Destabilisation, aggregation, toxicity and cytosolic mislocalisation of nucleophosmin regions associated with acute myeloid leukemia (380 views visite)
Oncotarget (ISSN: 1949-2553, 1949-2553electronic, 1949-2553linking), 2016 Sep 13; 7(37): 59129-59143.
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Paper type Tipo di articolo: Journal Article
Impact factor: 5.168, 5-year impact factor Impact factor a 5 anni: 5.312
Url: Not available. Non disponibile.
Keywords Parole chiave: Aml, Cd Spectroscopy, Aggregation Phenomena, Helical Peptides, Apoptosis , Cell Line, Tumor , Cell Nucleus Metabolism , Cytosol Metabolism , Humans , Leukemia, Myeloid, Acute Metabolism , Nuclear Proteins Metabolism , Peptide Fragments , Protein Aggregation, Pathological , Protein Conformation , Protein Stability , Protein Transport
Affiliations Affiliazioni:
*** IBB - CNR ***
Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi-University of Naples "Federico II", DFM-Scarl, 80134, Naples, Italy., Permanent address: Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, 80125 Napoli, Italy., Institute of Biostructures and Bioimaging - CNR, 80134, Naples, Italy., Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, 50134, Florence, Italy., Laboratory of Molecular Biology and DNA repair, Department of Medical and Biological Sciences, University of Udine, 33100, Udine, Italy., Permanent address: Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, Nordbyhagen, 1474, Norway.,
Permanent address: Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, 80125, Napoli, Italy.
Institute of Biostructures and Bioimaging - CNR, 80134, Naples, Italy.
Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, 50134, Florence, Italy.
Laboratory of Molecular Biology and DNA repair, Department of Medical and Biological Sciences, University of Udine, 33100, Udine, Italy.
References Riferimenti:
Not available. Non disponibili.
Oncotarget (ISSN: 1949-2553, 1949-2553electronic, 1949-2553linking), 2016 Sep 13; 7(37): 59129-59143.
Export to Esporta in BibTeX Export to Esporta in EndNote
Paper type Tipo di articolo: Journal Article
Impact factor: 5.168, 5-year impact factor Impact factor a 5 anni: 5.312
Url: Not available. Non disponibile.
Keywords Parole chiave: Aml, Cd Spectroscopy, Aggregation Phenomena, Helical Peptides, Apoptosis , Cell Line, Tumor , Cell Nucleus Metabolism , Cytosol Metabolism , Humans , Leukemia, Myeloid, Acute Metabolism , Nuclear Proteins Metabolism , Peptide Fragments , Protein Aggregation, Pathological , Protein Conformation , Protein Stability , Protein Transport
Affiliations Affiliazioni:
*** IBB - CNR ***
Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi-University of Naples "Federico II", DFM-Scarl, 80134, Naples, Italy., Permanent address: Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, 80125 Napoli, Italy., Institute of Biostructures and Bioimaging - CNR, 80134, Naples, Italy., Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, 50134, Florence, Italy., Laboratory of Molecular Biology and DNA repair, Department of Medical and Biological Sciences, University of Udine, 33100, Udine, Italy., Permanent address: Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, Nordbyhagen, 1474, Norway.,
Permanent address: Center for Advanced Biomaterials for Health Care@CRIB Istituto Italiano di Tecnologia, 80125, Napoli, Italy.
Institute of Biostructures and Bioimaging - CNR, 80134, Naples, Italy.
Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, 50134, Florence, Italy.
Laboratory of Molecular Biology and DNA repair, Department of Medical and Biological Sciences, University of Udine, 33100, Udine, Italy.
References Riferimenti:
Not available. Non disponibili.
Destabilisation, aggregation, toxicity and cytosolic mislocalisation of nucleophosmin regions associated with acute myeloid leukemia
Nucleophosmin (NPM1) is a multifunctional protein that is implicated in the pathogenesis of several human malignancies. To gain insight into the role of isolated fragments of NPM1 in its biological activities, we dissected the C-terminal domain (CTD) into its helical fragments. Here we focus the attention on the third helix of the NPM1-CTD in its wild-type (H3 wt) and AML-mutated (H3 mutA and H3 mutE) sequences. Conformational studies, by means of CD and NMR spectroscopies, showed that the H3 wt peptide was partially endowed with an alpha-helical structure, but the AML-sequences exhibited a lower content of this conformation, particularly the H3 mutA peptide. Thioflavin T assays showed that the H3 mutE and the H3 mutA peptides displayed a significant aggregation propensity that was confirmed by CD and DLS assays. In addition, we found that the H3 mutE and H3 mutA peptides, unlike the H3 wt, were moderately and highly toxic, respectively, when exposed to human neuroblastoma cells. Cellular localization experiments confirmed that the mutated sequences hamper their nucleolar accumulation, and more importantly, that the helical conformation of the H3 region is crucial for such a localization.
