KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2alpha(121 views visite) Pirone L, Smaldone G, Spinelli R, Barberisi M, Beguinot F, Vitagliano L, Miele C, Di Gaetano S, Raciti GA, Pedone E
Keywords Parole chiave: 3t3-L1 Cells, Ap2alpha, Adipogenesis, Kctd1, Preadipocytes
Affiliations Affiliazioni: *** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Napoli, Italy. IRCSS SDN, Via E. Gianturco, 113, 80143 Napoli, Italy. URT "Genomica del Diabete", Istituto per l'Endocrinologia e l'Oncologia Sperimentale "Gaetano Salvatore", CNR, Napoli, Italy Dipartimento di Scienze Mediche Traslazionali, Universita degli Studi di Napoli Federico II, Italy. Dipartimento Scienze Anastesiologiche, Chirurgiche E Dell'emergenza, Universita Della Campania-Luigi Vanvitelli, Caserta, Italy. IRCCS SDN, Via E. Gianturco, 113, 80143 Napoli, Italy.
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KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2alpha
Adipogenesis has an important role in regulating energy balance, tissue homeostasis and disease pathogenesis. 3T3-L1 preadipocytes have been widely used as an in vitro model for studying adipocyte differentiation. We here show that KCTD1, a member of the potassium channel containing tetramerization domain proteins, plays an active role in adipogenesis. In particular, we show KCTD1 expression 3T3-L1 cells increases upon adipogenesis induction. Treatment of 3T3-L1 preadipocytes with Kctd1-specific siRNA inhibited the differentiation, as indicated by reduction of expression of the specific adipogenic markers C/ebpalpha, Ppargamma2, Glut4, and Adiponectin. Moreover, we also show that the protein physically interacts with the transcription factor AP2alpha, a known inhibitor of adipogenesis, both in vitro and in cells. Interestingly, our data indicate that KCTD1 promotes adipogenesis through the interaction with AP2alpha and by removing it from the nucleus. Collectively, these findings disclose a novel role for KCTD1 and pave the way for novel strategies aimed at modulating adipogenesis.
KCTD1: A novel modulator of adipogenesis through the interaction with the transcription factor AP2alpha
1 Records (1 escludendo Abstract e Conferenze). Impact factor totale: 4.784 (4.784 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 3.227 (3.227 escludendo Abstract e Conferenze).
Last modified by Ultima modifica di Gennaro Angrisano on in data Sunday 12 July 2020, 13:14:40 121 views visite. Last view on Ultima visita in data Friday 26 February 2021, 10:47:39