The maturation mechanism of γ-glutamyl transpeptidases: Insights from the crystal structure of a precursor mimic of the enzyme from Bacillus licheniformis and from site-directed mutagenesis studies

The maturation mechanism of γ-glutamyl transpeptidases: Insights from the crystal structure of a precursor mimic of the enzyme from Bacillus licheniformis and from site-directed mutagenesis studies (111 visite)

Pica A, Chi M-C, Chen Y-Y, D'Ischia M, Lin L-L, Merlino A

Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2016; 1864(2): 195-203.

Tipo di articolo: Journal Article,

Impact factor: 5.476, Impact factor a 5 anni: 5.725

Url: https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84948453364&partnerID=40&md5=6569aec1c5036717080cf6d0ed6aa37a

Parole chiave: Autoprocessing, Single-Point Mutation, X-Ray Crystallography, γ-Glutamyltranspeptidase, γ-Gt Precursor, Alanine, Bacterial Enzyme, Carbonyl Derivative, Enzyme Precursor, Gamma Glutamyltransferase, Hydroxyl Group, Threonine, Article, Bacillus Licheniformis, Controlled Study, Crystal Structure, Enzyme Activity, Enzyme Mechanism, Enzyme Structure, Hydrogen Bond, Nonhuman, Nucleophilicity, Priority Journal, Site Directed Mutagenesis, X Ray Crystallography,

Affiliazioni:

*** IBB - CNR ***
Department of Chemical Sciences, University of Naples Federico II, Via Cintia, Naples, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone, 16, Naples, Italy
Department of Applied Chemistry, National Chiayi University, 300 Syuefu Road, Chiayi City, Taiwan

Riferimenti:

Castellano, I., Merlino, A., (2012) Cell. Mol. Life Sci., 69, pp. 3381-339

Stole, E., Smith, T.K., Manning, J.M., Meister, A., (1994) J. Biol. Chem., 269, pp. 21435-21439

Tate, S.S., Meister, A., (1981) Mol. Cell. Biochem., p. 39

Hanigan, M.H., Ricketts, W.A., (1993) Biochemistry, 32, pp. 6302-6306

Taniguchi, N., Ikeda, Y., (1998) Adv. Enzymol. Relat. Areas Mol. Biol., p. 72

Hanigan, M.H., Gallagher, B.C., Townsend, D.M., Gabarra, V., (1999) Carcinogenesis, 20, pp. 553-559

Wilk, S., Mizoguchi, H., Orlowski, M., (1978) J. Pharmacol. Exp. Ther., 206, pp. 227-232

Zhang, Q., Kulczynska, A., Webb, D.J., Megson, I.L., Botting, N.P., (2013) Chem. Commun., 49, pp. 1389-1391

Allison, R.D., (1985) Methods Enzymol., 113, pp. 419-437

Tate, S.S., (1986) FEBS Lett., 194, pp. 33-38

Inoue, M., Hiratake, J., Suzuki, H., Kumagai, H., Sakata, K., (2000) Biochemistry, 39, pp. 7764-7771

Xu, Q., Buckley, D., Guan, C., Guo, H.-C., (1999) Cell, 98, pp. 651-661

Qian, X., Guan, C., Guo, H.-C., (2003) Structure, 11, pp. 997-1003

Kim, Y., Yoon, K.-H., Khang, Y., Turley, S., Hol, W.G., (2000) Structure, 8, pp. 1059-1068

Hewitt, L., Kasche, V., Lummer, K., Lewis, R., Murshudov, G., Verma, C., Dodson, G., Wilson, K., (2000) J. Mol. Biol., 302, pp. 887-898

Ditzel, L., Huber, R., Mann, K., Heinemeyer, W., Wolf, D.H., Groll, M., (1998) J. Mol. Biol., 279, pp. 1187-1191

Bokhove, M., Yoshida, H., Hensgens, C.M., Van Der Laan, J.M., Sutherland, J.D., Dijkstra, B.W., (2010) Structure, 18, pp. 301-308

Minami, H., Suzuki, H., Kumagai, H., (2003) FEMS Microbiol. Lett., 224, pp. 169-173

Boanca, G., Sand, A., Barycki, J.J., (2006) J. Biol. Chem., 281, pp. 19029-19037

Hung, C.-P., Yang, J.-C., Chen, J.-H., Chi, M.-C., Lin, L.-L., (2011) J. Biol. Phys., 37, pp. 463-475

