Export to BibTeX Export to EndNoteCationic porphyrins are tunable gatekeepers of the 20S proteasome (418 views) (PDF restricted 88 views)
Santoro AM, Cunsolo A, D'Urso A, Sbardella D, Tundo GR, Ciaccio C, Coletta M, Diana D, Fattorusso R, Persico M, Di Dato A, Fattorusso C, Milardi D, Purrello R
Chemical Science (ISSN: 2041-6539, 2041-6520), 2016; 7(2): 1286-1297.
Abstract
The 20S proteasome is a barrel-shaped enzymatic assembly playing a critical role in proteome maintenance. Access of proteasome substrates to the catalytic chamber is finely regulated through gating mechanisms which involve aromatic and negatively charged residues located at the N-terminal tails of α subunits. However, despite the importance of gates in regulating proteasome function, up to now very few molecules have been shown to interfere with the equilibrium by which the catalytic channel exchanges between the open and closed states. In this light, and inspired by previous results evidencing the antiproteasome potential of cationic porphyrins, here we combine experimental (enzyme kinetics, UV stopped flow and NMR) and computational (bioinformatic analysis and docking studies) approaches to inspect proteasome inhibition by meso-tetrakis(4-N-methylpyridyl)-porphyrin (H2T4) and its two ortho- and meta-isomers. We show that in a first, fast binding event H2T4 accommodates in a pocket made of negatively charged and aromatic residues present in α1 (Asp10, Phe9), α3 (Tyr5), α5 (Asp9, Tyr8), α6 (Asp7, Tyr6) and α7 (Asp9, Tyr8) subunits thereby stabilizing the closed conformation. A second, slower binding mode involves interaction with the grooves which separate the α- from the β-rings. Of note, the proteasome inhibition by ortho- and meta-H2T4 decreases significantly if compared to the parent compound, thus underscoring the role played by spatial distribution of the four peripheral positive charges in regulating proteasome–ligand interactions. We think that our results may pave the way to further studies aimed at rationalizing the molecular basis of novel, and more sophisticated, proteasome regulatory mechanisms.
Affiliations ▼
*** IBB - CNR Affiliation
Istituto di Biostrutture e Bioimmagini – CNR UOS di Catania, Via P. Gaifami 18, 95126 Catania, Italy.
Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, 95125 Catania, Italy.
Dipartimento di Farmacia Università di Napoli “Federico II”, Via D. Montesano, 49 I-80131 Napoli, Italy. |
Details ▼
Impact factor: 9.211, 5-year impact factor: 0
Paper type: Journal Article,
