Combined effects of probiotic and chondroprotector during osteoarthritis in rats (26 views visite)
Panminerva Med (ISSN: 0031-0808linking), 2020 Jun; 62(2): 93-101.
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Paper type Tipo di articolo: Journal Article
Impact factor: 3.467, 5-year impact factor Impact factor a 5 anni: 1.488
Url: Not available. Non disponibile.
Keywords Parole chiave: Not available. Non disponibili.
Affiliations Affiliazioni:
*** IBB - CNR ***
Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Laboratory of Pathology, CSD Health Care, Kyiv, Ukraine.
Department of Endocrinology, Bogomolets National Medical University, Kyiv, Ukraine.
Department of Health Sciences, Magna Graecia University, Catanzaro, Italy.
Institute of Biostructures and Bioimaging, Molecular Biotechnology Center, National Research Council (CNR), Turin, Italy.
Unit of Gastroenterology, Molinette Hospital, Turin, Italy.
References Riferimenti:
Not available. Non disponibili.
Panminerva Med (ISSN: 0031-0808linking), 2020 Jun; 62(2): 93-101.
Export to Esporta in BibTeX Export to Esporta in EndNote
Paper type Tipo di articolo: Journal Article
Impact factor: 3.467, 5-year impact factor Impact factor a 5 anni: 1.488
Url: Not available. Non disponibile.
Keywords Parole chiave: Not available. Non disponibili.
Affiliations Affiliazioni:
*** IBB - CNR ***
Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Laboratory of Pathology, CSD Health Care, Kyiv, Ukraine.
Department of Endocrinology, Bogomolets National Medical University, Kyiv, Ukraine.
Department of Health Sciences, Magna Graecia University, Catanzaro, Italy.
Institute of Biostructures and Bioimaging, Molecular Biotechnology Center, National Research Council (CNR), Turin, Italy.
Unit of Gastroenterology, Molinette Hospital, Turin, Italy.
References Riferimenti:
Not available. Non disponibili.
Combined effects of probiotic and chondroprotector during osteoarthritis in rats
BACKGROUND: Osteoarthritis (OA) is a joint affection, defined by articular cartilage demolition, risks of which rise with age. The aim of this study was to compare the efficacy of chondroitin sulfate (CS) course and multistrain live probiotic (LP) administered alone or in combination on the expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane during OA in rats. METHODS: OA was induced in male rats by injecting monoiodoacetate (MIA) in right hind knee. Therapeutic groups received 3 mg/kg of chondroprotector (ChP) CS for 28 days and/or 140 mg/kg of LP diet for 14 days. The expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane were determined with immunohistochemical staining kits (Thermo Fisher Scientific). RESULTS: It was established that MIA injection is associated with long-term structural changes in joint tissues that corresponded to OA-like features and associated with activation of pathogen-recognizing molecules and proinflammatory signaling pathways expression. Separate therapy with ChP and probiotics slightly decreased OA score limiting cell death and subchondral bone resorption. However, these changes were not associated with a significant decrease in TLR-2, TLR-4, NF-kB and TNF-α expression. On the other hand, the combination of ChP and LP treatment significantly decreased OA score. This correlated with a decrease in TLR-2, TLR-4, NF-kB and TNF-α expression in chondrocytes and synovial cells. CONCLUSIONS: The outcomes of our research prove that ChPs amplify the positive action of LPs in OA attenuation.
BACKGROUND: Osteoarthritis (OA) is a joint affection, defined by articular cartilage demolition, risks of which rise with age. The aim of this study was to compare the efficacy of chondroitin sulfate (CS) course and multistrain live probiotic (LP) administered alone or in combination on the expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane during OA in rats. METHODS: OA was induced in male rats by injecting monoiodoacetate (MIA) in right hind knee. Therapeutic groups received 3 mg/kg of chondroprotector (ChP) CS for 28 days and/or 140 mg/kg of LP diet for 14 days. The expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane were determined with immunohistochemical staining kits (Thermo Fisher Scientific). RESULTS: It was established that MIA injection is associated with long-term structural changes in joint tissues that corresponded to OA-like features and associated with activation of pathogen-recognizing molecules and proinflammatory signaling pathways expression. Separate therapy with ChP and probiotics slightly decreased OA score limiting cell death and subchondral bone resorption. However, these changes were not associated with a significant decrease in TLR-2, TLR-4, NF-kB and TNF-α expression. On the other hand, the combination of ChP and LP treatment significantly decreased OA score. This correlated with a decrease in TLR-2, TLR-4, NF-kB and TNF-α expression in chondrocytes and synovial cells. CONCLUSIONS: The outcomes of our research prove that ChPs amplify the positive action of LPs in OA attenuation.