Murty, N.A., Tiwary, E., Sharma, R., Nair, N., Gupta, R., (2012) Enzym. Microb. Technol., 50, pp. 159-164

Chang, H.P., Liang, W.C., Lyu, R.C., Chi, M.C., Wang, T.F., Su, K.L., Hung, H.C., Lin, L.L., (2010) Biochemistry (Mosc), 75, pp. 919-929

Okada, T., Suzuki, H., Wada, K., Kumagai, H., Fukuyama, K., (2006) Proc. Natl. Acad. Sci. U. S. A., 103, pp. 6471-6476

Wada, K., Irie, M., Suzuki, H., Fukuyama, K., (2010) FEBS J., 277, pp. 1000-1009

Oinonen, C., Rouvinen, J., (2000) Protein Sci., p. 9

Lee, Y.S., Kim, H.W., Lee, K.B., Park, S.S., (2000) Biochim. Biophys. Acta, 1523, pp. 123-127

Kim, Y., Kim, S., Earnest, T.N., Hol, W.G., (2002) J. Biol. Chem., 277, pp. 2823-2829

West, M.B., Chen, Y., Wickham, S., Heroux, A., Cahill, K., Hanigan, M.H., Mooers, B.H., (2013) J. Biol. Chem., 288, pp. 31902-31913

Lin, L.L., Chen, Y.Y., Chi, M.C., Merlino, A., (2014) Biochim. Biophys. Acta, Proteins Proteomics, 1844, pp. 1523-1529

Moallic, C., Dabonne, S., Colas, B., Sine, J.P., (2006) Protein J., 25, pp. 391-397

Castellano, I., Di Salle, A., Merlino, A., Rossi, M., Laara, F., (2011) Extremophiles, 15, pp. 259-270

Chi, M.C., Lo, Y.H., Chen, Y.Y., Lin, L.L., Merlino, A., (2014) Biochim. Biophys. Acta, Proteins Proteomics, 1844, pp. 2290-2297

Lyu, R.C., Hu, H.Y., Kuo, L.Y., Lo, H.F., Ong, P.L., Chang, H.P., Lin, L.L., (2009) Curr. Microbiol., 59, pp. 101-106

Otwinowski, Z., Minor, W., (1997) Methods in Enzymology, 276, pp. 307-326. , ed. J. Charles W. Carter, Academic Press

Emsley, P., Cowtan, K., (2004) Acta Crystallogr. Sect. D: Biol. Crystallogr., 60, pp. 2126-2132

Adams, P.D., Afonine, P.V., Bunkoczi, G., Chen, V.B., Davis, I.W., Echols, N., Headd, J.J., Zwart, P.H., (2010) Acta Crystallogr. Sect. D: Biol. Crystallogr., 66, pp. 213-221

Noren, C.J., Wang, J., Perler, F.B., (2000) Angew. Chem., 39, pp. 450-466

Chi, M.C., Chen, Y.Y., Lo, H.F., Lin, L.L., (2012) FEBS Open Bio, 2, pp. 298-304

Hashimoto, W., Suzuki, H., Nohara, S., Kumagai, H., (1992) Biochem. Biophys. Res. Commun., 189, pp. 173-178

γ-Glutamyl transpeptidases (γ-GTs) are members of N-terminal nucleophile hydrolase superfamily. They are synthetized as single-chain precursors, which are then cleaved to form mature enzymes. Basic aspects of autocatalytic processing of these pro-enzymes are still unknown. Here we describe the X-ray structure of the precursor mimic of Bacillus licheniformis γ-GT (BlGT), obtained by mutating catalytically important threonine to alanine (T399A-BlGT), and report results of autoprocessing of mutants of His401, Thr415, Thr417, Glu419 and Arg571. Data suggest that Thr417 is in a competent position to activate the catalytic threonine (Thr399) for nucleophilic attack of the scissile peptide bond and that Thr415 plays a major role in assisting the process. On the basis of these new structural results, a possible mechanism of autoprocessing is proposed. This mechanism, which guesses the existence of a six-membered transition state involving one carbonyl and two hydroxyl groups, is in agreement with all the available experimental data collected on γ-GTs from different species and with our new Ala-scanning mutagenesis data. © 2015 Elsevier B.V. All rights reserved.

