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Cationic porphyrins are tunable gatekeepers of the 20S proteasome (441 visite) (PDF privato 108 visite)

Santoro AM, Cunsolo A, D'Urso A, Sbardella D, Tundo GR, Ciaccio C, Coletta M, Diana D, Fattorusso R, Persico M, Di Dato A, Fattorusso C, Milardi D, Purrello R

Chemical Science (ISSN: 2041-6539, 2041-6520), 2016; 7(2): 1286-1297.

Tipo di articolo: Journal Article,

Impact factor: 8.668

Impact factor a 5 anni: 8.733

Parole chiave: Aromatic Compounds, Binding Energy, Bioinformatics, Enzyme Kinetics, Isomers, Porphyrins, Reconfigurable Hardware, Aromatic Residues, Bioinformatic Analysis, Catalytic Chambers, Cationic Porphyrin, Gating Mechanisms, Ligand Interactions, Negatively Charged, Regulatory Mechanism, Enzyme Inhibition,

Url: http://dx.doi.org/10.1039/C5SC03312H

The 20S proteasome is a barrel-shaped enzymatic assembly playing a critical role in proteome maintenance. Access of proteasome substrates to the catalytic chamber is finely regulated through gating mechanisms which involve aromatic and negatively charged residues located at the N-terminal tails of α subunits. However, despite the importance of gates in regulating proteasome function, up to now very few molecules have been shown to interfere with the equilibrium by which the catalytic channel exchanges between the open and closed states. In this light, and inspired by previous results evidencing the antiproteasome potential of cationic porphyrins, here we combine experimental (enzyme kinetics, UV stopped flow and NMR) and computational (bioinformatic analysis and docking studies) approaches to inspect proteasome inhibition by meso-tetrakis(4-N-methylpyridyl)-porphyrin (H2T4) and its two ortho- and meta-isomers. We show that in a first, fast binding event H2T4 accommodates in a pocket made of negatively charged and aromatic residues present in α1 (Asp10, Phe9), α3 (Tyr5), α5 (Asp9, Tyr8), α6 (Asp7, Tyr6) and α7 (Asp9, Tyr8) subunits thereby stabilizing the closed conformation. A second, slower binding mode involves interaction with the grooves which separate the α- from the β-rings. Of note, the proteasome inhibition by ortho- and meta-H2T4 decreases significantly if compared to the parent compound, thus underscoring the role played by spatial distribution of the four peripheral positive charges in regulating proteasome–ligand interactions. We think that our results may pave the way to further studies aimed at rationalizing the molecular basis of novel, and more sophisticated, proteasome regulatory mechanisms.
*** IBB - CNR ***

Istituto di Biostrutture e Bioimmagini – CNR UOS di Catania, Via P. Gaifami 18, 95126 Catania, Italy.

Dipartimento di Scienze Chimiche, Università di Catania, Viale Andrea Doria 6, 95125 Catania, Italy.

Dipartimento di Farmacia Università di Napoli “Federico II”, Via D. Montesano, 49 I-80131 Napoli, Italy.
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    Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2006; 65(2): 524-524.
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    * From achiral porphyrins to template-imprinted chiral aggregates and further. Self-replication of chiral memory from scratch (70 visite)
    Lauceri R, Raudino A, Scolaro LM, Micali N, Purrello R
    J Am Chem Soc (ISSN: 0002-7863print, 0002-7863linking), 2002 Feb 13; 120(47): 894-895.
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    * Interactions of anionic carboranylated porphyrins with DNA [3] (64 visite)
    Lauceri R, Purrello R, Shetty SJ, Vicente MGH
    J Am Chem Soc (ISSN: 0002-7863, 0002-2786, 1520-5126), 2001 Jun 20; 123(24): 5835-5836.
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    Ph Modulation Of Porphyrins Self-Assembly Onto Polylisine (45 visite)
    Purrello R, Bellacchio E, Gurrieri S, Lauceri R, Raudino A, Monsù Scolaro L, Santoro AM
    Journal Of Physical Chemistry B, 1998; N/D: 8852-8857.
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    Mastruzzo L, Woisard A, Ma DD, Rizzarelli E, Favre A, Le Doan T
    Photochem Photobiol (ISSN: 0031-8655), 1994 Oct; 60(4): 316-322.
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    Interaction of water-soluble porphyrins with single- and double-stranded polyribonucleotides (54 visite)
    Bustamante C, Gurrieri S, Pasternack RF, Purrello R, Rizzarelli E
    Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 1994 Sep; 34(8): 1099-1104.
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    10 Records (9 escludendo Abstract e Conferenze).
    Impact factor totale: 42.237 (38.507 escludendo Abstract e Conferenze).
    Impact factor a 5 anni totale: 45.861 (42.897 escludendo Abstract e Conferenze).

    Interrogazione bibliografica effettuata: (([btitle] "Aromatic Compounds" OR [btitle] "Binding Energy" OR [btitle] "Bioinformatics" OR [btitle] "Enzyme Kinetics" OR [btitle] "Porphyrins") AND NOT [id] = 53183)

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