A De Novo Heterodimeric Due Ferri Protein Minimizes the Release of Reactive Intermediates in Dioxygen-Dependent Oxidation

A De Novo Heterodimeric Due Ferri Protein Minimizes the Release of Reactive Intermediates in Dioxygen-Dependent Oxidation (165 views visite) (PDF public pubblico 152 views visite)

Chino M, Leone L, Maglio O, D'Alonzo D, Pirro F, Pavone V, Nastri F, Lombardi A

Angew Chem Int Ed (ISSN: 1433-7851, 1521-3773electronic), 2017 Dec 4; 56(49): 15580-15583.

Paper type Tipo di articolo: Journal Article,

Impact factor: 12.102, 5-year impact factor Impact factor a 5 anni: 11.954

Url: Not available. Non disponibile.

Keywords Parole chiave: Bioinorganic Chemistry, Click Chemistry, De Novo Protein Design, Diiron-Oxo Proteins, Oxidoreductase,

Affiliations Affiliazioni:

*** IBB - CNR ***
Department of Chemical Sciences, University of Napoli "Federico II", Via Cintia, 80126, Napoli, Italy., IBB-National Research Council, Via Mezzocannone 16, 80134, Napoli, Italy.,

References Riferimenti:

Not available. Non disponibile.

Metalloproteins utilize O2 as an oxidant, and they often achieve a 4-electron reduction without H2 O2 or oxygen radical release. Several proteins have been designed to catalyze one or two-electron oxidative chemistry, but the de novo design of a protein that catalyzes the net 4-electron reduction of O2 has not been reported yet. We report the construction of a diiron-binding four-helix bundle, made up of two different covalently linked alpha2 monomers, through click chemistry. Surprisingly, the prototype protein, DF-C1, showed a large divergence in its reactivity from earlier DFs (DF: due ferri, two iron). DFs release the quinone imine and free H2 O2 in the oxidation of 4-aminophenol in the presence of O2 , whereas Fe(III) -DF-C1 sequesters the quinone imine into the active site, and catalyzes inside the scaffold an oxidative coupling between oxidized and reduced 4-aminophenol. The asymmetry of the scaffold allowed a fine-engineering of the substrate binding pocket, that ensures selectivity.

Your query Interrogazione effettuata: [atitle, adesc, keywords] "Bioinorganic Chemistry" OR [atitle, adesc, keywords] "Click Chemistry" OR [atitle, adesc, keywords] "De Novo Protein Design" OR [atitle, adesc, keywords] "Diiron-Oxo Proteins" OR [atitle, adesc, keywords] "Oxidoreductase"

▼ Structural characterization of proteins and enzymes isolated from organisms living under unusual conditions

A De Novo Heterodimeric Due Ferri Protein Minimizes the Release of Reactive Intermediates in Dioxygen‐Dependent Oxidation (public domain dominio pubblico) (152 views visite)

Chemistry of Lipid A: At the Heart of Innate Immunity (restricted access accesso riservato) (266 views visite)

Design and engineering of artificial oxygen-activating metalloenzymes (public domain dominio pubblico) (155 views visite)

Designing Covalently Linked Heterodimeric Four-Helix Bundles (public domain dominio pubblico) (156 views visite)

Elucidating the role of the pLG72 R30K substitution in schizophreniasusceptibility (public domain dominio pubblico) (139 views visite)

Spectroscopic and SEM evidences for G4-DNA binding by a synthetic alkyne-containing amino acid with anticancer activity (public domain dominio pubblico) (52 views visite)

Thermus thermophilus as source of thermozymes for biotechnologicalapplications: homologous expression and biochemical characterization of anα-galactosidase. (public domain dominio pubblico) (147 views visite)

Ultrasonographic and multimodal imaging of pediatric genital female diseases (public domain dominio pubblico) (78 views visite)

Your query Interrogazione effettuata: [btitle, keywords] "Bioinorganic Chemistry" [btitle, keywords] "Click Chemistry" [btitle, keywords] "De Novo Protein Design" [btitle, keywords] "Diiron-Oxo Proteins" [btitle, keywords] "Oxidoreductase"

Vai a fine pagina Versione per copia e incolla facile Esporta tutto in BibTeX Esporta tutto in EndNote

Bocian A, Szymańska M, Brykczyńska D, Kubicki M, Wałęsa-chorab M, Roviello GN, Fik-jaskółka MA, Gorczyński A, Patroniak V
* New artificial biomimetic enzyme analogues based on iron(II/III) Schiff Base complexes: an effect of (benz)imidazole organic moieties on phenoxazinone synthase and DNA recognition (129 visite)
Molecules (ISSN: 1420-3049linking, 1420-3049electronic), 2019 Aug 31; 24(17): 3173-3173.
Impact Factor: 3.06
Dettagli    Esporta in BibTeX    Esporta in EndNote

