V, Gianolio E, Catanzaro V, Carrera C, Napolitano R, Fedeli F, Aime S
* Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling (224 visite)
J Med Chem (ISSN: 0022-2623, 1520-4804, 0022-2623print), 2010 Jul 8; 53(13): 4877-4890.
Impact Factor: 5.207
Dettagli Esporta in BibTeX Esporta in EndNote
Berkouk K, Quarantelli M, Prinster A, Landeau B, Alfano B, Baron JC
* Mapping The Relative Contribution Of Gray Matter Activity Versus Volume In Brain Pet. A New Approach (202 visite) (PDF 153 visite)
J Neuroimaging (ISSN: 1051-2284), 2006 Jul; 16(3): 224-235.
Impact Factor: 1.298
Dettagli Esporta in BibTeX Esporta in EndNote
Aloj L, Panico M, Caraco C, Del Vecchio S, Arra C, Affuso A, Accardo A, Mansi R, Tesauro D, De Luca S, Pedone C, Visentin R, Mazzi U, Morelli G, Salvatore M
* In vitro and in vivo characterization of Indium-111 and Technetium-99m labeled CCK-8 derivatives for CCK-B receptor imaging (216 visite)
Cancer Biotherapy And Radiopharmaceuticals (ISSN: 1084-9785, 1084-4978), 2004 Feb; 19(1): 93-98.
Impact Factor: 1.091
Dettagli Esporta in BibTeX Esporta in EndNote
Andretta C, Catalano L, Pace L, Fonti R, Annunziata G, Rotoli B
* 99mTc-sestaMIBI scintigraphy in thalidomide-treated refractory or relapsed multiple myeloma patients [2] (121 visite)
Leuk Lymphoma (ISSN: 1042-8194, 1026-8022linking), 2003 Jun; 44(6): 1081-1082.
Impact Factor: 1.163
Dettagli Esporta in BibTeX Esporta in EndNote
Maurea S, Lastoria S, Caracò C, Klain M, Varrella P, Acampa W, Muto P, Salvatore M
The role of radiolabeled somatostatin analogs in adrenal imaging (143 visite)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1996 Sep; 23(6): 677-680.
Impact Factor: 1.042
Dettagli Esporta in BibTeX Esporta in EndNote
Trischitta V, Benzi L, Brunetti A, Cecchetti P, Marchetti P, Vigneri R, Navalesi R
Intracellular insulin processing is altered in monocytes from patients with type II diabetes mellitus (111 visite)
J Clin Endocrinol Metab (ISSN: 0021-972x), 1987 May; 64(5): 914-920.
Impact Factor: 4.575
Dettagli Esporta in BibTeX Esporta in EndNote
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067linking), 2016 Apr 1; 17(4): N/D-N/D.
Tipo di articolo: Journal Article,
Impact factor: 3.226, Impact factor a 5 anni: 3.482
Url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84962385296&doi=10.3390%2fijms17040491&partnerID=40&md5=35ef6085d9521fc0431927bb55bd407e
Parole chiave: Fab, Pegylation, Antibody Fragment, Papain Digestion, Pepsin Digestion, Pharmacokinetics,
Affiliazioni:
*** IBB - CNR ***
Bioker srl-Multimedica Group, c/o Institute of Genetics and
Biophysics, National Research Council (CNR-IGB) via P. Castellino 111,
Institute of Biostructure and Bioimaging, National Research Council (IBB-CNR), via Mezzocannone 16, 80134 Napoli, Italy
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
(CIRPeB), University of Naples Federico II, via Mezzocannone 16, 80134
Department of Clinical Medicine and Surgery, University of Naples Federico II, 80134 Napoli, Italy
Department of Biomedical, Biotechnological and Translational
Science (S.Bi.Bi.T.), Università di Parma, 43126 Parma, Italy
class="page" title="Page 1">
carlamarra78@yahoo.it (C.M.); silvia.scaramuzza@multimedica.it (S.S.); annalisa.politano@gmail.com (A.P.);
riccardo.sanna@multimedica.it (R.S.); giancarlo.tonon@multimedica.it (G.T.)
80134 Napoli, Italy; giuseppina.foca@gmail.com (G.F.); menotti.ruvo@unina.it (M.R.)
43126 Parma, Italy; gloria.saccani@unipr.it
Correspondence: fabio.selis@multimedica.it (F.S.); annamaria.sandomenico@gmail.com (A.S.);
Tel.: +39-081-613-2423 (F.S.); +39-081-253-6644 (A.S.)
These authors contributed equally to this work.
Riferimenti:
Non disponibile.
