Biochemical and Conformational Characterization of Recombinant VEGFR2 Domain "7"(150 views visite) Di Stasi R, Diana D, De Rosa L, Fattorusso R, D'Andrea LD
Keywords Parole chiave: Allosteric Binders, Angiogenesis, Recombinant Expression, Vegf
Affiliations Affiliazioni: *** IBB - CNR ***
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134, Naples, Italy. rossella.distasi@cnr.it. Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Universita della Campania "L. Vanvitelli", Via Vivaldi, 43 - 81100, Caserta, Italy.
References Riferimenti: Not available. Non disponibili.
Biochemical and Conformational Characterization of Recombinant VEGFR2 Domain "7"
Angiogenesis is a biological process finely tuned by a plethora of pro- and anti-angiogenic molecules, among which vascular endothelial growth factors (VEGFs). Their biological activity is expressed through the interaction with three cognate receptor tyrosine kinases, VEGFR1, 2, and 3. VEGFR2 is the primary regulator of angiogenesis. Ligand-induced VEGFR2 dimerization and activation depend on direct ligand binding to extracellular domains 2 and 3 of receptor and in the establishment of interactions between proximal membrane domains. VEGFR2 domain 7 has been shown to play a crucial role in receptor dimerization and regulation, therefore, representing a convenient target for the allosteric modulation of VEGFR2 activity. The ability to prepare a functional VEGFR2D7 domain represents the starting point to the development of novel VEGFR2 binders acting as allosteric inhibitors of receptor activity. Here, we describe a robust and efficient procedure for the preparation in E. coli of the VEGFR2 domain 7. The protein was obtained with a good yield and was properly folded. It was investigated in a biochemical and structural study, providing information on its conformational arrangement and in solution properties.
Biochemical and Conformational Characterization of Recombinant VEGFR2 Domain "7"
Bogdanovich S, Kim Y, Mizutani T, Yasuma R, Tudisco L, Cicatiello V, Bastos-carvalho A, Kerur N, Hirano Y, Baffi JZ, Tarallo V, Li S, Yasuma T, Arpitha P, Fowler BJ, Wright CB, Apicella I, Greco A, Brunetti A, Ruvo M, Sandomenico A, Nozaki M, Ijima R, Kaneko H, Ogura Y, Terasaki H, Ambati BK, Leusen JH, Langdon WY, Clark MR, Armour KL, Bruhns P, Verbeek JS, Gelfand BD, De Falco S, Ambati J * Human IgG1 antibodies suppress angiogenesis in a target-independent manner(483 visite) Signal Transduct Target Ther (ISSN: 2059-3635print), 2016; 1: N/D-N/D. Impact Factor:0 DettagliEsporta in BibTeXEsporta in EndNote
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Ntziachristos V, Cuénod CA, Fournier L, Balvay D, Pradel C, Siauve N, Clement O, Jouannot E, Lucidarme O, Vecchio SD, Salvatore M, Law B, Tung C-H, Jain RK, Fukumura D, Munn LL, Brown EB, Schellenberger E, Montet X, Weissleder R, Clerck ND, Postnov A * Tumor Imaging(293 visite) Textbook Of In Vivo Imaging In Vertebrates (ISSN: 9780-4700), 2007 Jul 16; 1: 277-309. Impact Factor:1.148 DettagliEsporta in BibTeXEsporta in EndNote
65 Records (62 escludendo Abstract e Conferenze). Impact factor totale: 277.948 (274.373 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 276.314 (271.681 escludendo Abstract e Conferenze).
Last modified by Ultima modifica di Marco Comerci on in data Thursday 23 July 2020, 12:31:30 150 views visite. Last view on Ultima visita in data Friday 22 January 2021, 23:56:24