Detection of oligonucleotides by PNA-peptide conjugates recognizing the biarsenical fluorescein complex FlAsH-EDT2(155 visite) Piras L, Avitabile C, D'Andrea LD, Saviano M, Romanelli A
Biochem Bioph Res Co (ISSN: 0006-291x), 2017 Sep 15; N/D: N/D-N/D.
Tipo di articolo: Journal Article,
Impact factor: 2.559, Impact factor a 5 anni: 2.455
Url: Non disponibile.
Parole chiave: Duplex, Flash-Edt(2), Hybridization, Mycn, Peptide Nucleic Acids, Mir 17-5p,
*** IBB - CNR *** Institute of Crystallography (IC), CNR, Via Amendola 122/O, 70126, Bari, Italy., Institute of Biostructures and Bioimaging (IBB), CNR, via Mezzocannone 16, 80134, Napoli, Italy., Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, 80134, Napoli, Italy. Electronic address: email@example.com.,
We report the application of the arsenical complex FlAsH-EDT2 for the identification of oligonucleotide sequences. We designed PNA sequences conjugated to either a tetracysteine motif and to split tetracysteine sequences, that are recognized by FlAsH. The effect of conjugation of the PNA to the tetracysteine peptide and RNA hybridization on the fluorescence of the arsenical complex has been investigated. The reconstitution of the tetracysteine motif, starting from 15-mer PNAs conjugated to split tetracysteine sequences and hybridized to a complementary oligonucleotide was also explored.
39 Records (36 escludendo Abstract e Conferenze). Impact factor totale: 113.028 (109.15 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 113.917 (107.045 escludendo Abstract e Conferenze).