Interrogazione effettuata: [atitle, keywords] "Autoprocessing" OR [atitle, keywords] "Single-Point Mutation" OR [atitle, keywords] "X-Ray Crystallography" OR [atitle, keywords] "γ-Glutamyltranspeptidase" OR [atitle, keywords] "γ-Gt Precursor" OR [atitle, keywords] "Bacterial Enzyme" OR [atitle, keywords] "Carbonyl Derivative"

Nessun risultato.

Interrogazione effettuata: [ptitle] "Autoprocessing" OR [ptitle] "Single-Point Mutation" OR [ptitle] "X-Ray Crystallography" OR [ptitle] "γ-Glutamyltranspeptidase" OR [ptitle] "γ-Gt Precursor" OR [ptitle] "Bacterial Enzyme" OR [ptitle] "Carbonyl Derivative"

Nessun risultato.

Interrogazione effettuata: [btitle, keywords] "Autoprocessing" [btitle, keywords] "Single-Point Mutation" [btitle, keywords] "X-Ray Crystallography" [btitle, keywords] "γ-Glutamyltranspeptidase" [btitle, keywords] "γ-Gt Precursor" [btitle, keywords] "Bacterial Enzyme" [btitle, keywords] "Carbonyl Derivative"

Cerca bibliografia collegata su PubMed

Ricerca avanzata▼

Vai a fine pagina Versione per copia e incolla facile Esporta tutto in BibTeX Esporta tutto in EndNote

Esposito L, Donnarumma F, Ruggiero A, Leone S, Vitagliano L, Picone D
* Structure, stability and aggregation propensity of a Ribonuclease A-Onconase chimera (51 visite)
Int J Biol Macromol (ISSN: 0141-8130linking, 1879-0003electronic), 2019 Jul 15; 133: 1125-1133.
Impact Factor: 4.784
Dettagli    Esporta in BibTeX    Esporta in EndNote

Buemi MR, Di Fiore A, De Luca L, Angeli A, Mancuso F, Ferro S, Monti SM, Buonanno M, Russo E, De Sarro G, De Simone G, Supuran CT, Gitto R
* Exploring structural properties of potent human carbonic anhydrase inhibitors bearing a 4-(cycloalkylamino-1-carbonyl)benzenesulfonamide moiety (47 visite)
Eur J Med Chem (ISSN: 1768-3254electronic, 0223-5234linking), 2019 Feb 1; 163: 443-452.
Impact Factor: 2.882
Dettagli    Esporta in BibTeX    Esporta in EndNote

Alterio V, Esposito D, Monti SM, Supuran CT, De Simone G
* Crystal structure of the human carbonic anhydrase II adduct with 1-(4-sulfamoylphenyl-ethyl)-2, 4, 6-triphenylpyridinium perchlorate, a membrane-impermeant, isoform selective inhibitor (287 visite)
J Enzym Inhib Med Ch (ISSN: 1475-6374electronic, 1475-6366linking), 2018 Dec; 33(1): 151-157.
Impact Factor: 4.293
Dettagli    Esporta in BibTeX    Esporta in EndNote

Merlino A, Marzo T, Messori L
* Protein Metalation by Anticancer Metallodrugs: A Joint ESI MS and XRD Investigative Strategy (62 visite)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2017 May 23; 23(29): 6942-6947.
Impact Factor: 5.16
Dettagli    Esporta in BibTeX    Esporta in EndNote

Vullo D, Supuran CT, Scozzafava A, De Simone G, Monti SM, Alterio V, Carta F
* Kinetic and X-ray crystallographic investigations of substituted 2-thio-6-oxo-1, 6-dihydropyrimidine-benzenesulfonamides acting as carbonic anhydrase inhibitors (97 visite)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391), 2016 Aug 15; 24(16): 3643-3648.
Impact Factor: 2.93
Dettagli    Esporta in BibTeX    Esporta in EndNote

Nikitushkin VD, Demina GR, Shleeva MO, Guryanova SV, Ruggiero A, Berisio R, Kaprelyants AS
* A product of RpfB and RipA joint enzymatic action promotes the resuscitation of dormant mycobacteria (197 visite)
Febs J (ISSN: 1742-464x), 2015 Apr 3; 282(13): 2500-2511.
Impact Factor: 4.237
Dettagli    Esporta in BibTeX    Esporta in EndNote

