In vivo assessment of tumour associated macrophages in murine melanoma obtained by low-field relaxometry in the presence of iron oxide particles(110 views visite) Baroni S, Ruggiero MR, Bitonto V, Broche LM, Lurie DJ, Aime S, Geninatti Crich S
Biomaterials (ISSN: 0142-9612linking, 1878-5905electronic), 2020 Jan 27; 236: 119805-119805.
Keywords Parole chiave: Intracellular Water Lifetime
, Iron Oxide Particles
, Low Field Relaxometry (nmrd)
, Magnetic Resonance Imaging
, Tumour Associated Macrophages
Affiliations Affiliazioni: *** IBB - CNR ***
Department of Molecular Biotechnology and Health Sciences, University of Torino, Via Nizza 52, Torino, Italy.
Aberdeen Biomedical Imaging Centre, University of Aberdeen, Foresterhill, AB25 2ZD, Aberdeen, UK.
Istituto di Biostrutture e Bioimmagini (IBB), CNR, Torino, Italy.
References Riferimenti: Not available. Non disponibili.
In vivo assessment of tumour associated macrophages in murine melanoma obtained by low-field relaxometry in the presence of iron oxide particles
Tumour-associated macrophages (TAM) are forced by cancer cells to adopt an anti-inflammatory phenotype and secrete factors to promote tumour invasion thus being responsible for poor patient outcome. The aim of this study is to develop a clinically applicable, non-invasive method to obtain a quantitative TAM detection in tumour tissue. The method is based on longitudinal proton relaxation rate (R(1)) measurements at low field (0.01-1 MHz) to assess the localization of ferumoxytol (clinical approved iron oxide particles) in TAM present in melanoma tumours, where R(1) = 1/T(1). R(1) at low magnetic fields appears highly dependent on the intra or extra cellular localization of the nanoparticles thus allowing an unambiguous TAM quantification. R(1) profiles were acquired on a Fast Field-Cycling relaxometer equipped with a 40 mm wide bore magnet and an 11 mm solenoid detection coil placed around the anatomical region of interest. The R(1) values measured 3 h and 24 h after the injection were significantly different. At 24 h R(1) exhibited a behavior similar to "in vitro" ferumoxytol-labelled J774A.1 macrophages whereas at 3 h, when the ferumoxytol distribution was extracellular, R(1) exhibited higher values similar to that of free ferumoxytol in solution. This finding clearly indicated the intracellular localization of ferumoxytol at 24 h, as confirmed by histological analysis (Pearls and CD68 assays). This information could be hardly achievable from measurements at a single magnetic field and opens new horizons for cell tracking applications using FFC-MRI.
In vivo assessment of tumour associated macrophages in murine melanoma obtained by low-field relaxometry in the presence of iron oxide particles
No results. Nessun risultato.
In vivo assessment of tumour associated macrophages in murine melanoma obtained by low-field relaxometry in the presence of iron oxide particles
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Hesse B, Tagil K, Cuocolo A, Anagnostopoulos C, Bardies M, Bax J, Bengel F, Busemann Sokole E, Davies G, Dondi M, Edenbrandt L, Franken P, Kjaer A, Knuuti J, Lassmann M, Ljungberg M, Marcassa C, Marie PY, Mckiddie F, O'connor M, Prvuolovich E, Underwood R * 3. 0 T perfusion MR imaging(551 visite) Rivista Di Neuroradiologia (ISSN: 1120-9976), 2004; 17(6): 807-812. Impact Factor:0.023 DettagliEsporta in BibTeXEsporta in EndNote
354 Records (334 escludendo Abstract e Conferenze). Impact factor totale: 1140.806 (1096.773 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 1203.977 (1155.905 escludendo Abstract e Conferenze).
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