Home Ricerca Sedi Chi siamo Organigramma Personale Contatti Didattica Login Documenti EN
Progetti in evidenza
(Link esterno)

(1204 visite)

Fondo Europeo Pesca
(614 visite)

(Link esterno)

Fondazione Veronesi
(Link esterno)

(1082 visite)

(1179 visite)

(Link esterno)

(1320 visite)

(1083 visite)

(1025 visite)

MFAG Grant
(1126 visite)

(Link esterno)


Contiene: [X]      Estesa     Autori: [X]  Tipo di lavoro: [X]
Data iniziale: Data finale: [X]      Sede: Affiliazione IBB     
[Pulisci modulo]

ATOX1 gene silencing increases susceptibility to anticancer therapy based on copper ionophores or chelating drugs (435 visite)

Barresi V, Spampinato G, Musso N, Trovato Salinaro A, Rizzarelli E, Condorelli DF

J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2016; 156: 145-152.

Tipo di articolo: Journal Article, , Impact factor: 3.348, Impact factor a 5 anni: 3.207, Url: https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84954202975&partnerID=40&md5=b452999b8983a065457be0c9911b8325

Parole chiave: Atox1, Chelating Drugs, Colon Cancer, Copper, Ionophores, Adenosine Triphosphatase, Chaperone, Chelating Agent, Cloxiquine, Copper Chelating Agent, Copper Ion, Copper Ionophore, Tetrakis(2 Pyridylmethyl)ethylenediamine, Peroxiredoxin 3, Unclassified Drug, Article, Caco 2 Cell Line, Cell Level, Controlled Study, Copper Protein Binding, Cytosol, Cytotoxicity, Drug Sensitivity, Gene Silencing, Homeostasis, Human, Human Cell, In Vitro Study, Intracellular Transport, Protein Depletion, Protein Expression, Protein Function, Rna Interference, Trans Golgi Network,

Affiliazioni: *** IBB - CNR ***
Department of Biomedical and Biotechnological Sciences, Unit of Medical Biochemistry, University of Catania, Via S. Sofia 64, Catania, Italy
Institute of Biostructures and Bioimaging, National Council of Research, UOS Catania, Italy

Riferimenti: Festa, R.A., Thiele, D.J., Copper: An essential metal in biology (2011) Curr. Biol., 21 (21), pp. R877-R883. ,

Lutsenko, S., Human copper homeostasis: A network of interconnected pathways (2010) Curr. Opin. Chem. Biol., 14 (2), pp. 211-217

Robinson, N.J., Winge, D.R., Copper metallochaperones (2010) Annu. Rev. Biochem., 79, pp. 537-562

Harrison, M.D., Jones, C.E., Solioz, M., Dameron, C.T., Intracellular copper routing: The role of copper chaperones (2000) Trends Biochem. Sci., 25, pp. 29-32

Ding, W.Q., Liu, B., Vaught, J.L., Anticancer activity of the antibiotic clioquinol (2005) Cancer Res., 65, pp. 3389-3395

Ding, W.Q., Lind, S.E., Metal ionophores - An emerging class of anticancer drugs (2009) IUBMB Life, 61 (11), pp. 1013-1018

Margalioth, E.J., Schenker, J.G., Chevion, M., Copper and zinc levels in normal and malignant tissues (1983) Cancer, 52 (5), pp. 868-872. , (1)

Dìez, M., Cerdàn, F.J., Arroyo, M., Balibrea, J.L., Use of the copper/zinc ratio in the diagnosis of lung cancer (1989) Cancer, 63 (4), pp. 726-730. , (15)

Mao, X., Li, X., Sprangers, R., Wang, X., Venugopal, A., Wood, T., Zhang, Y., Schimmer, A.D., Clioquinol inhibits the proteasome and displays preclinical activity in leukemia and myeloma (2009) Leukemia, 23, pp. 585-590

Mao, X., Schimmer, A.D., The toxicology of clioquinol (2008) Toxicol. Lett., 182, pp. 1-6

Chen, D., Cui, Q.C., Yang, H., Barrea, R.A., Sarkar, F.H., Sheng, S., Yan, B., Dou, Q.P., Clioquinol, a therapeutic agent for Alzheimer's disease, has proteasome-inhibitory, androgen receptor suppressing, apoptosis-inducing, and antitumor activities in human prostate cancer cells and xenografts (2007) Cancer Res., 67, pp. 1636-1644

