Home Research Locations About us Organization Staff Contacts Education Login Documents IT
Projects highlights


ISTAPCA (836 views)



Fondazione Veronesi

Fondo Europeo Pesca (248 views)

 INMiND (912 views)

 PRIN (1016 views)

 eHealthNet (839 views)

  Ponrec (794 views)

MFAG Grant (862 views)


 |<  < >  >| 

    Export to BibTeX    Export to EndNote

Effect of C5-Methylation of Cytosine on the UV-Induced Reactivity of Duplex DNA: Conformational and Electronic Factors (26 views)

Banyasz A, Esposito L, Douki T, Perron M, Lepori C, Improta R, Markovitsi D

J Phys Chem B (ISSN: 1520-5207electronic, 1520-5207linking, 1520-6106), 2016 May 12; 120(18): 4232-4242.

C5-methylation of cytosines is strongly correlated with UV-induced mutations detected in skin cancers. Mutational hot-spots appearing at TCG sites are due to the formation of pyrimidine cyclobutane dimers (CPDs). The present study, performed for the model DNA duplex (TCGTA)3.(TACGA)3 and the constitutive single strands, examines the factors underlying the effect of C5-methylation on pyrimidine dimerization at TCG sites. This effect is quantified for the first time by quantum yields varphi. They were determined following irradiation at 255, 267, and 282 nm and subsequent photoproduct analysis using HPLC coupled to mass spectrometry. C5-methylation leads to an increase of the CPD quantum yield up to 80% with concomitant decrease of that of pyrimidine(6-4) pyrimidone adducts (64PPs) by at least a factor of 3. The obtained varphi values cannot be explained only by the change of the cytosine absorption spectrum upon C5-methylation. The conformational and electronic factors that may affect the dimerization reaction are discussed in light of results obtained by fluorescence spectroscopy, molecular dynamics simulations, and quantum mechanical calculations. Thus, it appears that the presence of an extra methyl on cytosine affects the sugar puckering, thereby enhancing conformations of the TC step that are prone to CPD formation but less favorable to 64PPs. In addition, C5-methylation diminishes the amplitude of conformational motions in duplexes; in the resulting stiffer structure, pipi* excitations may be transferred from initially populated exciton states to reactive pyrimidines giving rise to CPDs.

Affiliations ▼
*** IBB - CNR Affiliation

LIDYL, CEA, CNRS, Universite Paris Saclay, F-91191 Gif-sur-Yvette, France., Istituto Biostrutture e Bioimmagini - CNR , 80134 Napoli, Italy., Universite Grenoble Alpes, INAC, LCIB, LAN & CEA, INAC, SCIB, LANF-38000 Grenoble, France.,

Details ▼
Impact factor: 3.377, 5-year impact factor: 3.527

Paper type: Journal Article,

Keywords: Not available.

Url: Not available.

References ▼
Not available.

Related activities ▼
Relaxometry based MRI brain segmentation (2843 views)
Novel Partial Volume Effect Correction algorithm development (737 views)
Assessment of the cardiac risk and its temporal variation (2734 views)
Partial Volume Effect Correction software maintenance (135 views)
Carbonic Anhydrase family as target for rational drug design (302 views)
Modeling radiation induced normal tissue effects (220 views)
Animal Care, interventional procedures, anesthesia and analgesia of murine models in preclinical imaging (256 views)
High-resolution positron emission tomography of the ischaemic mouse heart (2677 views)
Protein aggregation and cytotoxicity: molecular, in vitro and in vivo biological studies (221 views)
Metals and small molecules in the regulation of the Ubiquitin Proteasome Pathway (223 views)
Targeted Drug Delivery by integrin avb3 antagonist (190 views)
Translational Molecular Imaging in Cancer (261 views)
Peptides promoting reverse cholesterol transport (190 views)
Molecular imaging for personalized therapy in oncology (163 views)
Structure-based design of artificial activators that reactivate gene expression in vivo (270 views)
Portable devices for radioguided surgery (335 views)
New techniques for advanced detection (257 views)
Integration of the SPECT modules to innovative Quad-modality Molecular Imaging System for Small Animal (PET / SPECT / CT/ Fluorescence device) (4766 views)

Related papers ▼
Effects of a novel Nodal-targeting monoclonal antibody in melanoma (restricted access) (60 views)
Regulating levels of the neuromodulator D-serine in human brain: structural insight into pLG72 and D-amino acid oxidase interaction (public domain) (49 views)
Vascular Disease in Patients with Multiple Sclerosis: A Review (public domain) (41 views)

Related bibliography ▼

Content: [X]

Authors: [X]
AND Paper type: [X]

AND Start date: End date: [X]   AND Site:    AND  IBB affiliation

[Clear form]

<=2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017   ALL YEARS

Sort by: date    title    journal    authors      impact factor

* UV-induced damage to DNA: effect of cytosine methylation on pyrimidine dimerization (6 views)
Martinez-fernandez L, Banyasz A, Esposito L, Markovitsi D, Improta R
Signal Transduction And Targeted Therapy, 2017 Jun; N/D: N/D-N/D.

View    Export to BibTeX    Export to EndNote    Upload PDF

1 Records (1 excluding Abstracts and Conferences).
Total impact factor: 0 (0 excluding Abstracts and Conferences).
Total 5-year impact factor: 0 (0 excluding Abstracts and Conferences).

Your bibliography query: (([btitle, keywords, abstract] EFFECT AND [btitle, keywords, abstract] METHYLATION AND [btitle, keywords, abstract] CYTOSINE AND [btitle, keywords, abstract] INDUCED AND [btitle, keywords, abstract] REACTIVITY AND [btitle, keywords, abstract] DUPLEX AND [btitle, keywords, abstract] CONFORMATIONAL)) AND NOT [id] = 53281

Last modified by Gennaro Angrisano on Friday 01 July 2016, 11:59:05
26 views. Last viewed on Thursday 14 September 2017, 13:47:40

Webmaster and developer: Marco Comerci
For problems and suggestions: adminibb.cnr.it
Last updated: Wednesday 20 September 2017, 10:43:29