<div class="\\"page\\"" title="\\"Page" 1"=""><div class="\\"layoutArea\\""><div class="\\"column\\""><span style="\\"font-size:" 10.000000pt;="" font-family:="" 'urwpalladiol'"="">PEGylation of biomolecules is a major approach to increase blood stream half-life, stability<br>and solubility of biotherapeutics and to reduce their immunogenicity, aggregation potential and<br>unspecific interactions with other proteins and tissues. Antibodies have generally long half-lives<br>due to high molecular mass and stability toward proteases, however their size lowers to some extent<br>their potential because of a reduced ability to penetrate tissues, especially those of tumor origin.<br>Fab or otherwise engineered smaller fragments are an alternative but are less stable and are much<br>less well retained in circulation. We have here investigated the effects of various PEGylations on<br>the binding properties and </span><span style="\\"font-size:" 10.000000pt;="" font-family:="" 'urwpalladiol';="" font-style:="" italic"="">in vivo </span><span style="\\"font-size:" 10.000000pt;="" font-family:="" 'urwpalladiol'"="">half-life of Fab fragments derived from the enzymatic splitting of<br>Trastuzumab. We find that PEGylation increases the half-life of the molecules but also strongly affects<br>the ability to recognize the target antigen in a way that is dependent on the extent and position of the<br>chemical modification. Data thus support the concept that polyethylene glycol (PEG) conjugation on<br>Trastuzumab Fabs increases half-life but reduces their affinity and this is a fine balance, which must<br>be carefully considered for the design of strategies based on the use of antibody fragments.<br></span><br>
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Vai a fine pagina Versione per copia e incolla facile Esporta tutto in BibTeX Esporta tutto in EndNote
Cocozza S, Pontillo G, Lanzillo R, Russo C, Petracca M, Di Stasi M, Paolella C, Vola EA, Criscuolo C, Moccia M, Lamberti A, Monti S, Brescia Morra V, Elefante A, Palma G, Tedeschi E, Brunetti A
* MRI features suggestive of gadolinium retention do not correlate with Expanded Disability Status Scale worsening in Multiple Sclerosis (110 visite)
Neuroradiology (ISSN: 0028-3940linking, 1432-1920electronic), 2019 Feb; 61(2): 155-162.
Impact Factor: 1.287
Dettagli Esporta in BibTeX Esporta in EndNote
Calcagno V, Vecchione R, Quagliariello V, Marzola P, Busato A, Giustetto P, Profeta M, Gargiulo S, Cicco CD, Yu H, Cassani M, Maurea N, Mancini M, Pellegrino T, Netti PA
* Oil Core-PEG Shell Nanocarriers for In Vivo MRI Imaging (33 visite)
Adv Healthc Mater (ISSN: 2192-2640linking), 2019 Feb; 8(3): e1801313-e1801313.
Impact Factor: 5.11
Dettagli Esporta in BibTeX Esporta in EndNote
Gianolio E, Bardini P, Arena F, Stefania R, Di Gregorio E, Iani R, Aime S
* Gadolinium Retention in the Rat Brain: Assessment of the Amounts of Insoluble Gadolinium-containing Species and Intact Gadolinium Complexes after Repeated Administration of Gadolinium-based Contrast Agents (74 visite)
Radiology (ISSN: 0033-8419, 1527-1315), 2017 Dec; 285(3): 839-849.
Impact Factor: 7.469
Dettagli Esporta in BibTeX Esporta in EndNote
Tomasello MF, Nardon C, Lanza V, Di Natale G, Pettenuzzo N, Salmaso S, Milardi D, Caliceti P, Pappalardo G, Fregona D
* New comprehensive studies of a gold(III) Dithiocarbamate complex with proven anticancer properties: Aqueous dissolution with cyclodextrins, pharmacokinetics and upstream inhibition of the ubiquitin-proteasome pathway (148 visite)
Eur J Med Chem (ISSN: 0223-5234, 1768-3254, 0223-5234linking), 2017 Jun 19; 138: 115-127.
Impact Factor: 4.816
Dettagli Esporta in BibTeX Esporta in EndNote
Filippi M, Catanzaro V, Patrucco D, Botta M, Tei L, Terreno E
* First in vivo MRI study on theranostic dendrimersomes (77 visite)
J Control Release (ISSN: 0168-3659), 2017 Feb 28; 248: 45-52.
Impact Factor: 7.877
Dettagli Esporta in BibTeX Esporta in EndNote
Filippi M, Boido M, Pasquino C, Garello F, Boffa C, Terreno E
* Successful in vivo MRI tracking of MSCs labeled with Gadoteridol in a Spinal Cord Injury experimental model (63 visite)
Exp Neurol (ISSN: 0014-4886), 2016 Aug; 282: 66-77.
Impact Factor: 4.706
Dettagli Esporta in BibTeX Esporta in EndNote
Longo DL, Arena F, Consolino L, Minazzi P, Geninatti-crich S, Giovenzana GB, Aime S
* Gd-AAZTA-MADEC, an improved blood pool agent for DCE-MRI studies on mice on 1 T scanners (167 visite)
Biomaterials (ISSN: 0142-9612, 1878-5905electronic), 2015 Oct 16; 75: 47-57.
Impact Factor: 8.387
Dettagli Esporta in BibTeX Esporta in EndNote
Tornatore L, Sandomenico A, Raimondo D, Low C, Rocci A, Tralau-Stewart C, Capece D, D'Andrea D, Bua M, Boyle E, van Duin M, Zoppoli P, Jaxa-Chamiec A, Thotakura AK, Dyson J, Walker BA, Leonardi A, Chambery A, Driessen C, Sonneveld P, Morgan G, Palumbo A, Tramontano A, Rahemtulla A, Ruvo M, Franzoso G
* Cancer-selective targeting of the NF-kappaB survival pathway with GADD45beta/MKK7 inhibitors (331 visite)
Cancer Cell (ISSN: 1535-6108), 2014 Oct 13; 26(4): 495-508.