Daniel, K.G., Chen, D., Orlu, S., Cui, Q.C., Miller, F.R., Dou, Q.P., Clioquinol and pyrrolidinedithiocarbamate complex with copper to form proteasome inhibitors and apoptosis inducers in human breast cancer cells (2005) Breast Cancer Res., 7 (6), pp. R897-R908

Cao, B., Li, J., Zhu, J., Shen, M., Han, K., Zhang, Z., Yu, Y., Mao, X., The antiparasitic clioquinol induces apoptosis in leukemia and myeloma cells by inhibiting histone deacetylase activity (2013) J. Biol. Chem., 288 (47), pp. 34181-34189. , 22

Zhai, S., Yang, L., Cui, Q.C., Sun, Y., Dou, Q.P., Yan, B., Tumor cellular proteasome inhibition and growth suppression by 8-hydroxyquinoline and clioquinol requires their capabilities to bind copper and transport copper into cells (2010) J. Biol. Inorg. Chem., 15 (2), pp. 259-269

Sperandio, S., De Belle, I., Bredesen, D.E., An alternative, nonapoptotic form of programmed cell death (2000) Proc. Natl. Acad. Sci. U. S. A., 97, p. 14376

Tardito, S., Barilli, A., Bassanetti, I., Tegoni, M., Bussolati, O., Franchi-Gazzola, R., Mucchino, C., Marchiò, L., Copper-dependent cytotoxicity of 8-hydroxyquinoline derivatives correlates with their hydrophobicity and does not require caspase activation (2012) J. Med. Chem., 55, pp. 10448-10459

Carraway, R.E., Dobner, P.R., Zinc pyrithione induces ERK- and PKC-dependent necrosis distinct from TPEN-induced apoptosis in prostate cancer cells (2012) Biochim. Biophys. Acta, 1823 (2), pp. 544-557

Pang, W., Leng, X., Lu, H., Yang, H., Song, N., Tan, L., Jiang, Y., Guo, C., Depletion of intracellular zinc induces apoptosis of cultured hippocampal neurons through suppression of ERK signaling pathway and activation of caspase-3 (2013) Neurosci. Lett., 552, pp. 140-145. , 27

Hyun, H.J., Sohn, J.H., Ha, D.W., Ahn, Y.H., Koh, J.Y., Yoon, Y.H., Depletion of intracellular zinc and copper with TPEN results in apoptosis of cultured human retinal pigment epithelial cells (2001) Invest. Ophthalmol. Vis. Sci., 42 (2)

Klomp, L.W., Lin, S.J., Yuan, D.S., Klausner, R.D., Culotta, V.C., Gitlin, J.D., Identification and functional expression of HAH1, a novel human gene involved in copper homeostasis (1997) J. Biol. Chem., 272 (14), pp. 9221-9226. , 4

Hamza, I., Prohaska, J., Gitlin, J.D., Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase (2003) Proc. Natl. Acad. Sci. U. S. A., 100 (3), pp. 1215-1220. , 4

Miyayama, T., Suzuki, K.T., Ogra, Y., Copper accumulation and compartmentalization in mouse fibroblast lacking metallothionein and copper chaperone. Atox1 (2009) Toxicol. Appl. Pharmacol., 237 (2), pp. 205-213

McRae, R., Lai, B., Fahrni, C.J., Copper redistribution in Atox1-deficient mouse fibroblast cells (2010) J. Biol. Inorg. Chem., 15 (1), pp. 99-105

Barresi, V., Palumbo, G.A., Musso, N., Consoli, C., Capizzi, C., Meli, C.R., Romano, A., Condorelli, D.F., Clonal selection of 11q CN-LOH and CBL gene mutation in a serially studied patient during MDS progression to AML (2010) Leuk. Res., 34 (11), pp. 1539-1542

Barresi, V., Romano, A., Musso, N., Capizzi, C., Consoli, C., Martelli, M.P., Palumbo, G.A., Condorelli, D.F., Broad copy neutral-loss of heterozygosity regions and rare recurring copy number abnormalities in normal karyotype-acute myeloid leukemia genomes (2010) Genes Chromosom. Cancer, 49 (11), pp. 1014-1023

