Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy
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Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy (202 visite)

Calvanese L, Foca A, Sandomenico A, Foca G, Caporale A, Doti N, Iaccarino E, Leonardi A, D'Auria G, Ruvo M, Falcigno L

Bioorg Med Chem (ISSN: 0968-0896, 1464-3391), 2017 Oct 28; N/D: N/D-N/D.



Tipo di articolo: Journal Article,

Impact factor: 2.881, Impact factor a 5 anni: 2.911

Url: Non disponibile.

Parole chiave: Binding, Group Epitope Mapping, Tgf-Beta,

Affiliazioni:

*** IBB - CNR ***
CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy., Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy., DISTABIF, Universita degli Studi della Campania "Lugi Vanvitelli", via Vivaldi, 43, 80100 Caserta, Italy., Dept. Medicina Molecolare e Biotecnologie Mediche, Universita Federico II di Napoli, Naples, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy; Dept. of Pharmacy, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy. Electronic address: menotti.ruvo@unina.it., CIRPeB, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy; Institute of Biostructures and Bioimaging, National Research Council, IBB-CNR, via Mezzocannone, 16, 80134 Napoli, Italy; Dept. of Pharmacy, University of Naples Federico II, via Mezzocannone, 16, 80134 Napoli, Italy. Electronic address: lucia.falcigno@unina.it.,


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Nodal is a growth factor expressed during early embryonic development, but reactivated in several advanced-stage cancers. Targeting of Nodal signaling, which occurs via the binding to Cripto-1 co-receptor, results in inhibition of cell aggressiveness and reduced tumor growth. The Nodal binding region to Cripto-1 was identified and targeted with a high affinity monoclonal antibody (3D1). By STD-NMR technique, we investigated the interaction of Nodal fragments with 3D1 with the aim to elucidate at atomic level the interaction surface. Data indicate with high accuracy the antibody-antigen contact atoms and confirm the information previously obtained by immune-enzymatic methods. Main residues contacted by 3D1 are P46, V47, E49 and E50, which belong to the Nodal loop involved in the interaction with the co-receptor.
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Ferrucci V, De Antonellis P, Pennino FP, Asadzadeh F, Virgilio A, Montanaro D, Galeone A, Boffa I, Pisano I, Scognamiglio I, Navas L, Diana D, Pedone E, Gargiulo S, Gramanzini M, Brunetti A, Danielson L, Carotenuto M, Liguori L, Verrico A, Quaglietta L, Errico ME, Del Monaco V, D'Argenio V, Tirone F, Mastronuzzi A, Donofrio V, Giangaspero F, Picard D, Remke M, Garzia L, Daniels C, Delattre O, Swartling FJ, Weiss WA, Salvatore F, Fattorusso R, Chesler L, Taylor MD, Cinalli G, Zollo M
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Calvanese L, Sandomenico A, Caporale A, Foca A, Foca G, D'Auria G, Falcigno L, Ruvo M
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3 Records (2 escludendo Abstract e Conferenze).
Impact factor totale: 11.866 (11.866 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 12.577 (12.577 escludendo Abstract e Conferenze).







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