Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy
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Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy (152 visite)

Calvanese L, Foca A, Sandomenico A, Foca G, Caporale A, Doti N, Iaccarino E, Leonardi A, D'Auria G, Ruvo M, Falcigno L

BIOORGAN MED CHEM (ISSN: 0968-0896), 2017 Oct 28; N/D: N/D-N/D.



Tipo di articolo: Journal Article,

Impact factor: 2.881, Impact factor a 5 anni: 2.911

Url: Non disponibile.

Parole chiave: Binding, Group Epitope Mapping, Tgf-Beta,

Affiliazioni:

*** IBB - CNR ***
CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy., Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy., DISTABIF, Universita degli Studi della Campania "Lugi Vanvitelli", via Vivaldi, 43, 80100 Caserta, Italy., Dept. Medicina Molecolare e Biotecnologie Mediche, Universita Federico II di Napoli, Naples, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy; Dept. of Pharmacy, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy. Electronic address: menotti.ruvo@unina.it., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy; Dept. of Pharmacy, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy. Electronic address: lucia.falcigno@unina.it.,


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Nodal is a growth factor expressed during early embryonic development, but reactivated in several advanced-stage cancers. Targeting of Nodal signaling, which occurs via the binding to Cripto-1 co-receptor, results in inhibition of cell aggressiveness and reduced tumor growth. The Nodal binding region to Cripto-1 was identified and targeted with a high affinity monoclonal antibody (3D1). By STD-NMR technique, we investigated the interaction of Nodal fragments with 3D1 with the aim to elucidate at atomic level the interaction surface. Data indicate with high accuracy the antibody-antigen contact atoms and confirm the information previously obtained by immune-enzymatic methods. Main residues contacted by 3D1 are P46, V47, E49 and E50, which belong to the Nodal loop involved in the interaction with the co-receptor.
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Calvanese L, Sandomenico A, Caporale A, Foca A, Foca G, D'Auria G, Falcigno L, Ruvo M
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3 Records (2 escludendo Abstract e Conferenze).
Impact factor totale: 11.866 (11.866 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 12.577 (12.577 escludendo Abstract e Conferenze).







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