Irizarry, R.A., Hobbs, B., Collin, F., Beazer-Barclay, Y.D., Antonellis, K.J., Scherf, U., Speed, T.P., Exploration, normalization, and summaries of high density oligonucleotide array probe level data (2003) Biostatistics, 4 (2), pp. 249-264

Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J. Immunol. Methods, 65, pp. 55-63. , 55

Scherf, U., Ross, D.T., Waltham, M., Smith, L.H., Lee, J.K., Tanabe, L., Kohn, K.W., Weinstein, J.N., A gene expression database for the molecular pharmacology of cancer (2000) Nat. Genet., 24 (3), pp. 236-244

Livak, K.J., Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2(- delta delta C(T)) method (2001) Methods, 25 (4), pp. 402-408

Barresi, V., Ragusa, A., Fichera, M., Musso, N., Castiglia, L., Rappazzo, G., Travali, S., Condorelli, D.F., Decreased expression of GRAF1/OPHN-1-L in the X-linked alpha thalassemia mental retardation syndrome (2010) BMC Med. Genet., 3, p. 28. , 6

Sheffer, M., Bacolod, M.D., Zuk, O., Giardina, S.F., Pincas, H., Barany, F., Paty, P.B., Domany, E., Association of survival and disease progression with chromosomal instability: A genomic exploration of colorectal cancer (2009) Proc. Natl. Acad. Sci. U. S. A., 106 (17), pp. 7131-7136. , 28

Lee, J., Peña, M.M., Nose, Y., Thiele, D.J., Biochemical characterization of the human copper transporter Ctr1 (2002) J. Biol. Chem., 277 (6), pp. 4380-4387

Itoh, S., Kim, H.W., Nakagawa, O., Ozumi, K., Lessner, S.M., Aoki, H., Akram, K., Fukai, T., Novel role of antioxidant-1 (Atox1) as a copper-dependent transcription factor involved in cell proliferation (2008) J. Biol. Chem., 283 (14), pp. 9157-9167. , 4

Muller, P.A., Klomp, L.W., ATOX1: A novel copper-responsive transcription factor in mammals? (2009) Int. J. Biochem. Cell Biol., 41, pp. 1233-1236

Fatfat, M., Merhi, R.A., Rahal, O., Stoyanovsky, D.A., Zaki, A., Haidar, H., Kagan, V.E., Machaca, K., Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species (2014) BMC Cancer, 14, p. 527. , 21

Safaei, R., Maktabi, M.H., Blair, B.G., Larson, C.A., Howell, S.B., Effects of the loss of Atox1 on the cellular pharmacology of cisplatin (2009) J. Inorg. Biochem., 103, pp. 333-341

Van Den Berghe, P.V., Folmer, D.E., Malingre, H.E., Van Beurden, E., Klomp, A.E., Van De Sluis, B., Merkx, M., Klomp, L.W., Human copper transporter 2 is localized in late endosomes and lysosomes and facilitates cellular copper uptake (2007) Biochem. J., 407, pp. 49-59

Milardi, D., Rizzarelli, E., Neurodegeneration: Metallostasis and proteostasis (2011) R. Chem. Soc.

Fulda, S., Vucic, D., Targeting IAP proteins for therapeutic intervention in cancer (2012) Nat. Rev. Drug Discov., (11), pp. 109-124

Cater, M.A., Haupt, Y., Clioquinol induces cytoplasmic clearance of the X-linked inhibitor of apoptosis protein (XIAP): Therapeutic indication for prostate cancer (2011) Biochem. J., 436, pp. 481-491

Yoshizawa, K., Nozaki, S., Kitahara, H., Ohara, T., Kato, K., Kawashiri, S., Yamamoto, E., Copper efflux transporter (ATP7B) contributes to the acquisition of cisplatin-resistance in human oral squamous cell lines (2007) Oncol. Rep., 18, pp. 987-991

Ishida, S., Lee, J., Thiele, D.J., Herskowitz, I., Uptake of the anticancer drug cisplatin mediated by the copper transporter Ctr1 in yeast and mammals (2002) Proc. Natl. Acad. Sci. U. S. A., 99, pp. 14298-14302

Kelner, G.S., Lee, M., Clark, M.E., Maciejewski, D., McGrath, D., Rabizadeh, S., Lyons, T., Maki, R.A., The copper transport protein Atox1 promotes neuronal survival (2000) J. Biol. Chem., 275, pp. 580-584