Keywords: Aromatic Compounds, Binding Energy, Bioinformatics, Enzyme Kinetics, Isomers, Porphyrins, Reconfigurable Hardware, Aromatic Residues, Bioinformatic Analysis, Catalytic Chambers, Cationic Porphyrin, Gating Mechanisms, Ligand Interactions, Negatively Charged, Regulatory Mechanism, Enzyme Inhibition,
Url: http://dx.doi.org/10.1039/C5SC03312H |
References ▼
D. Finley, Annu. Rev. Biochem., 2009, 78, 477 CrossRef CAS PubMed.S. Murata, H. Yashiroda and K. Tanaka, Nat. Rev. Mol. Cell Biol., 2009, 10, 104 CrossRef CAS PubMed.N. Gallastegui and M. Groll, Trends Biochem. Sci., 2010, 35, 634 CrossRef CAS PubMed.O. Coux, K. Tanaka and A. L. Goldberg, Annu. Rev. Biochem., 1996, 65, 801 CrossRef CAS PubMed.W. Baumeister, J. Walz, F. Zühl and E. Seemüller, Cell, 1998, 92, 367 CrossRef CAS PubMed.M. H. Glickman, D. M. Rubin, O. Coux, I. Wefes, G. Pfeifer, Z. Cjeka, W. Baumeister, V. A. Fried and D. Finley, Cell, 1998, 94(5), 6156 CrossRef.C. S. Arendt and M. Hochstrasser, Proc. Natl. Acad. Sci. U. S. A., 1997, 94, 7156 CrossRef CAS.W. Heinemeyer, M. Fischer, T. Krimmer, U. Stachon and D. H. Wolf, J. Biol. Chem., 1997, 272, 25200 CrossRef CAS PubMed.A. F. Kisselev, A. Callard and A. L. Goldberg, J. Biol. Chem., 2006, 281, 8582 CrossRef CAS PubMed.M. Groll, L. Ditzel, J. Löwe, D. Stock, M. Bochtler, H. D. Bartunik and R. Huber, Nature, 1997, 386, 463 CrossRef CAS PubMed.M. Groll, M. Bajorek, A. Kohler, L. Moroder, D. M. Rubin, R. Huber, M. Glickman and D. Finley, Nat. Struct. Biol., 2000, 7, 1062 CrossRef CAS PubMed.M. Groll and R. Huber, Int. J. Biochem. Cell Biol., 2003, 5, 606 CrossRef.A. Kohler, M. Bajorek, M. Groll, L. Moroder, D. M. Rubin, R. Huber, M. Glickman and D. Finley, Biochimie, 2001, 83, 325 CrossRef CAS PubMed.D. J. McConkey and K. Zhu, Drug Resist. Updates, 2008, 11, 164 CrossRef CAS PubMed.A. Lehmann, K. Jechow and C. Enenkel, EMBO Rep., 2008, 9(12), 1237 CrossRef CAS PubMed.G. Ben-Nissan and M. Sharon, Biomolecules, 2014, 4, 862 CrossRef CAS PubMed.E. A. Obeng, L. M. Carlson, D. M. Gutman, W. J. Harrington Jr, K. P. Lee and L. H. Boise, Blood, 2006, 107(12), 4907 CrossRef CAS PubMed.S. T. Nawrocki, J. S. Carew, K. Dunner Jr, L. H. Boise, P. J. Chiao, P. Huang, J. L. Abbruzzese and D. J. McConkey, Cancer Res., 2005, 65(24), 11510 CrossRef CAS PubMed.G. Bianchi, L. Oliva, P. Cascio, N. Pengo, F. Fontana, F. Cerruti, A. Orsi, E. Pasqualetto, A. Mezghrani, V. Calbi, G. Palladini, N. Giuliani, K. C. Anderson, R. Sitia and S. Cenci, Blood, 2009, 13(13), 3040 CrossRef PubMed.L. R. Dick and P. E. Fleming, Drug Discovery Today, 2010, 15(5–6), 243 CrossRef CAS PubMed.E. M. Huber and M. Groll, Angew. Chem., Int. Ed. Engl., 2012, 51(35), 8708 CrossRef CAS PubMed.M. Bazzaro, M. K. Lee, A. Zoso, W. L. H. Stirling, A. Santillan, I.