Characterization of linear mimetic peptides of Interleukin-22 from dissection of protein interfaces(241 visite) La Manna S, Scognamiglio PL, Di Natale C, Leone M, Mercurio FA, Malfitano AM, Cianfarani F, Madonna S, Caravella S, Albanesi C, Novellino E, Marasco D
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2017 May 04; N/D: N/D-N/D.
Tipo di articolo: Journal Article,
Impact factor: 3.188, Impact factor a 5 anni: 3.058
Url: Non disponibile.
Parole chiave: Circular Dichroism, Il-22 Signaling, Interface Protein Regions,
*** IBB - CNR *** Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples "Federico II", 80134, Naples, Italy., Institute of Biostructures and Bioimaging, CNR, 80134, Naples, Italy., Laboratory of Cellular and Molecular Biology, Fondazione "Luigi Maria Monti", Istituto Dermopatico dell'Immacolata (IDI), IRCCS, Via Monti di Creta, 104, 00167, Rome, Italy., Laboratory of Experimental Immunology, Fondazione "Luigi Maria Monti", Istituto Dermopatico dell'Immacolata (IDI), IRCCS, Via Monti di Creta, 104, 00167, Rome, Italy., Department of Pharmacy, CIRPEB: Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples "Federico II", 80134, Naples, Italy. Electronic address: firstname.lastname@example.org.,
Interleukin-22 (IL-22) belongs to the family of IL-10 cytokines and is involved in a wide number of human diseases, including inflammatory disorders and cancer pathology. The ligand-receptor complex IL-22/IL-22R plays a key role in several pathways especially in the regulation and resolution of immune responses. The identification of novel compounds able to modulate IL-22/IL-22R complex could open the route to new therapeutic strategies in multiple human diseases. In this study, we designed and characterized IL-22 derived peptides at protein interface regions: several sequences revealed able to interfere with the protein complex with IC50 in the micromolar range as evaluated through Surface Plasmon Resonance (SPR) experiments. Their conformational characterization was carried out through Circular Dichroism (CD) and Nuclear Magnetic Resonance (NMR) spectroscopies, shedding new light into the features of IL-22 fragments and on structural determinants of IL-22/IL-22R1 recognition. Finally, several peptides were tested on human keratinocyte cultures for evaluating their ability to mimic the activation of molecular pathways downstream to IL-22R in response to IL-22 binding.
237 Records (232 escludendo Abstract e Conferenze). Impact factor totale: 882.258 (859.965 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 914.837 (891.211 escludendo Abstract e Conferenze).