Chino M, Zhang SQ, Pirro F, Leone L, Maglio O, Lombardi A, Degrado WF
* Spectroscopic and metal binding properties of a de novo metalloprotein binding a tetrazinc cluster (239 visite)
Biopolymers (ISSN: 1097-0282electronic, 0006-3525linking, 0006-3525print), 2018 Sep 11; 109(10): e23339-e23339.
Impact Factor: 2.572
Dettagli    Esporta in BibTeX    Esporta in EndNote

Chino M, Leone L, Zambrano G, Pirro F, D'Alonzo D, Firpo V, Aref D, Lista L, Maglio O, Nastri F, Lombardi A
* Oxidation catalysis by iron and manganese porphyrins within enzyme-like cages (128 visite)
Biopolymers (ISSN: 0006-3525linking, 0006-3525print, 1097-0282electronic), 2018 Aug; 109(10): e23107-e23107.
Impact Factor: 2.572
Dettagli    Esporta in BibTeX    Esporta in EndNote

Marchiori MF, Riul TB, Oliveira Bortot L, Andrade P, Junqueira GG, Foca G, Doti N, Ruvo M, Dias-baruffi M, Carvalho I, Campo VL
* Binding of triazole-linked galactosyl arylsulfonamides to galectin-3 affects Trypanosoma cruzi cell invasion (323 visite)
Bioorg Med Chem (ISSN: 0968-0896, 1464-3391), 2017 Nov 1; 25(21): 6049-6059.
Impact Factor: 2.881
Dettagli    Esporta in BibTeX    Esporta in EndNote

Troise AD, Buonanno M, Fiore A, Monti SM, Fogliano V
* Evolution of protein bound Maillard reaction end-products and free Amadori compounds in low lactose milk in presence of fructosamine oxidase (217 visite)
Food Chem (ISSN: 0308-8146, 0308-8146print), 2016 Dec 1; 212: 722-729.
Impact Factor: 4.529
Dettagli    Esporta in BibTeX    Esporta in EndNote

Monti SM, De Simone G, D'Ambrosio K
* L-Histidinol Dehydrogenase as a New Target for Old Diseases (235 visite)
Curr Top Med Chem (ISSN: 1568-0266, 1568-0266linking), 2016 Apr 13; 16(21): 2369-2378.
Impact Factor: 2.864
Dettagli    Esporta in BibTeX    Esporta in EndNote

Celentano V, Diana D, Di Salvo C, De Rosa L, Romanelli A, Fattorusso R, D'Andrea LD
* 1, 2, 3-Triazole Bridge as Conformational Constrain in beta-Hairpin Peptides: Analysis of Hydrogen-Bonded Positions (305 visite)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2016 Apr 11; 22(16): 5534-5537.
Impact Factor: 5.317
Dettagli    Esporta in BibTeX    Esporta in EndNote

Messori L, Merlino A
* Cisplatin binding to proteins: A structural perspective (253 visite)
Coord Chem Rev (ISSN: 0010-8545), 2016; 315: 67-89.
Impact Factor: 13.324
Dettagli    Esporta in BibTeX    Esporta in EndNote

Chino M, Leone L, Maglio O, Lombardi A
* Designing Covalently Linked Heterodimeric Four-Helix Bundles (223 visite) (PDF 156 visite)
Method Enzymol (ISSN: 0076-6879, 1557-7988electronic), 2016; 580: 471-499.
Impact Factor: 1.972
Dettagli    Esporta in BibTeX    Esporta in EndNote

Trusso Sfrazzetto G, Satriano C, Tomaselli GA, Rizzarelli E
* Synthetic fluorescent probes to map metallostasis and intracellular fate of zinc and copper (254 visite)
Coord Chem Rev (ISSN: 0010-8545), 2016; 311: 125-167.
Impact Factor: 13.324
Dettagli    Esporta in BibTeX    Esporta in EndNote

Ringhieri P, Diaferia C, Galdiero S, Palumbo R, Morelli G, Accardo A
* Liposomal doxorubicin doubly functionalized with CCK8 and R8 peptide sequences for selective intracellular drug delivery (359 visite)
J Pept Sci (ISSN: 1075-2617, 1099-1387, 1075-2617linking), 2015; 21(5): 415-425.
Impact Factor: 1.951
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, Fiorentino G, Pirone L, Contursi P, Bartolucci S, Limauro D
* Functional and structural characterization of protein disulfide oxidoreductase from Thermus thermophilus HB27 (234 visite)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2014 Jul; 18(4): 723-731.
Impact Factor: 2.306
Dettagli    Esporta in BibTeX    Esporta in EndNote