De Simone G, Di Fiore A, Capasso C, Supuran CT
* The zinc coordination pattern in the eta-carbonic anhydrase from Plasmodium falciparum is different from all other carbonic anhydrase genetic families (257 visite)
Bioorg Med Chem Lett (ISSN: 0960-894x), 2015 Apr 1; 25(7): 1385-1389.
Impact Factor: 2.486
Dettagli    Esporta in BibTeX    Esporta in EndNote

Berisio R, Squeglia F, Ruggiero A, Petraccone L, Stellato MI, Del Vecchio P
* Differential thermodynamic behaviours of the extra-cellular regions of two Ser/Thr PrkC kinases revealed by calorimetric studies (126 visite)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2015 Feb 8; 1854(5): 402-409.
Impact Factor: 5.158
Dettagli    Esporta in BibTeX    Esporta in EndNote

Ceruso M, Carta F, Osman SM, Alothman Z, Monti SM, Supuran CT
* Inhibition studies of bacterial, fungal and protozoan β-class carbonic anhydrases with Schiff bases incorporating sulfonamide moieties (147 visite)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391), 2015; 23(15): 4181-4187.
Impact Factor: 2.923
Dettagli    Esporta in BibTeX    Esporta in EndNote

Messori L, Marzo T, Merlino A
* Interactions of carboplatin and oxaliplatin with proteins: Insights from X-ray structures and mass spectrometry studies of their ribonuclease A adducts (107 visite)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2015; N/D: N/D-N/D.
Impact Factor: 3.205
Dettagli    Esporta in BibTeX    Esporta in EndNote

D'Ambrosio K, Lopez M, Dathan NA, Ouahrani-bettache S, Köhler S, Ascione G, Monti SM, Winum JY, De Simone G
* Structural basis for the rational design of new anti-Brucella agents: The crystal structure of the C366S mutant of l-histidinol dehydrogenase from Brucella suis (172 visite)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Feb; 97(1): 114-120.
Impact Factor: 2.963
Dettagli    Esporta in BibTeX    Esporta in EndNote

Chi M-C, Lo Y-H, Chen Y-Y, Lin L-L, Merlino A
* γ-Glutamyl transpeptidase architecture: Effect of extra sequence deletion on autoprocessing, structure and stability of the protein from Bacillus licheniformis (127 visite)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014; 1844(12): 2290-2297.
Impact Factor: 4.882
Dettagli    Esporta in BibTeX    Esporta in EndNote

Lin L-L, Chen Y-Y, Chi M-C, Merlino A
* Low resolution X-ray structure of γ-glutamyltranspeptidase from Bacillus licheniformis: Opened active site cleft and a cluster of acid residues potentially involved in the recognition of a metal ion (131 visite)
Bba-Gen Subjects (ISSN: 0925-4439, 0006-3002, 1570-9639), 2014; 1844(9): 1523-1529.
Impact Factor: 4.882
Dettagli    Esporta in BibTeX    Esporta in EndNote

Alterio V, Pan P, Parkkila S, Buonanno M, Supuran CT, Monti SM, De Simone G
* The Structural Comparison Between Membrane-Associated Human Carbonic Anhydrases Provides Insights Into Drug Design Of Selective Inhibitors (149 visite)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2014; 101(7): 769-778.
Impact Factor: 2.385
Dettagli    Esporta in BibTeX    Esporta in EndNote

Ronda L, Merlino A, Bettati S, Verde C, Balsamo A, Mazzarella L, Mozzarelli A, Vergara A
* Role of tertiary structures on the Root effect in fish hemoglobins (160 visite)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2013 Sep; 1834(9): 1885-1893.
Impact Factor: 3.191
Dettagli    Esporta in BibTeX    Esporta in EndNote

De Simone G, Alterio V, Supuran CT
* Exploiting the hydrophobic and hydrophilic binding sites for designing carbonic anhydrase inhibitors (189 visite)
Expert Opin Drug Discov (ISSN: 1746-0441), 2013 Jul; 8(7): 793-810.
Impact Factor: 3.467
Dettagli    Esporta in BibTeX    Esporta in EndNote

De Simone G, Supuran CT
* (In)organic anions as carbonic anhydrase inhibitors (166 visite)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2012 Jun; 111: 117-129.
Impact Factor: 3.197
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pica A, Russo Krauss I, Castellano I, Rossi M, La Cara F, Graziano G, Sica F, Merlino A
* Exploring The Unfolding Mechanism Of Gamma-Glutamyltranspeptidases: The Case Of The Thermophilic Enzyme From Geobacillus Thermodenitrificans (156 visite)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2012 Apr; 1824(4): 571-577.
Impact Factor: 3.733
Dettagli    Esporta in BibTeX    Esporta in EndNote