Impact Factor: 23.523
Dettagli Esporta in BibTeX Esporta in EndNote
Accardo A, Aloj L, Aurilio M, Morelli G, Tesauro D
* Receptor binding peptides for target-selective delivery of nanoparticles encapsulated drugs (280 visite)
Int J Nanomed (ISSN: 1178-2013, 1176-9114, 1176-9114linking), 2014 Mar 27; 9(1): 1537-1557.
Impact Factor: 4.383
Dettagli Esporta in BibTeX Esporta in EndNote
Mancini M, Greco A, Salvatore G, Liuzzi R, Di Maro G, Vergara E, Chiappetta G, Pasquinelli R, Brunetti A, Salvatore M
* Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice (272 visite)
Bmc Med Imaging (ISSN: 1471-2342), 2013 Sep 12; 13(1): 31-31.
Impact Factor: 0.983
Dettagli Esporta in BibTeX Esporta in EndNote
Pascual B, Prieto E, Arbizu J, Marti-climent JM, Penuelas I, Quincoces G, Zarauza R, Pappata S, Masdeu JC
* Decreased carbon-11-flumazenil binding in early Alzheimer's disease (176 visite) (PDF 166 visite)
Brain (ISSN: 0006-8950, 1460-2156electronic, 0006-8950linking), 2012 Sep; 135(Pt9): 2817-2825.
Impact Factor: 9.915
Dettagli Esporta in BibTeX Esporta in EndNote
Digilio G, Menchise V, Gianolio E, Catanzaro V, Carrera C, Napolitano R, Fedeli F, Aime S
* Exofacial protein thiols as a route for the internalization of Gd(III)-based complexes for magnetic resonance imaging cell labeling (224 visite)
J Med Chem (ISSN: 0022-2623, 1520-4804, 0022-2623print), 2010 Jul 8; 53(13): 4877-4890.
Impact Factor: 5.207
Dettagli Esporta in BibTeX Esporta in EndNote
Berkouk K, Quarantelli M, Prinster A, Landeau B, Alfano B, Baron JC
* Mapping The Relative Contribution Of Gray Matter Activity Versus Volume In Brain Pet. A New Approach (202 visite) (PDF 153 visite)
J Neuroimaging (ISSN: 1051-2284), 2006 Jul; 16(3): 224-235.
Impact Factor: 1.298
Dettagli Esporta in BibTeX Esporta in EndNote
Aloj L, Panico M, Caraco C, Del Vecchio S, Arra C, Affuso A, Accardo A, Mansi R, Tesauro D, De Luca S, Pedone C, Visentin R, Mazzi U, Morelli G, Salvatore M
* In vitro and in vivo characterization of Indium-111 and Technetium-99m labeled CCK-8 derivatives for CCK-B receptor imaging (216 visite)
Cancer Biotherapy And Radiopharmaceuticals (ISSN: 1084-9785, 1084-4978), 2004 Feb; 19(1): 93-98.
Impact Factor: 1.091
Dettagli Esporta in BibTeX Esporta in EndNote
Andretta C, Catalano L, Pace L, Fonti R, Annunziata G, Rotoli B
* 99mTc-sestaMIBI scintigraphy in thalidomide-treated refractory or relapsed multiple myeloma patients [2] (121 visite)
Leuk Lymphoma (ISSN: 1042-8194, 1026-8022linking), 2003 Jun; 44(6): 1081-1082.
Impact Factor: 1.163
Dettagli Esporta in BibTeX Esporta in EndNote
Maurea S, Lastoria S, Caracò C, Klain M, Varrella P, Acampa W, Muto P, Salvatore M
The role of radiolabeled somatostatin analogs in adrenal imaging (143 visite)
Nucl Med Biol (ISSN: 0969-8051, 0883-2897), 1996 Sep; 23(6): 677-680.
Impact Factor: 1.042
Dettagli Esporta in BibTeX Esporta in EndNote
Trischitta V, Benzi L, Brunetti A, Cecchetti P, Marchetti P, Vigneri R, Navalesi R
Intracellular insulin processing is altered in monocytes from patients with type II diabetes mellitus (111 visite)
J Clin Endocrinol Metab (ISSN: 0021-972x), 1987 May; 64(5): 914-920.
Impact Factor: 4.575
Dettagli Esporta in BibTeX Esporta in EndNote
17 Records (17 escludendo Abstract e Conferenze).
Impact factor totale: 92.832 (92.832 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 105.801 (105.801 escludendo Abstract e Conferenze).
Impact factor totale: 92.832 (92.832 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 105.801 (105.801 escludendo Abstract e Conferenze).
Esporta in BibTeX Esporta in EndNote
Ultima modifica di Marco Comerci in data Thursday 21 April 2016, 14:59:09
177 visite. Ultima visita in data Saturday 09 November 2019, 18:41:14
ibb.cnr.it