Progetti in evidenza
Fondo Europeo Pesca(416 visite)
Fondazione Veronesi(Link esterno)
MFAG Grant(987 visite)
Specific Recognition of G-Quadruplexes Over Duplex-DNA by a Macromolecular NIR Two-Photon Fluorescent Probe (20 visite)
J Phys Chem Lett (ISSN: 1948-7185electronic, 1948-7185linking), 2017 Dec 7; 8(23): 5915-5920.
Tipo di articolo: Journal Article
Impact factor: 6.687
Impact factor a 5 anni: 6.91
Parole chiave: Non disponibili.
Url: Non disponibile.
The implication of guanine-rich DNA sequences in biologically important roles such as telomerase dysfunction and the regulation of gene expression has prompted the search for structure-specific G-quadruplex agents for targeted diagnostic and therapeutic applications. Herein, we report on a near-infrared (NIR) two-photon poly(cationic) anthracene-based macromolecule able to selectively target G-quadruplexes (G4s) over genomic double-stranded DNA. In particular, the striking changes in its linear and third-order nonlinear optical properties, combined with the emergence of a strong induced electronic circular dichroism (ECD) signal upon binding to canonical and noncanonical DNA secondary structures allowed for a highly specific detection of several different G4s. Furthermore, through a detailed computational analysis we bring compelling evidence that our probe intercalation within G4s is a thermodynamically favored event, and we fully rationalize the spectroscopic evolution resulting from this complexation event by providing a reasonable explanation regarding the origin of the peculiar ECD effect that accompanies it.
*** IBB - CNR ***
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Science and Technology , Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland., Universite Lyon, ENS de Lyon, CNRS UMR 5182, Universite Lyon 1 , Laboratoire de Chimie, F69342, Lyon, France., Consiglio Nazionale delle Ricerche, Istituto di Biostrutture e Bioimmagini , 80134 Naples, Italy., LIDYL, CEA, CNRS, Universite Paris-Saclay , F-91191 Gif-sur-Yvette, France., Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences , Rudolfa Weigla 12, 53-114 Wroclaw, Poland.,
Esporta in BibTeX Esporta in EndNote
Ultima modifica di Gennaro Angrisano in data Thursday 04 January 2018, 15:14:46
20 visite. Ultima visita in data Wednesday 09 May 2018, 1:17:02
Nessun bando in corso.
Archivio Concorsi>>> (889 visite)
Nessun evento in programma.
Archivio Eventi>>> (780 visite)