Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation(118 views visite) Pirone L, Pitzer JE, D'Abrosca G, Fattorusso R, Malgieri G, Pedone EM, Pedone PV, Roop RM, Baglivo I
Sci Rep (ISSN: 2045-2322electronic, 2045-2322linking), 2018 Nov 22; 8(1): 17238-17238.
Institute of Biostructures and Bioimaging, C.N.R, Naples, 80134, Italy., Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA., Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy., Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy. paolov.pedone@unicampania.it., Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA. ROOPR@ecu.edu., Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy. ilaria.baglivo@unicampania.it.,
Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy.
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Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation
MucR is a member of the Ros/MucR family of prokaryotic zinc-finger proteins found in the alpha-proteobacteria which regulate the expression of genes required for the successful pathogenic and symbiotic interactions of these bacteria with the eukaryotic hosts. The structure and function of their distinctive zinc-finger domain has been well-studied, but only recently the quaternary structure of the full length proteins was investigated demonstrating their ability to form higher-order oligomers. The aim of this study was to identify the region of MucR involved in higher-order oligomer formation by analysing deletion and point mutants of this protein by Light Scattering, and to determine the role that MucR oligomerization plays in the regulatory function of this protein. Here we demonstrate that a conserved hydrophobic region at the N-terminus of MucR is responsible for higher-order oligomer formation and that MucR oligomerization is essential for its regulatory function in Brucella. All these features of MucR are shared by the histone-like nucleoid structuring protein, (H-NS), leading us to propose that the prokaryotic zinc-finger proteins in the MucR/Ros family control gene expression employing a mechanism similar to that used by the H-NS proteins, rather than working as classical transcriptional regulators.
Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation
Santulli G, Cipolletta E, Sorriento D, Del Giudice C, Anastasio A, Monaco S, Maione AS, Condorelli G, Puca A, Trimarco B, Illario M, Iaccarino G * CaMK4 gene deletion induces hypertension(284 visite) J Am Heart Assoc Journal Of The American Heart Association (ISSN: 2047-9980), 2012; 1(4): N/D-N/D. Impact Factor:2.882 DettagliEsporta in BibTeXEsporta in EndNote
70 Records (62 escludendo Abstract e Conferenze). Impact factor totale: 319.928 (286.209 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 324.834 (290.836 escludendo Abstract e Conferenze).
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