Nucleophosmin (NPM1) is a multifunctional protein that is implicated in the pathogenesis of several human malignancies. To gain insight into the role of isolated fragments of NPM1 in its biological activities, we dissected the C-terminal domain (CTD) into its helical fragments. Here we focus the attention on the third helix of the NPM1-CTD in its wild-type (H3 wt) and AML-mutated (H3 mutA and H3 mutE) sequences. Conformational studies, by means of CD and NMR spectroscopies, showed that the H3 wt peptide was partially endowed with an alpha-helical structure, but the AML-sequences exhibited a lower content of this conformation, particularly the H3 mutA peptide. Thioflavin T assays showed that the H3 mutE and the H3 mutA peptides displayed a significant aggregation propensity that was confirmed by CD and DLS assays. In addition, we found that the H3 mutE and H3 mutA peptides, unlike the H3 wt, were moderately and highly toxic, respectively, when exposed to human neuroblastoma cells. Cellular localization experiments confirmed that the mutated sequences hamper their nucleolar accumulation, and more importantly, that the helical conformation of the H3 region is crucial for such a localization.
Destabilisation, aggregation, toxicity and cytosolic mislocalisation of nucleophosmin regions associated with acute myeloid leukemia
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Destabilisation, aggregation, toxicity and cytosolic mislocalisation of nucleophosmin regions associated with acute myeloid leukemia
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Impact factor totale: 53.437 (49.249 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 48.998 (41.835 escludendo Abstract e Conferenze).
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Riccardi C, Capasso D, Coppola A, Platella C, Montesarchio D, Di Gaetano S, Roviello GN, Musumeci D
* Synthesis, Antiproliferative Activity, and DNA Binding Studies of Nucleoamino Acid-Containing Pt(II) Complexes (122 visite)
Pharmaceuticals (ISSN: 1424-8247linking), 2020 Sep 30; 13(10): 284-284.
Impact Factor: 4.421
Dettagli Esporta in BibTeX Esporta in EndNote
D’errico S, Falanga AP, Capasso D, Di Gaetano S, Marzano M, Terracciano M, Roviello GN, Piccialli G, Oliviero G, Borbone N
* Probing the DNA Reactivity and the Anticancer Properties of a Novel Tubercidin-Pt(II) Complex (114 visite)
Pharmaceutics (ISSN: 1999-4923linking), 2020 Jul 4; 12(7): 627-627.
Impact Factor: 4.421
Dettagli Esporta in BibTeX Esporta in EndNote
Riccardi C, Capasso D, Rozza GM, Platella C, Montesarchio D, Di Gaetano S, Marzo T, Pratesi A, Messori L, Roviello GN, Musumeci D
* Synthesis, DNA binding studies, and antiproliferative activity of novel Pt(II)- complexes with an L-alanyl-based ligand (232 visite)
J Inorg Biochem (ISSN: 0162-0134linking), 2020 Feb; 203: 110868-110868.
Impact Factor: 3.224
Dettagli Esporta in BibTeX Esporta in EndNote
Mercurio FA, Marasco D, Di Natale C, Pirone L, Costantini S, Pedone EM, Leone M
* Targeting EphA2-Sam and Its Interactome: Design and Evaluation of Helical Peptides Enriched in Charged Residues (385 visite)
Chembiochem (ISSN: 1439-4227, 1439-7633, 1439-4227linking), 2016 Nov 17; 17(22): 2179-2188.
Impact Factor: 2.847
Dettagli Esporta in BibTeX Esporta in EndNote
Roviello GN, Vicidomini C, Di Gaetano S, Capasso D, Musumeci D, Roviello V
* Solid phase synthesis and RNA-binding activity of an arginine-containing nucleopeptide (359 visite)
Rsc Adv (ISSN: 2046-2069), 2016; 6(17): 14140-14148.
