Oxidative Stress Mediates the Antiproliferative Effects of Nelfinavir in Breast Cancer Cells(111 visite) Soprano M, Sorriento D, Rusciano MR, Maione AS, Limite G, Forestieri P, D'Angelo D, D'Alessio M, Campiglia P, Formisano P, Iaccarino G, Bianco R, Illario M
Plosone (ISSN: 1932-6203, 1932-6203electronic), 2016 Jun 9; 11(6): e0155970-e0155970.
Tipo di articolo: Journal Article,
Impact factor: 2.806, Impact factor a 5 anni: 3.394
*** IBB - CNR *** Department of Translational Medical Science, University of Naples Federico II, Naples, Italy., Institute of Biostructure and Bioimaging (IBB) of the Italian National Research Council (CNR), Naples, Italy., Department of Clinical Medicine and Surgery, Breast Unit, University of Naples Federico II, Naples, Italy., Department of Emergency and Reception, Plastic Surgery Operative Unit, Hospital Center A. Cardarelli, Naples, Italy., Department of Pharmacy, University of Salerno, Salerno, Italy., Department of Medicine and Surgery, University of Salerno, Salerno, Italy., Department of Clinical Medicine and Surgery, Oncology Division, University of Naples Federico II, Naples, Italy.,
The discovery of the anti-proliferative activity of nelfinavir in HIV-free models has encouraged its investigation as anticancer drug. Although the molecular mechanism by which nelfinavir exerts antitumor activity is still unknown, its effects have been related to Akt inhibition. Here we tested the effects of nelfinavir on cell proliferation, viability and death in two human breast cancer cell lines and in human normal primary breast cells. To identify the mechanism of action of nelfinavir in breast cancer, we evaluated the involvement of the Akt pathway as well as the effects of nelfinavir on reactive oxygen species (ROS) production and ROS-related enzymes activities. Nelfinavir reduced breast cancer cell viability by inducing apoptosis and necrosis, without affecting primary normal breast cells. The antitumor activity of nelfinavir was related to alterations of the cell redox state, coupled with an increase of intracellular ROS production limited to cancer cells. Nelfinavir treated tumor cells also displayed a downregulation of the Akt pathway due to disruption of the Akt-HSP90 complex, and subsequent degradation of Akt. These effects resulted to be ROS dependent, suggesting that ROS production is the primary step of nelfinavir anticancer activity. The analysis of ROS-producers and ROS-detoxifying enzymes revealed that nelfinavir-mediated ROS production was strictly linked to flavoenzymes activation. We demonstrated that ROS enhancement represents the main molecular mechanism required to induce cell death by nelfinavir in breast cancer cells, thus supporting the development of new and more potent oxidizing molecules for breast cancer therapy.
Petraglia F, Singh AA, Carafa V, Nebbioso A, Conte M, Scisciola L, Valente S, Baldi A, Mandoli A, Petrizzi VB, Ingenito C, De Falco S, Cicatiello V, Apicella I, Janssen-megens EM, Kim B, Yi G, Logie C, Heath S, Ruvo M, Wierenga ATJ, Flicek P, Yaspo ML, Della Valle V, Bernard O, Tomassi S, Novellino E, Feoli A, Sbardella G, Gut I, Vellenga E, Stunnenberg HG, Mai A, Martens JHA, Altucci L * Combined HAT/EZH2 modulation leads to cancer-selective cell death(78 visite) Oncotarget (ISSN: 1949-2553electronic, 1949-2553linking), 2018 May 22; 9(39): 25630-25646. Impact Factor:5.008 DettagliEsporta in BibTeXEsporta in EndNote
86 Records (78 escludendo Abstract e Conferenze). Impact factor totale: 320.969 (284.71 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 323.474 (286.352 escludendo Abstract e Conferenze).