New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes
New G-quadruplex-forming oligodeoxynucleotides incorporating a bifunctional Double-Ended Linker (DEL): effects of DEL size and ODNs orientation on the topology, stability and molecularity of DEL-G-quadruplexes(83 visite) Marzano M, Falanga AP, D'Errico S, Pinto B, Roviello GN, Piccialli G, Oliviero G, Borbone N
Molecules (ISSN: 1420-3049linking, 1420-3049electronic), 2019 Feb 12; 24(3): 654-654.
*** IBB - CNR *** Dipartimento di Farmacia, Universita degli Studi di Napoli Federico II, via Domenico Montesano 49, 80131 Napoli, Italy. firstname.lastname@example.org. Dipartimento di Chimica, Universita degli Studi di Milano, via Camillo Golgi 19, 20133 Milano, Italy. email@example.com. Istituto di Biostrutture e Bioimmagini, CNR, Via Tommaso De Amicis 95, 80145 Napoli, Italy. firstname.lastname@example.org. Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universita degli Studi di Napoli Federico II, via Sergio Pansini 5, 80131 Napoli, Italy. email@example.com.
G-quadruplexes (G4s) are unusual secondary structures of DNA occurring <br>in guanosine-rich oligodeoxynucleotide (ODN) strands that are <br>extensively studied for their relevance to the biological processes in <br>which they are involved. In this study, we report the synthesis of a new<br> kind of G4-forming molecule named double-ended-linker ODN (DEL-ODN), in<br> which two TG<sub>4</sub>T strands are attached to the two ends of <br>symmetric, non-nucleotide linkers. Four DEL-ODNs differing for the <br>incorporation of either a short or long linker and the directionality of<br> the TG<sub>4</sub>T strands were synthesized, and their ability to form<br> G4 structures and/or multimeric species was investigated by PAGE, <br>HPLC–size-exclusion chromatography (HPLC–SEC), circular dichroism (CD), <br>and NMR studies in comparison with the previously reported monomeric <br>tetra-ended-linker (TEL) analogues and with the corresponding <br>tetramolecular species (TG<sub>4</sub>T)<sub>4</sub>. The structural <br>characterization of DEL-ODNs confirmed the formation of stable, <br>bimolecular DEL-G4s for all DEL-ODNs, as well as of additional DEL-G4 <br>multimers with higher molecular weights, thus suggesting a way towards <br>the obtainment of thermally stable DNA nanostructures based on <br>reticulated DEL-G4s