Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy
CNR Ibb | back to homepage
▲NavBar
Ricerca avanzata▼
Contiene: [X]      Estesa     Autori: [X]  Tipo di lavoro: [X]   Data iniziale: Data finale: [X]      Sede: Affiliazione IBB     
    
[Pulisci modulo]


Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy (198 visite)

Calvanese L, Foca A, Sandomenico A, Foca G, Caporale A, Doti N, Iaccarino E, Leonardi A, D'Auria G, Ruvo M, Falcigno L

Bioorg Med Chem (ISSN: 0968-0896, 1464-3391), 2017 Oct 28; N/D: N/D-N/D.



Tipo di articolo: Journal Article,

Impact factor: 2.881, Impact factor a 5 anni: 2.911

Url: Non disponibile.

Parole chiave: Binding, Group Epitope Mapping, Tgf-Beta,

Affiliazioni:

*** IBB - CNR ***
CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy., Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy., DISTABIF, Universita degli Studi della Campania "Lugi Vanvitelli", via Vivaldi, 43, 80100 Caserta, Italy., Dept. Medicina Molecolare e Biotecnologie Mediche, Universita Federico II di Napoli, Naples, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy; Dept. of Pharmacy, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy. Electronic address: menotti.ruvo@unina.it., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy; Dept. of Pharmacy, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy. Electronic address: lucia.falcigno@unina.it.,


Riferimenti:

Non disponibile.

Nodal is a growth factor expressed during early embryonic development, but reactivated in several advanced-stage cancers. Targeting of Nodal signaling, which occurs via the binding to Cripto-1 co-receptor, results in inhibition of cell aggressiveness and reduced tumor growth. The Nodal binding region to Cripto-1 was identified and targeted with a high affinity monoclonal antibody (3D1). By STD-NMR technique, we investigated the interaction of Nodal fragments with 3D1 with the aim to elucidate at atomic level the interaction surface. Data indicate with high accuracy the antibody-antigen contact atoms and confirm the information previously obtained by immune-enzymatic methods. Main residues contacted by 3D1 are P46, V47, E49 and E50, which belong to the Nodal loop involved in the interaction with the co-receptor.
Nessun risultato.
Nessun risultato.

Ricerca avanzata▼
Contiene: [X]      Estesa     Autori: [X]  Tipo di lavoro: [X]   Data iniziale: Data finale: [X]      Sede: Affiliazione IBB     
    
[Pulisci modulo]
Vai a fine pagina   Versione per copia e incolla facile   Esporta tutto in BibTeX   Esporta tutto in EndNote


Ferrucci V, De Antonellis P, Pennino FP, Asadzadeh F, Virgilio A, Montanaro D, Galeone A, Boffa I, Pisano I, Scognamiglio I, Navas L, Diana D, Pedone E, Gargiulo S, Gramanzini M, Brunetti A, Danielson L, Carotenuto M, Liguori L, Verrico A, Quaglietta L, Errico ME, Del Monaco V, D'Argenio V, Tirone F, Mastronuzzi A, Donofrio V, Giangaspero F, Picard D, Remke M, Garzia L, Daniels C, Delattre O, Swartling FJ, Weiss WA, Salvatore F, Fattorusso R, Chesler L, Taylor MD, Cinalli G, Zollo M
* Metastatic group 3 medulloblastoma is driven by PRUNE1 targeting NME1-TGF-beta-OTX2-SNAIL via PTEN inhibition (109 visite)
Brain (ISSN: 1460-2156electronic, 0006-8950linking), 2018 May 1; 141(5): 1300-1319.
Impact Factor: 9.915
Dettagli    Esporta in BibTeX    Esporta in EndNote

Calvanese L, Sandomenico A, Caporale A, Foca A, Foca G, D'Auria G, Falcigno L, Ruvo M
* Conformational features and binding affinities to Cripto, ALK7 and ALK4 of Nodal synthetic fragments (168 visite)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617linking), 2015 Jan 15; 21(4): 283-293.
Impact Factor: 1.951
Dettagli    Esporta in BibTeX    Esporta in EndNote

Romano S, D'Angelillo A, D'Arrigo P, Staibano S, Greco A, Brunetti A, Scalvenzi M, Bisogni R, Scala I, Romano MF
* FKBP51 increases the tumour-promoter potential of TGF-beta (125 visite)
Clinical And Translational Medicine, 2014 Jan 27; 3(1): 1-1.
Impact Factor: 0
Dettagli    Esporta in BibTeX    Esporta in EndNote



3 Records (2 escludendo Abstract e Conferenze).
Impact factor totale: 11.866 (11.866 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 12.577 (12.577 escludendo Abstract e Conferenze).







    Esporta in BibTeX    Esporta in EndNote