Long range Trp-Trp interaction initiates the folding pathway of a pro-angiogenic beta-hairpin peptide(309 views visite) Diana D, De Rosa L, Palmieri M, Russomanno A, Russo L, La Rosa C, Milardi D, Colombo G, D'Andrea LD, Fattorusso R
Keywords Parole chiave: Amino Acid Sequence, Calorimetry, Differential Scanning, Hydrogen Bonding, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Dynamics Simulation, Mutagenesis, Peptides, Chemistry, Genetics, Metabolism, Protein Folding, Protein Structure, Secondary, Protein Unfolding, Thermodynamics, Transition Temperature, Tryptophan,
Affiliations Affiliazioni: *** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, C.N.R., via Mezzocannone 16, 80134, Napoli (Italy)., Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Universita degli Studi Napoli, via Vivaldi 43, 81100, Caserta (Italy)., Dipartimento di Scienze Chimiche, Universita degli Studi di Catania, viale A. Doria 6, 95125, Catania (Italy)., Istituto di Biostrutture e Bioimmagini, C.N.R., Via Gaifami 18, 95126, Catania (Italy)., Istituto di Chimica del Riconoscimento Molecolare, C.N.R., via Bianco 9, 20131, Milano (Italy).,
References Riferimenti: Not available. Non disponibili.
Long range Trp-Trp interaction initiates the folding pathway of a pro-angiogenic beta-hairpin peptide
HPLW, a designed VEGF (Vascular Endothelium Growth Factor) receptor-binding peptide, assumes a well folded beta-hairpin conformation in water and is able to induce angiogenesis in vivo. In this study, we investigated at atomic resolution the thermal folding/unfolding pathway of HPLW by means of an original multi-technique approach combining DSC, NMR, MD and mutagenesis analyses. In particular, careful NMR investigation of the single proton melting temperatures together with DSC analysis accurately delineate the peptide folding mechanism, which is corroborated by computational folding/unfolding simulations. The HPLW folding process consists of two main events, which are successive but do not superimpose. The first folding step initiates at 320 K upon the hydrophobic collapse of the Trp5 and Trp13 side-chains which stabilizes the concurrent beta-turn formation, whose COi-HNi + 3 hydrogen bond (Asp10 --> Arg7) appears particularly stable. At 316 K, once the beta-turn is completely formed, the two beta-strands pair, very likely starting by Trp5 and Trp13, which thus play a key role also in the final step of the beta-hairpin folding. Overall, here we describe a multi-state hierarchical folding pathway of a highly structured beta-hairpin, which can be classified as a broken-zipper mechanism.
Long range Trp-Trp interaction initiates the folding pathway of a pro-angiogenic beta-hairpin peptide
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ * Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer(211 visite) N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756. Impact Factor:59.558 DettagliEsporta in BibTeXEsporta in EndNote
Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(371 visite) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 DettagliEsporta in BibTeXEsporta in EndNote
693 Records (682 escludendo Abstract e Conferenze). Impact factor totale: 2654.265 (2619.376 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 2713.398 (2673.482 escludendo Abstract e Conferenze).
Last modified by Ultima modifica di Donatella Diana on in data Sunday 12 July 2020, 13:14:48 309 views visite. Last view on Ultima visita in data Friday 22 January 2021, 23:57:11