Institute of Biostructures and Bioimages of National Council of Research, Naples, Italy; CEINGE scarl, Naples, Italy
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Noninvasive Ovarian Imaging in the Murine Models Using Ultrasound Biomicroscopy
The laboratory mouse is a well-accepted model for reproductive biology studies and findings made in mice have been commonly translated to humans. C57BL/6J and BALB/c strains provide a robust experimental platform for polycystic ovary syndrome and ovarian cancer research in women. Serial assessment of ovarian morphometry and follicle dynamics may be performed non-invasively and reliably in mice by Ultrasound Biomicroscopy (UBM), with a near microscopic resolution. Our pilot study focused on the utility of UBM in murine models to measure size of ovaries and to count ovarian follicles over time. Three C57Bl/6J and two BALB/c adult mice (>8 weeks of age) were housed in standard conditions, with free access to food and water. Spontaneous estrous cycle was longitudinally evaluated by transcutaneous UBM (Vevo 770, VisualSonics, Toronto, Canada; center frequency of 40 MHz; focal depth of 6 mm, spatial resolution of 30 um) on a time period of 8-15 consecutive days. Mice were anesthetized with 1.5% isoflurane in oxygen and fixed in sternal recumbency on a heated stage. Trichotomy was performed over the thoracolumbar area, and acoustic gel was applied to the skin. Images of both ovaries were obtained in sagittal and axial scans, and processed offline using ImageJ software. Ovarian diameters, area, volume and follicles identification were obtained. To imaging results comparison, the phase of the estrous cycle was assessed in BALB/c mice by vaginal cytology. Ovaries were consistently localized in all mice, with a 10 minutes scan time. The changes of the follicles pattern and number overtime have wave-like fashion. In conclusion, UBM is a suitable method for measuring and for identifying the patterns in follicle development, ovulation and regression in mice in vivo, and to monitor the potential efficacy of novel therapies in murine models of ovarian pathologies.
Noninvasive Ovarian Imaging in the Murine Models Using Ultrasound Biomicroscopy
4 Records (0 escludendo Abstract e Conferenze). Impact factor totale: 4.412 (0 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 4.171 (0 escludendo Abstract e Conferenze).
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