-M. Shih and R. B. S. Roden, Cancer Res., 2006, 66(7), 3754 CrossRef CAS PubMed.O. A. O'Connor, J. Wright, C. Moskowitz, J. Muzzy, B. MacGregor-Cortelli, M. Stubblefield, D. Straus, C. Portlock, P. Hamlin, E. Choi, O. Dumetrescu, D. Esseltine, E. Trehu, J. Adams, D. Schenkein and A. D. Zelenetz, J. Clin. Oncol., 2005, 23(4), 676 CrossRef PubMed.J. Wolf, P. G. Richardson, M. Schuster, A. LeBlanc, I. B. Walters and D. S. Battleman, Clin. Adv. Hematol. Oncol., 2008, 6(10), 755–760 Search PubMed.D. J. Kuhn, R. Z. Orlowski and C. C. Bjorklund, Curr. Cancer Drug Targets, 2011, 11(3), 285 CrossRef CAS PubMed.S. Kumar and S. V. Rajkumar, Blood, 2008, 112(6), 2177 CrossRef CAS PubMed.E. M. Huber, W. Heinemeyer and M. Groll, Structure, 2015, 23, 407–417 CrossRef CAS PubMed.X. Li, T. E. Wood, R. Sprangers, G. Jansen, N. E. Franke, X. Mao, X. Wang, Y. Zhang, S. E. Verbrugge, H. Adomat, Z. H. Li, S. Trudel, C. Chen, T. L. Religa, N. Jamal, H. Messner, J. Cloos, D. R. Rose, A. Navon, E. Guns, R. A. Batey, L. E. Kay and A. D. Schimmer, J. Natl. Cancer Inst., 2010, 102(14), 1069 CrossRef CAS PubMed.J. Li, M. Post, R. Volk, Y. Gao, M. Li, C. Metais, K. Sato, J. Tsai, W. Aird, R. D. Rosenberg, T. G. Hampton, F. Sellke, P. Carmeliet and M. Simons, Nat. Med., 2000, 6(1), 49 CrossRef CAS PubMed.A. M. Santoro, M. C. Lo Giudice, A. D'Urso, R. Lauceri, R. Purrello and D. Milardi, J. Am. Chem. Soc., 2012, 134, 10451 CrossRef CAS PubMed.F. Vallelian, J. W. Deuel, L. Opitz, C. A. Schaer, M. Puglia, M. Lönn, W. Engelsberger, S. Schauer, E. Karnaukhova, D. R. Spahn, R. Stocker, P. W. Buehler and D. J. Schaer, Cell Death Differ., 2015, 22(4), 597 CrossRef CAS PubMed.T. J. Dougherty, C. J. Gomer, B. W. Henderson, G. Jori, D. Kessel, M. Korbelik, J. Moan and Q. Peng, J. Natl. Cancer Inst., 1998, 90(12), 889 CrossRef CAS PubMed.V. Rapozzi, S. Zorzet, M. Zacchigna, E. Della Pietra, S. Cogoi and L. E. Xodo, Mol. Cancer, 2014, 13(75), 1 Search PubMed.J. Klages, M. Coles and H. Kessler, NMR-Based Screening: A Powerful Tool in Fragment-Based Drug Discovery, in Exploiting Chemical Diversity for Drug Discovery, ed. P. A. Bartlett and M. Entzeroth, RSC Publishing, Cambridge, 2006, ch. 12, pp. 263–290 and Mol. BioSyst., 2006, vol. 2, pp. 318–332 Search PubMed.B. Meyer and T. Peters, Angew. Chem., Int. Ed. Engl., 2003, 42(8), 864 CrossRef CAS PubMed.J. Klein, R. Meinecke, M. Mayer and B. Meyer, J. Am. Chem. Soc., 1999, 121(22), 5336 CrossRef CAS.ACD/Percepta, version 14.0.0, Advanced Chemistry Development, Inc., Toronto, ON, Canada, 2013, http://www.acdlabs.com Search PubMed.P. Dauber-Osguthorpe, V. A. Roberts, D. J. Osguthorpe, J. Wolff, M. Genest