Russo Krauss I, Sica F, Mattia CA, Merlino A
* Increasing the X-ray diffraction power of protein crystals by dehydration: the case of bovine serum albumin and a survey of literature data (158 visite)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067electronic), 2012 Mar; 13(3): 3782-3800.
Impact Factor: 2.464
Dettagli    Esporta in BibTeX    Esporta in EndNote

Esposito L, Ruggiero A, Masullo M, Ruocco MR, Lamberti A, Arcari P, Zagari A, Vitagliano L
* Crystallographic and spectroscopic characterizations of Sulfolobus solfataricus TrxA1 provide insights into the determinants of thioredoxin fold stability (144 visite)
J Struct Biol (ISSN: 1047-8477), 2012 Feb; 177(2): 506-512.
Impact Factor: 3.361
Dettagli    Esporta in BibTeX    Esporta in EndNote

Castellano I, Merlino A
* γ-glutamyltranspeptidases: Sequence, structure, biochemical properties, and biotechnological applications (129 visite)
Cell Mol Life Sci (ISSN: 1420-682x), 2012; 69(20): 3381-3394.
Impact Factor: 5.615
Dettagli    Esporta in BibTeX    Esporta in EndNote

Squeglia F, Marchetti R, Ruggiero A, Lanzetta R, Marasco D, Dworkin J, Petoukhov M, Molinaro A, Berisio R, Silipo A
* Chemical basis of peptidoglycan discrimination by PrkC, a key kinase involved in bacterial resuscitation from dormancy (138 visite)
J Am Chem Soc (ISSN: 0002-7863, 0002-2786, 1520-5126), 2011 Dec 28; 133(51): 20676-20679.
Impact Factor: 9.907
Dettagli    Esporta in BibTeX    Esporta in EndNote

Merlino A, Russo Krauss I, Albino A, Pica A, Vergara A, Masullo M, De Vendittis E, Sica F
* Improving Protein Crystal Quality by the Without-Oil Microbatch Method: Crystallization and Preliminary X-ray Diffraction Analysis of Glutathione Synthetase from Pseudoalteromonas haloplanktis (149 visite)
Int J Mol Sc (ISSN: 1422-0067, 1661-6596, 1422-0067electronic), 2011 Sep; 12(9): 6312-6319.
Impact Factor: 2.598
Dettagli    Esporta in BibTeX    Esporta in EndNote

Ruggiero A, Squeglia F, Marasco D, Marchetti R, Molinaro A, Berisio R
* X-ray structural studies of the entire extracellular region of the serine/threonine kinase PrkC from Staphylococcus aureus (142 visite)
Biochem J (ISSN: 0264-6021, 1470-8728electronic, 0264-6021linking), 2011 Apr 1; 435(1): 33-41.
Impact Factor: 4.897
Dettagli    Esporta in BibTeX    Esporta in EndNote

Castellano I, Di Salle A, Merlino A, Rossi M, La Cara F
* Gene cloning and protein expression of γ-glutamyltranspeptidases from Thermus thermophilus and Deinococcus radiodurans: Comparison of molecular and structural properties with mesophilic counterparts (102 visite)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2011; 15(2): 259-270.
Impact Factor: 2.941
Dettagli    Esporta in BibTeX    Esporta in EndNote

Merlino A, Russo Krauss I, Castellano I, De Vendittis E, Rossi B, Conte M, Vergara A, Sica F
* Structure and flexibility in cold-adapted iron superoxide dismutases: The case of the enzyme isolated from Pseudoalteromonas haloplanktis (175 visite)
J Struct Biol (ISSN: 1047-8477), 2010 Dec; 172(3): 343-352.
Impact Factor: 3.5
Dettagli    Esporta in BibTeX    Esporta in EndNote

Ruggiero A, Marasco D, Squeglia F, Soldini S, Pedone E, Pedone C, Berisio R
* Structure and functional regulation of RipA, a mycobacterial enzyme essential for daughter cell separation (147 visite)
Structure (ISSN: 0969-2126, 1878-4186), 2010 Sep 8; 18(9): 1184-1190.
Impact Factor: 6.337
Dettagli    Esporta in BibTeX    Esporta in EndNote