Hatori, Y., Clasen, S., Hasan, N.M., Barry, A.N., Lutsenko, S., Functional partnership of the copper export machinery and glutathione balance in human cells (2012) J. Biol. Chem., 287, pp. 26678-26687

Jeney, V., Itoh, S., Wendt, M., Gradek, Q., Ushio-Fukai, M., Harrison, D.G., Fukai, T., Role of antioxidant-1 in extracellular superoxide dismutase function and expression (2005) Circ. Res., 96, pp. 723-729

Hatori, Y., Lutsenko, S., An expanding range of functions for the copper chaperone/antioxidant protein Atox1 (2013) Antioxid. Redox Signal., 19, pp. 945-957

Burkhead, J.L., Ralle, M., Wilmarth, P., David, L., Lutsenko, S., Elevated copper remodels hepatic RNA processing machinery in the mouse model of Wilson disease (2011) J. Mol. Biol., 406, pp. 44-58

Suzuki, K.T., Someya, A., Komada, Y., Ogra, Y., Roles of metallothionein in copper homeostasis: Responses to copper-deficient diets in mice (2002) J. Inorg. Biochem., 88, pp. 173-182

Nartey, N.O., Frei, J.V., Cherian, M.G., Hepatic copper and metallothionein distribution in Wilson disease (hepatolenticular degeneration) (1987) Lab. Investig., 57, pp. 397-401

Makhov, P., Golovine, K., Uzzo, R.G., Rothman, J., Crispen, P.L., Shaw, T., Scoll, B.J., Kolenko, V.M., Zinc chelation induces rapid depletion of the X-linked inhibitor of apoptosis (XIAP) and sensitizes prostate cancer cells to TRAIL-mediated apoptosis (2008) Cell Death Differ., 15 (11), pp. 1745-1751

Nakatani, T., Tawaramoto, M., Opare, K.D., Kojima, A., Matsui-Yuasa, I., Apoptosis induced by chelation of intracellular zinc is associated with depletion of cellular reduced glutathione level in rat hepatocytes (2000) Chem. Biol. Interact., 125, pp. 151-163

Copper is a catalytic cofactor required for the normal function of many enzymes involved in fundamental biological processes but highly cytotoxic when in excess. Therefore its homeostasis and distribution is strictly regulated by a network of transporters and intracellular chaperones. ATOX1 (antioxidant protein 1) is a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network. In the present study the Caco-2 cell line, a colon carcinoma cell line, was used as an in vitro model to evaluate if ATOX1 deficiency could affect sensitivity to experimentally induced copper dyshomeostasis. Silencing of ATOX1 increased toxicity of a short treatment with a high concentration of Cu2 +. Copper ionophores, such as 5-chloro-8-hydroxyquinoline, induced a copper-dependent cell toxicity which was significantly potentiated after ATOX1 silencing. The copper chelator TPEN (N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine) produced a form of cell toxicity that was reversed by the addition of Cu2 +. ATOX1 silencing increased Caco-2 cell sensitivity to TPEN toxicity. Our results suggest the possibility of a therapy with copper-chelating or ionophore drugs in subtypes of tumors showing specific alterations in ATOX1 expression. © 2016 Elsevier Inc. All rights reserved.
Nessun risultato.
Nessun risultato.

Contiene: [X]      Estesa     Autori: [X]  Tipo di lavoro: [X]
Data iniziale: Data finale: [X]      Sede: Affiliazione IBB     
[Pulisci modulo]

Lanza V, Milardi D, Di Natale G, Pappalardo G
* Repurposing of Copper(II)-chelating Drugs for the Treatment of Neurodegenerative Diseases (58 visite)
Current Medicinal Chemistry (ISSN: 1875-533xelectronic, 0929-8673linking), 2018 Feb 12; 25(4): 525-539.
Impact Factor: 3.249
Dettagli    Esporta in BibTeX    Esporta in EndNote

Parenti G, Fecarotta S, la Marca G, Rossi B, Ascione S, Donati MA, Morandi LO, Ravaglia S, Pichiecchio A, Ombrone D, Sacchini M, Pasanisi MB, De Filippi P, Danesino C, Della Casa R, Romano A, Mollica C, Rosa M, Agovino T, Nusco E, Porto C, Andria G
* A Chaperone Enhances Blood Alpha-Glucosidase Activity In Pompe Disease Patients Treated With Enzyme Replacement Therapy (79 visite)
Molecular Therapy, 2014 Nov; 22(11): 2004-2012.
Impact Factor: 6.425
Dettagli    Esporta in BibTeX    Esporta in EndNote