and A. T. Hagler, Proteins, 1998, 4, 31 CrossRef PubMed.M. J. S. Dewar and W. Thiel, J. Am. Chem. Soc., 1977, 99, 4899 CrossRef CAS.I. W. Davis, A. Leaver-Fay, V. B. Chen, J. N. Block, G. J. Kapral, X. Wang, L. W. Murray, W. B. Arendall, J. Snoeyink, J. S. Richardson and D. C. Richardson, Nucleic Acids Res., 2007, 35, W375 CrossRef PubMed.M. Mayer and B. Meyer, J. Am. Chem. Soc., 2001, 123, 6108 CrossRef CAS PubMed.J. Pons, N. Evrad-Todeschi, G. Bertho, J. Gharbi Benarous, V. Tanchou, R. Benarous and J. P. Girault, Biochemistry, 2008, 47, 14 CrossRef CAS PubMed.L. Cigliano, L. de Rosa, D. Diana, R. Di Stasi, M. S. Spagnuolo, B. Maresca, R. Fattorusso and L. D. D'Andrea, RSC Adv., 2014, 4, 51353 RSC.W. Harshbarger, C. Miller, C. Diedrich and J. Sacchettini, Structure, 2015, 23(2), 418 CrossRef CAS PubMed.J. Pei, B. H. Kim and N. V. Grishin, Nucleic Acids Res., 2008, 36, 2295 CrossRef CAS PubMed.J. Rabl, D. M. Smith, Y. Yu, S. C. Chang, A. L. Goldberg and Y. Cheng, Mol. Cell, 2008, 30(3), 360 CrossRef CAS PubMed.A. Förster, E. I. Masters, F. G. Whitby, H. Robinson and C. P. Hill, Mol. Cell, 2005, 18(5), 589 CrossRef PubMed.A. M. Ruschak and L. E. Kay, Proc. Natl. Acad. Sci. U. S. A., 2012, 109(50), E3454 CrossRef CAS PubMed.F. G. Whitby, E. I. Masters, L. Kramer, J. R. Knowlton, Y. Yao, C. C. Wang and C. P. Hill, Nature, 2000, 408, 115 CrossRef CAS PubMed.E. Jankowska, M. Gaczynska, P. A. Osmulski, E. Sikorska, R. Rostankowski, S. Madabhushi, M. Tokmina-Lukaszewska and F. Kasprzykowski, Biopolymers, 2010, 93, 481 CrossRef CAS PubMed.J. Witkowska, P. Karpowicz, M. Gaczynska, P. A. Osmulski and E. Jankowska, J. Pept. Sci., 2014, 20, 649 CrossRef CAS PubMed.M. Groll, H. Brandstetter, H. D. Bartunik, G. Bourenkow and R. Huber, J. Mol. Biol., 2003, 327, 75 CrossRef CAS PubMed.M. Orlowski, C. Cardozo, M. C. Hidalgo and C. Michaud, Biochemistry, 1991, 30, 5999 CrossRef CAS PubMed.P. A. Osmulski, M. Hochstrasser and M. Gaczynska, Structure, 2009, 17(8), 1137 CrossRef CAS PubMed.G. G. Hammes, Y. C. Chang and T. G. Oas, Proc. Natl. Acad. Sci. U. S. A., 2009, 106(33), 13737 CrossRef CAS PubMed.R. Sprangers, X. Li, X. Mao, J. L. Rubinstein, A. D. Schimmer and L. E. Kay, Biochemistry, 2008, 47(26), 6727 CrossRef CAS PubMed.M. Gaczynska and P. A. Osmulski, Methods Enzymol., 2005, 398, 425 CAS.A. F. Kisselev, T. N. Akopian and A. L. Goldberg, J. Biol. Chem., 1998, 273, 1982 CrossRef CAS PubMed.P. Tsvetkov, N. Reuven, C. Prives and Y. Shaul, J. Biol. Chem., 2009, 284, 26234 CrossRef CAS PubMed.M. Bajorek, D. Finley and M. H. Glickman, Curr. Biol., 2003, 13(13), 1140 CrossRef CAS PubMed.