Limauro D, De Simone G, Pirone L, Bartolucci S, D'Ambrosio K, Pedone E
* Sulfolobus solfataricus thiol redox puzzle: characterization of an atypical protein disulfide oxidoreductase (303 visite)
Extremophiles (ISSN: 1431-0651, 1433-4909, 1433-4909electronic), 2014 Mar; 18(2): 219-228.
Impact Factor: 2.306
Dettagli    Esporta in BibTeX    Esporta in EndNote

D'Ambrosio K, Lopez M, Dathan NA, Ouahrani-bettache S, Köhler S, Ascione G, Monti SM, Winum JY, De Simone G
* Structural basis for the rational design of new anti-Brucella agents: The crystal structure of the C366S mutant of l-histidinol dehydrogenase from Brucella suis (345 visite)
Biochimie (ISSN: 0300-9084, 1638-6183, 0300-9084linking), 2014 Feb; 97(1): 114-120.
Impact Factor: 2.963
Dettagli    Esporta in BibTeX    Esporta in EndNote

Perrino C, Schiattarella GG, Sannino A, Pironti G, Petretta MP, Cannavo A, Gargiulo G, Ilardi F, Magliulo F, Franzone A, Carotenuto G, Serino F, Altobelli GG, Cimini V, Cuocolo A, Lombardi A, Goglia F, Indolfi C, Trimarco B, Esposito G
* Genetic deletion of uncoupling protein 3 exaggerates apoptotic cell death in the ischemic heart leading to heart failure (336 visite)
J Am Heart Assoc Journal Of The American Heart Association (ISSN: 2047-9980), 2013 May 20; 2(3): e000086-e000086.
Impact Factor: 2.882
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pennacchio A, Sannino V, Sorrentino G, Rossi M, Raia CA, Esposito L
* Biochemical and structural characterization of recombinant short-chain NAD(H)-dependent dehydrogenase/reductase from Sulfolobus acidocaldarius highly enantioselective on diaryl diketone benzil (306 visite)
Appl Microbiol Biot (ISSN: 0175-7598), 2013 May; 97(9): 1-16.
Impact Factor: 3.811
Dettagli    Esporta in BibTeX    Esporta in EndNote

Accardo A, Ringhieri P, Tesauro D, Morelli G
* Liposomes derivatized with tetrabranched neurotensin peptides via click chemistry reactions (326 visite)
New J Chem (ISSN: 1144-0546), 2013; 37(11): 3528-3534.
Impact Factor: 3.159
Dettagli    Esporta in BibTeX    Esporta in EndNote

Faiella M, Maglio O, Nastri F, Lombardi A, Lista L, Hagen WR, Pavone V
* De Novo Design, Synthesis and Characterisation of MP3, A New Catalytic Four-Helix Bundle Hemeprotein (352 visite)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2012 Dec 7; 18(50): 15960-15971.
Impact Factor: 5.831
Dettagli    Esporta in BibTeX    Esporta in EndNote

Tarallo R, Accardo A, Falanga A, Guarnieri D, Vitiello G, Netti P, D'Errico G, Morelli G, Galdiero S
* Clickable functionalization of liposomes with the gH625 peptide from herpes simplex virus type i for intracellular drug delivery (356 visite)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2011 Nov 4; 17(45): 12659-12668.
Impact Factor: 5.925
Dettagli    Esporta in BibTeX    Esporta in EndNote

Nastri F, Lista L, Ringhieri P, Vitale R, Faiella M, Andreozzi C, Travascio P, Maglio O, Lombardi A, Pavone V
* A Heme-Peptide Metalloenzyme Mimetic with Natural Peroxidase-Like Activity (346 visite)
Chemistry (ISSN: 0947-6539, 1521-3765, 1521-3765electronic), 2011 Apr; 17(16): 4444-4453.
Impact Factor: 5.925
Dettagli    Esporta in BibTeX    Esporta in EndNote

