Institute of Biostructures and Bioimages of National Council of Research, Naples, Italy; CEINGE scarl, Naples, Italy
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Animal Care in a Mouse Model of Amyotrophic Lateral Sclerosis Studied with [18F]DPA-714 Micro-PET/CT
SOD1G93A mice are a transgenic model of Amyotrophic lateral sclerosis (ALS) to investigate in vivo neuroinflammation with innovative translocator protein radioligands and positron emission tomography (PET). The possibility to perform serial studies in the same animal, from the asymptomatic to the advanced stages, is highly desirable. Alterations of nutrition, hydration and of cardiovascular hemodynamics provide challenges for researchers and can adversely affect imaging studies. 10 hemyzigous SOD1G93A mice (aged 71-137 days) were evaluated at different clinical stages (CS, score range: 0-4) by [18F]DPA-714 PET/CT (GE Healthcare eXplore Vista, resolution: 1.8 mm FWHM/200 μm). 5 mice were longitudinally monitored. Weight, body condition (BCS, 0-5) and dehydration were daily monitored. From CS and BCS scored as 2, palatable source of hydration and energy were left on cage floor. Glucose 5% /Ringer lactate or NaCl 0.9% solutions (1-3 ml/day) with multivitaminic supplement were provided parenterally. Mice were housed in dry cage with soft bed, avoiding social isolation. Ocular discharges and ulcerative lesions were cleaned (NaCl 0.9%; iodopovidone). Imaging studies were performed under inhalant anesthesia (isoflurane 2% and oxygen 2 L/min). Radiotracer uptake (SUV) was measured on PET/CT fusion images in the cerebellum, brainstem, spinal cord normalized to those of the frontal cortex using Osirix software. In the single PET/CT group, 3 mice died after imaging study (CS: 1-2; BCS 3; weight gain +2.87g), while 2 mice were euthanized after 6 days (CS: 4; BCS 2; weight loss -3.04g). Mice performed from 2 to 5 imaging sessions, with a mean survival of 6 days after the last PET/CT (CS: 2-4; BCS 2-3; weight loss -1.68g). SUV showed an increased trend overtime in brainstem compared to age-matched controls. These findings underlie the relevant role played by the special care of transgenic murine models in order to improve longitudinal studies and minimize confounding variables. The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/2007-2013) under grant agreement n° HEALTH-F2-2011-278850 (INMiND).
Animal Care in a Mouse Model of Amyotrophic Lateral Sclerosis Studied with [18F]DPA-714 Micro-PET/CT
23 Records (18 escludendo Abstract e Conferenze). Impact factor totale: 107.988 (83.547 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 100.067 (78.254 escludendo Abstract e Conferenze).
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