Finley, D., (2009) Annu. Rev. Biochem., 78, p. 47
Murata, S., Yashiroda, H., Tanaka, K., (2009) Nat. Rev. Mol. Cell Biol., 10, p. 104
Gallastegui, N., Groll, M., (2010) Trends Biochem. Sci., 35, p. 634
Coux, O., Tanaka, K., Goldberg, A.L., (1996) Annu. Rev. Biochem., 65, p. 801
Baumeister, W., Walz, J., Zühl, F., Seemüller, E., (1998) Cell, 92, p. 367
Glickman, M.H., Rubin, D.M., Coux, O., Wefes, I., Pfeifer, G., Cjeka, Z., Baumeister, W., Finley, D., (1998) Cell, 94 (5), p. 6156
Arendt, C.S., Hochstrasser, M., (1997) Proc. Natl. Acad. Sci. U. S. A., 94, p. 7156
Heinemeyer, W., Fischer, M., Krimmer, T., Stachon, U., Wolf, D.H., (1997) J. Biol. Chem., 272, p. 25200
Kisselev, A.F., Callard, A., Goldberg, A.L., (2006) J. Biol. Chem., 281, p. 8582
Groll, M., Ditzel, L., Löwe, J., Stock, D., Bochtler, M., Bartunik, H.D., Huber, R., (1997) Nature, 386, p. 463
Groll, M., Bajorek, M., Kohler, A., Moroder, L., Rubin, D.M., Huber, R., Glickman, M., Finley, D., (2000) Nat. Struct. Biol., 7, p. 1062
Groll, M., Huber, R., (2003) Int. J. Biochem. Cell Biol., 5, p. 606
Kohler, A., Bajorek, M., Groll, M., Moroder, L., Rubin, D.M., Huber, R., Glickman, M., Finley, D., (2001) Biochimie, 83, p. 325
McConkey, D.J., Zhu, K., (2008) Drug Resist. Updates, 11, p. 164
Lehmann, A., Jechow, K., Enenkel, C., (2008) EMBO Rep., 9 (12), p. 1237
Ben-Nissan, G., Sharon, M., (2014) Biomolecules, 4, p. 862
Obeng, E.A., Carlson, L.M., Gutman, D.M., Harrington, W.J., Jr., Lee, K.P., Boise, L.H., (2006) Blood, 107 (12), p. 4907
Nawrocki, S.T., Carew, J.S., Dunner, K., Jr., Boise, L.H., Chiao, P.J., Huang, P., Abbruzzese, J.L., McConkey, D.J., (2005) Cancer Res., 65 (24), p. 11510
Bianchi, G., Oliva, L., Cascio, P., Pengo, N., Fontana, F., Cerruti, F., Orsi, A., Cenci, S., (2009) Blood, 13 (13), p. 3040
Dick, L.R., Fleming, P.E., (2010) Drug Discovery Today, 15 (56), p. 243
Huber, E.M., Groll, M., (2012) Angew. Chem., Int. Ed. Engl., 51 (35), p. 8708
Bazzaro, M., Lee, M.K., Zoso, A., Stirling, W.L.H., Santillan, A., Shih, I.-M., Roden, R.B.S., (2006) Cancer Res., 66 (7), p. 3754
O'Connor, O.A., Wright, J., Moskowitz, C., Muzzy, J., MacGregor-Cortelli, B., Stubblefield, M., Straus, D., Zelenetz, A.D., (2005) J. Clin. Oncol., 23 (4), p. 676
Wolf, J., Richardson, P.G., Schuster, M., LeBlanc, A., Walters, I.B., Battleman, D.S., (2008) Clin. Adv. Hematol. Oncol., 6 (10), pp. 755-760
Kuhn, D.J., Orlowski, R.Z., Bjorklund, C.C., (2011) Curr. Cancer Drug Targets, 11 (3), p. 285
Kumar, S., Rajkumar, S.V., (2008) Blood, 112 (6), p. 2177
Huber, E.M., Heinemeyer, W., Groll, M., (2015) Structure, 23, pp. 407-417
Li, X., Wood, T.E., Sprangers, R., Jansen, G., Franke, N.E., Mao, X., Wang, X., Schimmer, A.D., (2010) J. Natl. Cancer Inst., 102 (14), p. 1069
Li, J., Post, M., Volk, R., Gao, Y., Li, M., Metais, C., Sato, K., Simons, M., (2000) Nat. Med., 6 (1), p. 49