Kállay C, Turi I, Timári S, Nagy Z, Sanna D, Pappalardo G, De Bona P, Rizzarelli E, Sóvágó I
* The effect of point mutations on copper(II) complexes with peptide fragments encompassing the 106-114 region of human prion protein (415 visite)
Monatshefte Fur Chemie (ISSN: 0026-9247), 2011 Apr; 142(4): 411-419.
Impact Factor: 1.532
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, Limauro D, D'Ambrosio K, De Simone G, Bartolucci S
* Multiple catalytically active thioredoxin folds: a winning strategy for many functions (370 visite)
Cell Mol Life Sci Cellular And Molecular Life Sciences (ISSN: 1420-682x), 2010 Nov; 67(22): 3797-3814.
Impact Factor: 7.047
Dettagli    Esporta in BibTeX    Esporta in EndNote

Limauro D, D'Ambrosio K, Langella E, De Simone G, Galdi I, Pedone C, Pedone E, Bartolucci S
* Exploring the catalytic mechanism of the first dimeric Bcp: Functional, structural and docking analyses of Bcp4 from Sulfolobus solfataricus (227 visite)
Biochimie (ISSN: 1638-6183, 0300-9084, 1638-6183electronic), 2010 Oct; 92(10): 1435-1444.
Impact Factor: 3.787
Dettagli    Esporta in BibTeX    Esporta in EndNote

Scir A, Pedone E, Ausili A, Saviano M, Baldassarre M, Bertoli E, Bartolucci S, Tanfani F
* High hydrostatic pressure-induced conformational changes in protein disulfide oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. A Fourier-transform infrared spectroscopic study (264 visite)
Molecular Biosystems (ISSN: 1742-206x), 2010; 6(10): 2015-2022.
Impact Factor: 3.825
Dettagli    Esporta in BibTeX    Esporta in EndNote

Ruggiero A, Masullo M, Ruocco MR, Grimaldi P, Lanzotti MA, Arcari P, Zagari A, Vitagliano L
* Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: A thermostable protein with two functions (282 visite)
Bba-Gen Subjects (ISSN: 1570-9639, 0006-3002, 0925-4439), 2009 Mar; 1794(3): 554-562.
Impact Factor: 2.48
Dettagli    Esporta in BibTeX    Esporta in EndNote

Limauro D, Saviano M, Galdi I, Rossi M, Bartolucci S, Pedone E
* Sulfolobus solfataricus protein disulphide oxidoreductase: insight into the roles of its redox sites (163 visite)
Protein Engineering Design & Selection (ISSN: 1741-0126), 2009 Jan; 22(1): 19-26.
Impact Factor: 2.596
Dettagli    Esporta in BibTeX    Esporta in EndNote

Limauro D, Pedone E, Galdi I, Bartolucci S
* Peroxiredoxins as cellular guardians in Sulfolobus solfataricus - characterization of Bcp1, Bcp3 and Bcp4 (274 visite)
Febs Journal (ISSN: 1742-464x), 2008 May; 275(9): 2067-2077.
Impact Factor: 3.139
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, Limauro D, D'Alterio R, Rossi M, Bartolucci S
* Characterization of a multifunctional protein disulfide oxidoreductase from Sulfolobus solfataricus (211 visite)
Febs Journal (ISSN: 1742-464x), 2006 Dec; 273(23): 5407-5420.
Impact Factor: 3.033
Dettagli    Esporta in BibTeX    Esporta in EndNote

D'Ambrosio K, Pedone E, Langella E, De Simone G, Rossi M, Pedone C, Bartolucci S
* A novel member of the protein disulfide oxidoreductase family from Aeropyrum pernix K1: Structure, function and electrostatics (280 visite)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2006 Sep 29; 362(4): 743-752.
Impact Factor: 4.89
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, D'Ambrosio K, De Simone G, Rossi M, Pedone C, Bartolucci S
* Insights on a new PDI-like family: Structural and functional analysis of a protein disulfide oxidoreductase from the bacterium Aquifex aeolicus (281 visite)
J Mol Biol (ISSN: 0022-2836, 1089-8638, 1089-8638electronic), 2006 Feb 10; 356(1): 155-164.
Impact Factor: 4.89
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, Saviano M, Bartolucci S, Rossi M, Ausili A, Scire A, Bertoli E, Tanfani F
* Temperature-, SDS-, and pH-induced conformational changes in protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus: A dynamic simulation and fourier transform infrared spectroscopic study (239 visite)
J Proteome Res (ISSN: 1535-3893), 2005 Nov; 4(6): 1972-1980.
Impact Factor: 6.901
Dettagli    Esporta in BibTeX    Esporta in EndNote

D'Ambrosio K, De Simone G, Pedone E, Rossi M, Bartolucci S, Pedone C
* Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aeropyrum pernix K1 (268 visite)
Nucleosides Nucleotides Nucleic Acids (ISSN: 1744-3091, 1744-4309), 2005 Mar 1; 61(3): 335-336.
Impact Factor: 0.568
Dettagli    Esporta in BibTeX    Esporta in EndNote

