Ubiquitin Associates with the N-Terminal Domain of Nerve Growth Factor: The Role of Copper(II) Ions(97 visite)(PDF privato89 visite) Lanza V, Travaglia A, Malgieri G, Fattorusso R, GrassoG, Di Natale G, Zito V, Arena G, Milardi D, Rizzarelli E
CHEM-EUR J (ISSN: 0947-6539), 2016 Dec 05; 22(49): 17767-17775.
Tipo di articolo: Journal Article,
Impact factor: 5.317, Impact factor a 5 anni: 5.184
*** IBB - CNR *** Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche, Via P. Gaifami18, 95126, Catania, Italy., Center for Neural Science, New York University, 4 Washington Pl, New York, NY, 10003, USA., Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Universita di Napoli, Via Vivaldi 43, 81100, Caserta, Italy., Dipartimento di Scienze Chimiche, Universita degli Studi di Catania, V.le A.Doria 6, 95125, Catania, Italy.,
Many biochemical pathways involving nerve growth factor (NGF), a neurotrophin with copper(II) binding abilities, are regulated by the ubiquitin (Ub) proteasome system. However, whether NGF binds Ub and the role played by copper(II) ions in modulating their interactions have not yet been investigated. Herein NMR spectroscopy, circular dichroism, ESI-MS, and titration calorimetry are employed to characterize the interactions of NGF with Ub. NGF1-14 , which is a short model peptide encompassing the first 14 N-terminal residues of NGF, binds the copper-binding regions of Ub (KD =8.6 10-5 m). Moreover, the peptide undergoes a random coil-polyproline type II helix structural conversion upon binding to Ub. Notably, copper(II) ions inhibit NGF1-14 /Ub interactions. Further experiments performed with the full-length NGF confirmed the existence of a copper(II)-dependent association between Ub and NGF and indicated that the N-terminal domain of NGF was a valuable paradigm that recapitulated many traits of the full-length protein.
22 Records (20 escludendo Abstract e Conferenze). Impact factor totale: 67.17 (62.282 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 71.098 (65.614 escludendo Abstract e Conferenze).