Impact factor: 3.123, Impact factor a 5 anni: 2.555
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Parole chiave: Antibody Binding, Conformational Change, Molecular Dynamics, Protein Flexibility, Viral Proteins,
*** IBB - CNR *** a Institute of Biostructures and Bioimaging, C.N.R. , Naples I-80134 , Italy., b Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche , Seconda Universita di Napoli , Caserta 81100 , Italy.,
Hepatitis C Virus (HCV) is one of the most persistent human viruses. Although effective therapeutic approaches have been recently discovered, their use is limited by the elevated costs. Therefore, the development of alternative/complementary strategies is an urgent need. The E2 glycoprotein, the most immunogenic HCV protein, and its variants represent natural candidates to achieve this goal. Here we report an extensive molecular dynamics (MD) analysis of the intrinsic properties of E2. Our data provide interesting clues on the global and local intrinsic dynamic features of the protein. Present MD data clearly indicate that E2 combines a flexible structure with a network of covalent bonds. Moreover, the analysis of the two most important antigenic regions of the protein provides some interesting insights into their intrinsic structural and dynamic properties. Our data indicate that a fluctuating beta-hairpin represents a populated state by the region E2412-423. Interestingly, the analysis of the epitope E2427-446 conformation, that undergoes a remarkable rearrangement in the simulation, has significant similarities with the structure that the E2430-442 fragment adopts in complex with a neutralizing antibody. Present data also suggest that the strict conservation of Gly436 in E2 protein of different HCV genotypes is likely dictated by structural restraints. Moreover, the analysis of the E2412-423 flexibility provides insights into the mechanisms that some antibodies adopt to anchor Trp437 that is fully buried in E2. Finally, the present investigation suggests that MD simulations should systematically complement crystallographic studies on flexible proteins that are studied in combination with antibodies.
181 Records (176 escludendo Abstract e Conferenze). Impact factor totale: 700.641 (678.558 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 740.951 (718.715 escludendo Abstract e Conferenze).