Andreotti G, Citro V, Correra A, Cubellis MV
* A thermodynamic assay to test pharmacological chaperones for Fabry disease (115 visite)
Biochim Biophys Acta (ISSN: 0304-4165, 0006-3002, 0925-4439), 2014 Mar; 1840(3): 1214-1224.
Impact Factor: 4.381
Dettagli    Esporta in BibTeX    Esporta in EndNote

Federico A, Zappavigna S,  Romano M, Grieco P, Luce A, Marra M, Gravina AG, Stiuso P, D'Armiento FP, Vitale G, Tuccillo C, Novellino E, Loguercio C, Caraglia M
* Urotensin-II receptor is over-expressed in colon cancer cell lines and in colon carcinoma in humans (132 visite)
European Journal Of Clinical Investigation (ISSN: 0014-2972, 1365-2362), 2014 Mar; 44(3): 285-294.
Impact Factor: 2.734
Dettagli    Esporta in BibTeX    Esporta in EndNote

Cammisa M, Correra A, Andreotti G, Cubellis MV
* Fabry_CEP: a tool to identify Fabry mutations responsive to pharmacological chaperones (137 visite)
Orphanet Journal Of Rare Diseases (ISSN: 1750-1172), 2013 Jul 24; 8: 111-111.
Impact Factor: 3.958
Dettagli    Esporta in BibTeX    Esporta in EndNote

Andreotti G, Citro V, De Crescenzo A, Orlando P, Cammisa M, Correra A, Cubellis MV
* Therapy of Fabry disease with pharmacological chaperones: from in silico predictions to in vitro tests (121 visite)
Orphanet Journal Of Rare Diseases (ISSN: 1750-1172), 2011 Oct 17; 6: 66-66.
Impact Factor: 5.074
Dettagli    Esporta in BibTeX    Esporta in EndNote

Andreotti G, Guarracino MR, Cammisa M, Correra A, Cubellis MV
* Prediction of the responsiveness to pharmacological chaperones: lysosomal human alpha-galactosidase, a case of study (115 visite)
Orphanet Journal Of Rare Diseases (ISSN: 1750-1172), 2010 Dec 8; 5: 36-36.
Impact Factor: 5.933
Dettagli    Esporta in BibTeX    Esporta in EndNote

Nastro P, Sodo M, Dodaro CA, Gargiulo  S, Acampa W, Bracale U, Renda A
* Intraoperative radiochromoguided mapping of sentinel lymph node in colon cancer (96 visite)
Tumori (ISSN: 0300-8916, 0300-8916print), 2002 Jul; 88(4): 352-353.
Impact Factor: 0.267
Dettagli    Esporta in BibTeX    Esporta in EndNote

Russo GL, Della Pietra V, Mercurio C, Palumbo R, Iacomino G, Russo M, Tosto M, Zappia V
Protective effects of butyric acid in colon cancer (53 visite)
Adv Exp Med Biol (ISSN: 0065-2598print, 0065-2598linking), 1999; 472: 131-147.
Impact Factor: 0.446
Dettagli    Esporta in BibTeX    Esporta in EndNote

Salvatore M, Lastoria S, Del Vecchio S, Mansi L
Detection of colon cancer with radiolabelled monoclonal antibodies (104 visite)
Nuclear Medicine And Biology (ISSN: 0883-2897, 0969-8051), 1989; 16(2): 103-104.
Impact Factor: 1.042
Dettagli    Esporta in BibTeX    Esporta in EndNote

10 Records (7 escludendo Abstract e Conferenze).
Impact factor totale: 33.509 (18.544 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 38.752 (21.444 escludendo Abstract e Conferenze).

    Esporta in BibTeX    Esporta in EndNote

Ultima modifica di Gennaro Angrisano in data Wednesday 11 May 2016, 18:27:29
435 visite. Ultima visita in data Monday 10 December 2018, 13:45:04

Webmaster and developer: Marco Comerci
Per problemi e suggerimenti: adminibb.cnr.it
Ultimo aggiornamento: Tuesday 11 December 2018, 12:37:42