Calhoun JR, Nastri F, Maglio O, Pavone V, Lombardi A, Degrado WF
* Artificial diiron proteins: From structure to function (390 visite)
Biopolymers (ISSN: 0006-3525, 0006-6352, 0006-3525print), 2005; 80(2-3): 264-278.
Impact Factor: 2.545
Dettagli    Esporta in BibTeX    Esporta in EndNote

D'Ambrosio K, De Simone G, Pedone E, Rossi M, Bartolucci S, Pedone C
* Crystallization and preliminary X-ray diffraction studies of a protein disulfide oxidoreductase from Aquifex aeolicus (247 visite)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2004 Nov; 60(11): 2076-2077.
Impact Factor: 1.693
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, Bartolucci S, Fiorentino G
* Sensing and adapting to environmental stress: The Archaeal tactic (237 visite)
Frontiers In Bioscience (ISSN: 1093-9946, 1093-4715), 2004 Sep 1; 9: 2909-2926.
Impact Factor: 3.226
Dettagli    Esporta in BibTeX    Esporta in EndNote

Pedone E, Ren B, Ladenstein R, Rossi M, Bartolucci S
* Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus - A member of a novel protein family related to protein disulfide-isomerase (229 visite)
Eur J Biochem (ISSN: 0014-2956, 0014-2956print), 2004 Sep; 271(16): 3437-3448.
Impact Factor: 3.26
Dettagli    Esporta in BibTeX    Esporta in EndNote

D'Ambrosio K, Kauffmann B, Rouhier N, Benedetti E, Jacquot JP, Aubry A, Corbier C
* Crystallization and preliminary X-ray studies of the glutaredoxin from poplar in complex with glutathione (200 visite)
Acta Crystallogr D Biol Crystallogr (ISSN: 0907-4449, 0907-4449linking), 2003 Jun; 59: 1043-1045.
Impact Factor: 2.208
Dettagli    Esporta in BibTeX    Esporta in EndNote

Garzillo AM, Colao MC, Buonocore V, Oliva R, Falcigno L, Saviano M, Santoro AM, Zappala R, Bonomo RP, Bianco C, Giardina P, Palmieri G, Sannia G
* Structural and kinetic characterization of native laccases from Pleurotas ostreatus, Rigidoporus lignosus, and Trametes trogii (290 visite)
Journal Of Protein Chemistry (ISSN: 0277-8033), 2001 Apr; 20(3): 191-201.
Impact Factor: 1.021
Dettagli    Esporta in BibTeX    Esporta in EndNote

Bonomo RP, Cennamo G, Purrello R, Santoro AM, Zappala R
* Comparison Of Three Fungal Laccases From Rigidoporus Lignosus And Pleurotus Ostreatus: Correlation Between Conformation Changes And Catalytic Activity (249 visite)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 2001 Jan 1; 83(1): 67-75.
Impact Factor: 1.729
Dettagli    Esporta in BibTeX    Esporta in EndNote

Cambria M, Cambria A, Ragusa S, Rizzarelli E
Production, purification, and properties of an extracellular laccase from Rigidoporus lignosus (220 visite)
Protein Expr Purif (ISSN: 1046-5928), 2000 Mar; 18(2): 141-147.
Impact Factor: 1.569
Dettagli    Esporta in BibTeX    Esporta in EndNote

Bonomo RP, Boudet AM, Cozzolino R, Rizzarelli E, Santoro AM, Sterjiades R, Zappalà R
A comparative study of two isoforms of laccase secreted by the 'white- rot' fungus Rigidoporus lignosus, exhibiting significant structural and functional differences (221 visite)
J Chem Res (ISSN: 0162-0134, 1873-3344, 0162-0134print), 1998 Sep; 71(3-4): 205-211.
Impact Factor: 1.162
Dettagli    Esporta in BibTeX    Esporta in EndNote

41 Records (40 escludendo Abstract e Conferenze).
Impact factor totale: 152.575 (149.693 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 170.345 (167.463 escludendo Abstract e Conferenze).

Versione per copia e incolla facile Esporta tutto in BibTeX Esporta tutto in EndNote

Vai a inizio pagina

Export to Esporta in BibTeX Export to Esporta in EndNote

Last modified by Ultima modifica di Gennaro Angrisano on in data Sunday 12 July 2020, 13:14:42
165 views visite. Last view on Ultima visita in data Monday 23 November 2020, 0:04:04

Copyright 2020 © CNR Istituto di Biostrutture e Bioimmagini