Monoacylglycerides from the Diatom Skeletonema marinoi Induce Selective Cell Death in Cancer Cells(39 visite) Miceli M, Cutignano A, Conte M, Ummarino R, Romanelli A, Ruvo M, Leone M, Mercurio FA, Doti N, Manzo E, Romano G, Altucci L, Ianora A
Mar Drugs (ISSN: 1660-3397linking), 2019 Nov 1; 17
Tipo di articolo: Journal Article
Impact factor: 3.345, Impact factor a 5 anni: 4.031
Url: Non disponibile.
Parole chiave: Ms
, Skeletonema Marinoi
, Bioactive Lipids
, Cytotoxic Activity
*** IBB - CNR *** CEINGE-Biotecnologie Avanzate s.c.ar.l., 80145 Naples, Italy. firstname.lastname@example.org. CNR-Institute of Biomolecular Chemistry, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy. email@example.com. Department of Precision Medicine, University of Campania 'Luigi Vanvitelli', Via L. De Crecchio 7, 80138 Naples, Italy. firstname.lastname@example.org. Institute of Biostructures and Bioimaging (IBB-CNR), Via Mezzocannone 16, 80134 Naples, Italy. email@example.com. Marine Biotechnology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy. firstname.lastname@example.org. Department of Pharmaceutical Sciences, University of Milan, via Venezian 21, 20133 Milan, Italy. email@example.com.
Microalgae are an excellent source of valuable compounds for nutraceutical and cosmeceutical applications. These photosynthesizing microorganisms are amenable for large-scale production, thus overcoming the bottleneck of biomass supply for chemical and activity characterization of bioactive compounds. This characteristic has recently also prompted the screening of microalgae for potential pharmaceutical applications. Here, we show that monoacylglycerides (MAGs) purified from the marine diatom Skeletonema marinoi have selective cytotoxic activity against the haematological cancer cell line U-937 and colon cancer cell line HCT-116 compared to normal MePR-2B cells. LC-MS analysis of the raw extract revealed that in their natural form, MAGs occur as 2-monoacyl derivatives and include mainly C16 and C20 analogues, but they are converted into the corresponding 1-isomers during purification processes. Pure compounds along with the synthetic 1-monoarachidonoylglycerol tested on HCT-116 and U-937 tumor cell lines induced cell death via apoptosis. The mechanism of action was investigated, and we show that it involves the induction of apoptosis through caspase 3/7 activation. These findings pave the way for the possible use of these molecules as potential anticancer agents or as precursors for the generation of new and more potent and selective compounds against tumor cells.<br>