Di Fiore A, Monti SM, Innocenti A, Winum J-Y, De Simone G, Supuran CT
* Carbonic anhydrase inhibitors: Crystallographic and solution binding studies for the interaction of a boron-containing aromatic sulfamide with mammalian isoforms I-XV (115 visite)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2010 Jun 15; 20(12): 3601-3605.
Impact Factor: 2.661
Dettagli    Esporta in BibTeX    Esporta in EndNote

De Simone G, Supuran CT
* Carbonic anhydrase IX: Biochemical and crystallographic characterization of a novel antitumor target (172 visite)
Bba-Mol Basis Dis (ISSN: 1570-9639, 0006-3002, 0925-4439), 2010 Feb; 1804(2): 404-409.
Impact Factor: 2.773
Dettagli    Esporta in BibTeX    Esporta in EndNote

Guler OO, De Simone G, Supuran CT
* Drug design studies of the novel antitumor targets carbonic anhydrase IX and XII (214 visite)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2010; 17(15): 1516-1526.
Impact Factor: 4.63
Dettagli    Esporta in BibTeX    Esporta in EndNote

Merlino A, Vitagliano L, Howes BD, Verde C, Di Prisco G, Smulevich G, Sica F, Vergara A
* Combined Crystallographic and Spectroscopic Analysis of Trematomus bernacchii Hemoglobin Highlights Analogies and Differences in the Peculiar Oxidation Pathway of Antarctic Fish Hemoglobins (144 visite)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2009 Dec; 91(12): 1117-1125.
Impact Factor: 2.605
Dettagli    Esporta in BibTeX    Esporta in EndNote

Ruggiero A, Masullo M, Marasco D, Ruocco MR, Grimaldi P, Arcari P, Zagari A, Vitagliano L
* The dimeric structure of Sulfolobus solfataricus thioredoxin A2 and the basis of its thermostability (121 visite)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2009 Dec; 77(4): 1004-1008.
Impact Factor: 3.085
Dettagli    Esporta in BibTeX    Esporta in EndNote

Bellia F, Calabrese V, Guarino F, Cavallaro M, Cornelius C, De Pinto V, Rizzarelli E
* Carnosinase levels in aging brain: redox state induction and cellular stress response (165 visite)
Antioxid Redox Signal (ISSN: 1557-7716, 1523-0864), 2009 Nov; 11(11): 2759-2775.
Impact Factor: 7.581
Dettagli    Esporta in BibTeX    Esporta in EndNote

Wekselman I, Davidovich C, Agmon I, Zimmerman E, Rozenberg H, Bashan A, Berisio R, Yonath A
* Ribosome's mode of function: Myths, facts and recent results (127 visite)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617print), 2009 Mar; 15(3): 122-130.
Impact Factor: 1.807
Dettagli    Esporta in BibTeX    Esporta in EndNote

Limauro D, Saviano M, Galdi I, Rossi M, Bartolucci S, Pedone E
* Sulfolobus solfataricus protein disulphide oxidoreductase: insight into the roles of its redox sites (92 visite)
Protein Engineering Design & Selection (ISSN: 1741-0126), 2009 Jan; 22(1): 19-26.
Impact Factor: 2.596
Dettagli    Esporta in BibTeX    Esporta in EndNote

Alterio V, Di Fiore A, D'Ambrosio K, Supuran CT, De Simone G
* X-Ray Crystallography of Carbonic Anhydrase Inhibitors and Its Importance in Drug Design (142 visite)
Drug Des Of Zinc Enzyme Inhibitors (ISSN: 9780-4702, 9780-470275009), 2009; N/D: 73-138.
Impact Factor: 1.156
Dettagli    Esporta in BibTeX    Esporta in EndNote

Merlino A, Sica F, Mazzarella L, Zagari A, Vergara A
* Correlation between Raman and X-ray crystallography data of (Pro-Pro-Gly)(10) (129 visite)
Biophys Chem (ISSN: 0301-4622, 0301-4622linking), 2008 Sep; 137(1): 24-27.
Impact Factor: 2.362
Dettagli    Esporta in BibTeX    Esporta in EndNote