Santoro, A.M., Lo Giudice, M.C., D'Urso, A., Lauceri, R., Purrello, R., Milardi, D., (2012) J. Am. Chem. Soc., 134, p. 10451
Vallelian, F., Deuel, J.W., Opitz, L., Schaer, C.A., Puglia, M., Lönn, M., Engelsberger, W., Schaer, D.J., (2015) Cell Death Differ., 22 (4), p. 597
Dougherty, T.J., Gomer, C.J., Henderson, B.W., Jori, G., Kessel, D., Korbelik, M., Moan, J., Peng, Q., (1998) J. Natl. Cancer Inst., 90 (12), p. 889
Rapozzi, V., Zorzet, S., Zacchigna, M., Della Pietra, E., Cogoi, S., Xodo, L.E., (2014) Mol. Cancer, 13 (75), p. 1
Klages, J., Coles, M., Kessler, H., NMR-Based Screening: A Powerful Tool in Fragment-Based Drug Discovery (2006) Exploiting Chemical Diversity for Drug Discovery, pp. 263-290. , ed. A. Bartlett and M. Entzeroth, RSC Publishing, Cambridge, ch. 12, and Mol. BioSyst., 2006, 2, 318-332
Meyer, B., Peters, T., (2003) Angew. Chem., Int. Ed. Engl., 42 (8), p. 864
Klein, J., Meinecke, R., Mayer, M., Meyer, B., (1999) J. Am. Chem. Soc., 121 (22), p. 5336
(2013) ACD/Percepta, Version 14.0.0, , http://www.acdlabs.com, Advanced Chemistry Development, Inc., Toronto, ON, Canada
Dauber-Osguthorpe, P., Roberts, V.A., Osguthorpe, D.J., Wolff, J., Genest, M., Hagler, A.T., (1998) Proteins, 4, p. 31
Dewar, M.J.S., Thiel, W., (1977) J. Am. Chem. Soc., 99, p. 4899
Davis, I.W., Leaver-Fay, A., Chen, V.B., Block, J.N., Kapral, G.J., Wang, X., Murray, L.W., Richardson, D.C., (2007) Nucleic Acids Res., 35, p. W375
Pons, J., Evrad-Todeschi, N., Bertho, G., Gharbi Benarous, J., Tanchou, V., Benarous, R., Girault, J.P., (2008) Biochemistry, 47, p. 14
Cigliano, L., De Rosa, L., Diana, D., Di Stasi, R., Spagnuolo, M.S., Maresca, B., Fattorusso, R., D'Andrea, L.D., (2014) RSC Adv., 4, p. 51353
Harshbarger, W., Miller, C., Diedrich, C., Sacchettini, J., (2015) Structure, 23 (2), p. 418
Pei, J., Kim, B.H., Grishin, N.V., (2008) Nucleic Acids Res., 36, p. 2295
Rabl, J., Smith, D.M., Yu, Y., Chang, S.C., Goldberg, A.L., Cheng, Y., (2008) Mol. Cell, 30 (3), p. 360
Förster, A., Masters, E.I., Whitby, F.G., Robinson, H., Hill, C.P., (2005) Mol. Cell, 18 (5), p. 589
Ruschak, A.M., Kay, L.E., (2012) Proc. Natl. Acad. Sci. U. S. A., 109 (50), p. E3454
Whitby, F.G., Masters, E.I., Kramer, L., Knowlton, J.R., Yao, Y., Wang, C.C., Hill, C.P., (2000) Nature, 408, p. 115
Jankowska, E., Gaczynska, M., Osmulski, P.A., Sikorska, E., Rostankowski, R., Madabhushi, S., Tokmina-Lukaszewska, M., Kasprzykowski, F., (2010) Biopolymers, 93, p. 481
Witkowska, J., Karpowicz, P., Gaczynska, M., Osmulski, P.A., Jankowska, E., (2014) J. Pept. Sci., 20, p. 649
Groll, M., Brandstetter, H., Bartunik, H.D., Bourenkow, G., Huber, R., (2003) J. Mol. Biol., 327, p. 75
Orlowski, M., Cardozo, C., Hidalgo, M.C., Michaud, C., (1991) Biochemistry, 30, p. 5999
Osmulski, P.A., Hochstrasser, M., Gaczynska, M., (2009) Structure, 17 (8), p. 1137