Combined effects of probiotic and chondroprotector during osteoarthritis in rats
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Combined effects of probiotic and chondroprotector during osteoarthritis in rats
Korotkyi OH, Vovk AA, Dranitsina AS, Falalyeyeva TM, Dvorshchenko KO, Fagoonee S, Ostapchenko LI
* The influence of probiotic diet and chondroitin sulfate administration on Ptgs2, Tgfb1 and Col2a1 expression in rat knee cartilage during monoiodoacetate-induced osteoarthritis (230 visite)
Hepatology (ISSN: 0026-4806linking, 1827-1669electronic), 2019 Apr 1; 110(5): 419-424.
Impact Factor: 2.863
Dettagli Esporta in BibTeX Esporta in EndNote
Rosa D, Balato G, Ciaramella G, Soscia E, Improta G, Triassi M
* Long-term clinical results and MRI changes after autologous chondrocyte implantation in the knee of young and active middle aged patients (372 visite)
J Orthop Traumatol (ISSN: 1590-9999electronic, 1590-9921linking), 2015 Oct 24; 17(1): 55-62.
Impact Factor: 1.28
Dettagli Esporta in BibTeX Esporta in EndNote
Soscia E, Sirignano C, Catalano O, Atteno M, Costa L, Caso F, Peluso R, Bruner V, Aquino MM, Del Puente A, Salvatore M, Scarpa R
* New developments in magnetic resonance imaging of the nail unit (286 visite)
Journal Of Rheumatology (ISSN: 0315-162x), 2012 Jul; 39(SUPPL.89): 49-53.
Impact Factor: 3.258
Dettagli Esporta in BibTeX Esporta in EndNote
3 Records (3 escludendo Abstract e Conferenze).
Impact factor totale: 7.401 (7.401 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 5.059 (5.059 escludendo Abstract e Conferenze).
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Korotkyi OH, Vovk AA, Dranitsina AS, Falalyeyeva TM, Dvorshchenko KO, Fagoonee S, Ostapchenko LI
* The influence of probiotic diet and chondroitin sulfate administration on Ptgs2, Tgfb1 and Col2a1 expression in rat knee cartilage during monoiodoacetate-induced osteoarthritis (230 visite)
Hepatology (ISSN: 0026-4806linking, 1827-1669electronic), 2019 Apr 1; 110(5): 419-424.
Impact Factor: 2.863
Dettagli Esporta in BibTeX Esporta in EndNote
Rosa D, Balato G, Ciaramella G, Soscia E, Improta G, Triassi M
* Long-term clinical results and MRI changes after autologous chondrocyte implantation in the knee of young and active middle aged patients (372 visite)
J Orthop Traumatol (ISSN: 1590-9999electronic, 1590-9921linking), 2015 Oct 24; 17(1): 55-62.
Impact Factor: 1.28
Dettagli Esporta in BibTeX Esporta in EndNote
Soscia E, Sirignano C, Catalano O, Atteno M, Costa L, Caso F, Peluso R, Bruner V, Aquino MM, Del Puente A, Salvatore M, Scarpa R
* New developments in magnetic resonance imaging of the nail unit (286 visite)
Journal Of Rheumatology (ISSN: 0315-162x), 2012 Jul; 39(SUPPL.89): 49-53.
Impact Factor: 3.258
Dettagli Esporta in BibTeX Esporta in EndNote
3 Records (3 escludendo Abstract e Conferenze).
Impact factor totale: 7.401 (7.401 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 5.059 (5.059 escludendo Abstract e Conferenze).
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