Impact Factor: 3.108
Dettagli Esporta in BibTeX Esporta in EndNote
Galdiero S, Falanga A, Berisio R, Grieco P, Morelli G, Galdiero M
* Antimicrobial peptides as an opportunity against bacterial diseases (651 visite)
Curr Med Chem (ISSN: 0929-8673, 1875-533x, 1875-533xelectronic), 2015 Mar 11; 22(14): 1665-1677.
Impact Factor: 3.455
Dettagli Esporta in BibTeX Esporta in EndNote
Correale S, Esposito C, Pirone L, Vitagliano L, Di Gaetano S, Pedone E
* A biophysical characterization of the folded domains of KCTD12: insights into interaction with the GABA(B2) receptor (301 visite)
Journal Of Molecular Recognition (ISSN: 0952-3499, 0952-2349), 2013 Oct; 26(10): 488-495.
Impact Factor: 2.337
Dettagli Esporta in BibTeX Esporta in EndNote
Stelitano V, Brandt A, Fernicola S, Franceschini S, Giardina G, Pica A, Rinaldo S, Sica F, Cutruzzola F
* Probing the activity of diguanylate cyclases and c-di-GMP phosphodiesterases in real-time by CD spectroscopy (442 visite)
Nucleic Acids Res (ISSN: 0305-1048, 0305-5104, 1362-4962), 2013 Apr; 41(7): e79-e79.
Impact Factor: 4.188
Dettagli Esporta in BibTeX Esporta in EndNote
Vitagliano L, Fiume G, Scognamiglio PL, Doti N, Cannavo R, Puca A, Pedone C, Scala G, Quinto I, Marasco D
* Structural and functional insights into IkappaB-alpha/HIV-1 Tat interaction (531 visite)
Biochimie (ISSN: 0300-9084, 1638-6183, 1638-6183electronic), 2011 Sep; 93(9): 1592-1600.
Impact Factor: 3.022
Dettagli Esporta in BibTeX Esporta in EndNote
Ronga L, Langella E, Palladino P, Marasco D, Tizzano B, Saviano M, Pedone C, Improta R, Ruvo M
* Does Tetracycline Bind Helix 2 Of Prion? An Integrated Spectroscopical And Computational Study Of The Interaction Between The Antibiotic And Alpha Helix 2 Human Prion Protein Fragments (435 visite)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2007 Feb 15; 66(3): 707-715.
Impact Factor: 3.354
Dettagli Esporta in BibTeX Esporta in EndNote
Marasco D, Saporito A, Ponticelli S, Chambery A, De Falco S, Pedone C, Minchiotti G, Ruvo M
* Chemical synthesis of mouse Cripto CFC variants (343 visite)
Proteins (ISSN: 0887-3585, 1097-0134, 1097-0134electronic), 2006 Sep 15; 64(3): 779-788.
Impact Factor: 3.73
Dettagli Esporta in BibTeX Esporta in EndNote
D'Andrea LD, Iaccarino G, Fattorusso R, Sorriento D, Carannante C, Capasso D, Trimarco B, Pedone C
* Targeting angiogenesis: Structural characterization and biological properties of a de novo engineered VEGF mimicking peptide (483 visite)
P Natl Acad Sci Usa (ISSN: 0027-8424, 1091-6490, 0027-8424print), 2005 Oct 4; 102(40): 14215-14220.
Impact Factor: 10.231
Dettagli Esporta in BibTeX Esporta in EndNote
Zell R, Sidigi K, Bucci E, Stelzner A, Goerlach M
* Determinants of the recognition of enteroviral cloverleaf RNA by coxsackievirus B3 proteinase 3C (378 visite)
Rna (ISSN: 1355-8382), 2002; 8(2): 188-201.
Impact Factor: 5.099
Dettagli Esporta in BibTeX Esporta in EndNote
13 Records (12 escludendo Abstract e Conferenze).
Impact factor totale: 53.437 (49.249 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 48.998 (41.835 escludendo Abstract e Conferenze).
Last modified by Ultima modifica di Marco Comerci on in data Wednesday 02 December 2020, 18:09:25
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