Hammes, G.G., Chang, Y.C., Oas, T.G., (2009) Proc. Natl. Acad. Sci. U. S. A., 106 (33), p. 13737
Sprangers, R., Li, X., Mao, X., Rubinstein, J.L., Schimmer, A.D., Kay, L.E., (2008) Biochemistry, 47 (26), p. 6727
Kisselev, A.F., Akopian, T.N., Goldberg, A.L., (1998) J. Biol. Chem., 273, p. 1982
Tsvetkov, P., Reuven, N., Prives, C., Shaul, Y., (2009) J. Biol. Chem., 284, p. 26234
Bajorek, M., Finley, D., Glickman, M.H., (2003) Curr. Biol., 13 (13), p. 1140
|
Related activities ▼
Related papers ▼
Related bibliography ▼
<=2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 ALL YEARS
Sort by: date ▼▲ title ▲ ▼ journal ▲ ▼ authors ▲ ▼ impact factor ▲ ▼* Lead Optimization of P5U and Urantide: Discovery of Novel Potent Ligands at the Urotensin-II Receptor (100 views)Carotenuto A, Auriemma L, Merlino F, Yousif AM, Marasco D, Limatola A, Campiglia P, Gomez-Monterrey I, Santicioli P, Meini S, Maggi CA, Novellino E, Grieco P
J Med Chem (ISSN: 0022-2623, 1520-4804, 0022-2623print), 2014 Jul 24; 57(14): 5965-5974.
View Export to BibTeX Export to EndNote Upload PDF
* Probing The Peptidylglycine Alpha-Hydroxylating Monooxygenase Active Site With Novel 4-Phenyl-3-Butenoic Acid Based Inhibitors (103 views)Langella E, Pierre S, Ghattas W, Giorgi M, Réglier M, Saviano M, Esposito L, Hardré R
Chemmedchem (ISSN: 1860-7179, 1860-7187, 1860-7187electronic), 2010 Sep 3; 5(9): 1568-1576.
View Export to BibTeX Export to EndNote Upload PDF
* Carbonic anhydrase inhibitors. Comparison of aliphatic sulfamate/bis- sulfamate adducts with isozymes II and IX as a platform for designing tight-binding, more isoform-selective inhibitors (92 views)Vitale RM, Alterio V, Innocenti A, Winum JY, Monti SM, De Simone G, Supuran CT
J Med Chem (ISSN: 0022-2623, 1520-4804, 0022-2623print), 2009 Oct 8; 52(19): 5990-5998.
View Export to BibTeX Export to EndNote Upload PDF
* Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (108 views)Di Fiore A, Fiorentino G, Vitale RM, Ronca R, Amodeo P, Pedone C, Bartolucci S, De Simone G
J Mol Biol Journal Of Molecular Biology (ISSN: 1089-8638, 0022-2836, 1089-8638electronic), 2009 May 8; 388(3): 559-569.
View Export to BibTeX Export to EndNote Upload PDF
8 Records ( 8 excluding Abstracts and Conferences). Total impact factor: 29.499 ( 29.499 excluding Abstracts and Conferences). Total 5-year impact factor: 29.763 ( 29.763 excluding Abstracts and Conferences).
Your bibliography query: (([btitle, keywords, abstract] AROMATIC AND [btitle, keywords, abstract] COMPOUNDS AND [btitle, keywords, abstract] BINDING)) AND NOT [id] = 53183
|
|
Last modified by Marco Comerci on Wednesday 20 April 2016, 12:08:17
418 views. Last viewed on Monday 09 October 2017, 9:48:20
|
INCIPIT
ISTAPCA (857 views)
Euro-BioImaging
Instruct-IT
Fondazione Veronesi
Fondo Europeo Pesca (271 views)
INMiND (933 views)
PRIN (1033 views)
eHealthNet (856 views)
Ponrec (812 views)
MFAG Grant (879 views)
