The influence of probiotic diet and chondroitin sulfate administration on Ptgs2, Tgfb1 and Col2a1 expression in rat knee cartilage during monoiodoacetate-induced osteoarthritis(47 visite) Korotkyi OH, Vovk AA, Dranitsina AS, Falalyeyeva TM, Dvorshchenko KO, Fagoonee S, Ostapchenko LI
Tipo di articolo: Journal Article, Comparative Study
Impact factor: 2.863, Impact factor a 5 anni: 1.515
Url: Non disponibile.
Parole chiave: Animals
, Cartilage, Articular Drug Effects Metabolism
, Chondroitin Sulfates Administration, Dosage Therapeutic Use
, Collagen Type Ii Biosynthesis Genetics
, Cyclooxygenase 2 Biosynthesis Genetics
, Drug Evaluation, Preclinical
, Food-Drug Interactions
, Gene Expression Regulation Drug Effects
, Iodoacetic Acid Toxicity
, Osteoarthritis, Knee Chemically Induced Diet Therapy Drug Therapy Metabolism
, Probiotics Therapeutic Use
, Messenger Biosynthesis
, Transforming Growth Factor Beta1 Biosynthesis Genetics
*** IBB - CNR *** Taras Shevchenko National University of Kyiv, Kyiv, Ukraine. Institute of Biostructure and Bioimaging (CNR) c/o Molecular Biotechnology Center, Turin, Italy.
BACKGROUND: Osteoarthritis (OA) is a common worldwide disease induced by a wide range of biochemical processes, mainly inflammation and degradation of collagen. The aim of this study, was to describe the effect of a multistrain probiotic (PB) and chondroitin sulfate (CS), administered separately or in combination, on the expression of Ptgs2, Tgfb1 and Col2a1 during monoiodoacetate-induced OA in male rats. METHODS: OA was induced in male rats by injecting monoiodoacetate in right hind knee. Therapeutic groups received 3 mg/kg of CS for 28 days and/or 1.4 g/kg of multistrain PB for 14 days. Knee cartilage were taken 30 days after monoiodoacetate injection. RNA was extracted and the expression of Ptgs2, Tgfb1 and Col2a1 were analyzed using SYBR Green 1-step real-time quantitative polymerase chain reaction. RESULTS: Induction of OA caused an upregulation in Ptgs2, Tgfb1 expression, and downregulation of Col2a1. Separate administration of PB and CS reduced Ptgs2 and Tgfb1 expressions. Their combined administration significantly decreased the expression of these pro-inflammatory cytokines, comparable to controls. Expression of Col2a1 showed similar behaviour, with upregulation in therapeutic group with separate administration and the cumulative effects in case of co-administration. CONCLUSIONS: The multistrain PB diet may offer a perspective to improve the standard treatment of OA and, necessitates further investigation with clinical trials.<br>
12 Records (10 escludendo Abstract e Conferenze). Impact factor totale: 47.739 (45.271 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 44.905 (42.127 escludendo Abstract e Conferenze).