Keywords Parole chiave: Aquomet, Bis-Hystidyl, Crystallography, Hemoglobin, X-Ray
, Disulfides Chemistry
, Heme Chemistry
, Hemoglobins Chemistry
, Humans
, Models, Molecular
, Protein Structure, Quaternary
, Protein Subunits Chemistry
Affiliations Affiliazioni: *** IBB - CNR ***
Department of Chemical Sciences, University of Naples "Federico II", Naples, Italy
Accademia di Scienze Fisiche e Matematiche della Societa Nazionale di Scienze, Lettere ed Arti in Napoli, Naples, Italy.
Institute of Biostructures and Bioimaging, C.N.R., Naples, Italy.
CEINGE, Napoli, Italy. Electronic address: avergara@unina.it.
References Riferimenti: Not available. Non disponibili.
Crystal structure of the ferric homotetrameric beta4 human hemoglobin
Spectroscopic studies carried out in the early seventies have shown that the beta-homotetramer of human hemoglobin (beta4-HbA) in the ferric state is a mixture of aquomet and bis-histidyl forms. Here we present the first crystal structure, solved at 2.10A resolution, of the oxidized form of beta4-HbA. The overall quaternary structure of the protein in the ferric state is virtually indistinguishable from that of the ferrous deoxygenated and carbomonoxy forms. The structure reveals that the four hemes are exclusively in an aquomet coordination, without any trace of bis-histidyl coordination. The oxidation of beta4-HbA is associated with the formation of a disulfide bridge between residues Cys112(G14) of beta1/beta4 and beta2/beta3 chains. The coordination state of beta4-HbA has been compared to that known for other organisms that exhibit bis-histidyl heme coordination in the beta4 state. This occurrence has been discussed in terms of different organism physiology.
Crystal structure of the ferric homotetrameric beta4 human hemoglobin
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ * Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer(211 visite) N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756. Impact Factor:59.558 DettagliEsporta in BibTeXEsporta in EndNote
Testino G, Leone S, Fagoonee S, Del Bas JM, Rodriguez B, Puiggros F, Marine S, Rodriguez MA, Morina D, Armengol L, Caimari A, Arola L, Cimini FA, Barchetta I, Carotti S, Bertoccini L, Baroni MG, Vespasiani-gentilucci U, Cavallo MG, Morini S, Nelson JE, Roth CL, Wilson LA, Yates KP, Aouizerat B, Morgan-stevenson V, Whalen E, Hoofnagle A, Mason M, Gersuk V, Yeh MM, Kowdley KV, Lee SM, Jun DW, Cho YK, Jang KS, Kucukazman M, Ata N, Dal K, Yeniova AO, Kefeli A, Basyigit S, Aktas B, Akin KO, Agladioglu K, Ure OS, Topal F, Nazligul Y, Beyan E, Ertugrul DT, Catena C, Cosma C, Camozzi V, Plebani M, Ermani M, Sechi LA, Fallo F, Goto Y, Ray MB, Mendenhall CL, French SW, Gartside PS Serum vitamin A deficiency and increased intrahepatic expression of cytokeratin antigen in alcoholic liver disease(427 visite) Hepatology (ISSN: 1827-1669electronic, 0026-4806linking), 1988 Sep; 83120693611123109(5): 1019-1026. Impact Factor:0.913 DettagliEsporta in BibTeXEsporta in EndNote
630 Records (611 escludendo Abstract e Conferenze). Impact factor totale: 2484.999 (2399.708 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 2533.537 (2417.782 escludendo Abstract e Conferenze).
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