Prato S, Vitale RM, Contursi P, Lipps G, Saviano M, Rossi M, Bartolucci S
* Molecular modeling and functional characterization of the monomeric primase-polymerase domain from the Sulfolobus solfataricus plasmid pIT3 (148 visite)
Febs J (ISSN: 1742-464x), 2008; 275(17): 4389-4402.
Impact Factor: 3.139
Dettagli    Esporta in BibTeX    Esporta in EndNote

Alterio V, De Simone G, Monti SM, Scozzafava A, Supuran CT
* Carbonic anhydrase inhibitors: Inhibition of human, bacterial, and archaeal isozymes with benzene-1, 3-disulfonamides-Solution and crystallographic studies (138 visite)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2007 Sep 1; 17(15): 4201-4207.
Impact Factor: 2.604
Dettagli    Esporta in BibTeX    Esporta in EndNote

Di Fiore A, Scozzafava A, Winum JY, Montero JL, Pedone C, Supuran CT, De Simone G
* Carbonic anhydrase inhibitors: binding of an antiglaucoma glycosyl-sulfanilamide derivative to human isoform II and its consequences for the drug design of enzyme inhibitors incorporating sugar moieties (140 visite)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2007 Mar 15; 17(6): 1726-1731.
Impact Factor: 2.604
Dettagli    Esporta in BibTeX    Esporta in EndNote

Menchise V, De Simone G, Di Fiore A, Scozzafava A, Supuran CT
* Carbonic anhydrase inhibitors: X-ray crystallographic studies for the binding of 5-amino-1, 3, 4-thiadiazole-2-sulfonamide and 5-(4-amino-3-chloro-5-fluorophenylsulfonamido)1, 3, 4-thiadiazole-2-sulfon amide to human isoform II (123 visite)
Bioorganic & Medicinal Chemistry Letters (ISSN: 0960-894x), 2006 Dec 15; 16(24): 6204-6208.
Impact Factor: 2.538
Dettagli    Esporta in BibTeX    Esporta in EndNote

Di Fiore A, Pedone C, D'Ambrosio K, Scozzafava A, De Simone G, Supuran CT
* Carbonic anhydrase inhibitors: Valdecoxib binds to a different active site region of the human isoform II as compared to the structurally related cyclooxygenase II 'selective' inhibitor celecoxib (137 visite)
Bioorg Med Chem Lett Bioorganic And Medicinal Chemistry Letters (ISSN: 0960-894x), 2006 Jan 15; 16(2): 437-442.
Impact Factor: 2.538
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, Saviano M, Bartolucci S, Rossi M, Ausili A, Scire A, Bertoli E, Tanfani F
* Temperature-, SDS-, and pH-induced conformational changes in protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus: A dynamic simulation and fourier transform infrared spectroscopic study (104 visite)
J Proteome Res (ISSN: 1535-3893), 2005 Nov; 4(6): 1972-1980.
Impact Factor: 6.901
Dettagli    Esporta in BibTeX    Esporta in EndNote

Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal structure of a ternary complex of the alcohol dehydrogenase from Sulfolobus solfataricus (137 visite)
Biochemistry (ISSN: 0006-2960, 1520-4995, 1520-4995electronic), 2003 Dec 16; 42(49): 14397-14407.
Impact Factor: 3.922
Dettagli    Esporta in BibTeX    Esporta in EndNote

Esposito L, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A
* Crystal Structure Of The Alcohol Dehydrogenase From The Hyperthermophilic Archaeon Sulfolobus Solfataricus At 1. 85 Angstrom Resolution (134 visite)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2002 Apr 26; 318(2): 463-477.
Impact Factor: 5.359
Dettagli    Esporta in BibTeX    Esporta in EndNote

Esposito L, Vitagliano L, Zagari A, Mazzarella L
Pyramidalization of backbone carbonyl carbon atoms in proteins (135 visite)
Protein Sci (ISSN: 0961-8368, 1469-896xelectronic), 2000 Oct; 9(10): 2038-2042.
Impact Factor: 3.869
Dettagli    Esporta in BibTeX    Esporta in EndNote

46 Records (46 escludendo Abstract e Conferenze).
Impact factor totale: 173.108 (173.108 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 164.309 (164.309 escludendo Abstract e Conferenze).

Versione per copia e incolla facile Esporta tutto in BibTeX Esporta tutto in EndNote

Vai a inizio pagina

Esporta in BibTeX Esporta in EndNote

Copyright 2019 © CNR Istituto di Biostrutture e Bioimmagini