Understanding the binding properties of an unusual metal-binding protein - a study of bacterial frataxin(234 views visite) Pastore C, Franzese M, Sica F, Temussi P, Pastore A
Keywords Parole chiave: Cyay, Friedreich, S Ataxia, Iron Binding, X-Ray, Aspartic Acid, Cation, Cyay Protein, Cysteine, Frataxin, Glutamic Acid, Histidine, Iron Binding Protein, Metal, Unclassified Drug, Binding Affinity, Binding Site, Electricity, Iron Chelation, Metal Binding, Nuclear Magnetic Resonance, Orthology, Priority Journal, Protein Binding, Protein Function, X Ray Crystallography, Amino Acid Sequence, Bacterial Proteins, Competitive, Cobalt, Gadolinium, Iron-Binding Proteins, Iron-Sulfur Proteins, Lanthanoid Series Elements, Lutetium, Magnesium, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Mutation, Protein Conformation, Protein Structure, Tertiary, Sequence Homology, Zinc, Bacteria (microorganisms),
Affiliations Affiliazioni: *** IBB - CNR ***
NIMR, The Ridgeway, London, NW71AA, United Kingdom National Institute for Medical Research, London, United Kingdom Dipartimento di Chimica, University of Naples, Italy Istituto di Biostrutture e Bioimmagini, CNR, Naples, Italy
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Bulteau, A. L., O'Neill, H. A., Kennedy, M. C., Ikeda-Saito, M., Isaya, G., Szweda, L. I., Frataxin acts as an iron chaperone protein to modulate mitochondrial aconitase activity (2004) Science, 305, pp. 242-245
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Radisky, D. C., Babcock, M. C., Kaplan, J., The yeast frataxin homologue mediates mitochondrial iron efflux: Evidence for a mitochondrial iron cycle (1999) J Biol Chem, 274, pp. 4497-4499
Que Jr., L., Ho, R. Y., Dioxygen activation by enzymes with mononuclear non-heme iron active sites (1996) Chem Rev, 96, pp. 2607-2624
Cho, S. J., Lee, M. G., Yang, J. Y., Song, H. K., Suh, S. W., Crystal structure of Escherichia coli CyaY reveals a previously unidentified fold for the evolutionary conserved frataxin family (2000) Proc Natl Acad Sci USA, 97, pp. 8932-8937
He, Y., Alam, S. L., Proteasa, S. V., Zhang, Y., Lesuisse, E., Dancis, A., Stemmler, T. L., Yeast frataxin solution structure, iron binding and ferrochelatase interaction (2004) Biochemistry, 43, pp. 16254-16262
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Carter Jr., C. W., Sweet, R. M., Macromolecular crystallography. Part a (1997) Methods Enzymol, 276, pp. 530-538
Br nger, A. T., Adams, P. D., Clore, G. M., Delano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. -S., Pannu, N. S., Crystallography and NMR system: A new software suite for macromolecular structure determination (1998) Acta Crystallogr, 54, pp. 905-921
Schneider, T. R., Sheldrick, G. M., Substructure solution with SHELXD (2002) Acta Crystallogr D Biol Crystallogr, 58, pp. 1772-1779
Jones, T. A., Zou, J. Y., Cowan, S. W., Kjeldgaard, M., Improved methods for building protein models in electron density maps and the location of errors in these models (1991) Acta Crystallogr, 47, pp. 110-119
Laskowski, R. A., MacArthur, M. W., Moss, D. S., Thornton, J. M., PROCHECK: A program to check the stereochemical quality of protein structure (1993) J Appl Crystallogr, 26, pp. 283-291
Hooft, R. W., Vriend, G. S., Abola, E. E., Errors in protein structures (1996) Nature, 381, p. 272
Esnouf, R. M., Further additions to Molscript, Version 1. 4, including reading and contouring of electron-density maps (1999) Acta Crystallogr, 55, pp. 938-940
Understanding the binding properties of an unusual metal-binding protein - a study of bacterial frataxin
Deficiency of the small mitochondrial protein frataxin causes Friedreich's ataxia, a severe neurodegenerative pathology. Frataxin, which has been highly conserved throughout evolution, is thought to be involved in, among other processes, Fe-S cluster formation. Independent evidence shows that it binds iron directly, although with very distinct features and low affinity. Here, we have carried out an extensive study of the binding properties of CyaY, the bacterial ortholog of frataxin, to different divalent and trivalent cations, using NMR and X-ray crystallography. We demonstrate that the protein has low cation specificity and contains multiple binding sites able to chelate divalent and trivalent metals with low affinity. Binding does not involve cavities or pockets, but exposed glutamates and aspartates, which are residues that are unusual for iron chelation when not assisted by histidines and/or cysteines. We have related how such an ability to bind cations on a relatively large area through an electrostatic mechanism could be a valuable asset for protein function.
Understanding the binding properties of an unusual metal-binding protein - a study of bacterial frataxin
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ * Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer(204 visite) N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756. Impact Factor:59.558 DettagliEsporta in BibTeXEsporta in EndNote
Malvindi MA, Greco A, Conversano F, Figuerola A, Corti M, Bonora M, Lascialfari A, Doumari HA, Moscardini M, Cingolani R, Gigli G, Casciaro S, Pellegrino T, Ragusa A * MR Contrast Agents(217 visite) Small Animal Imaging, 2011 Jul 8; 21(13): 2548-2555. Impact Factor:1.784 DettagliEsporta in BibTeXEsporta in EndNote
Testino G, Leone S, Fagoonee S, Del Bas JM, Rodriguez B, Puiggros F, Marine S, Rodriguez MA, Morina D, Armengol L, Caimari A, Arola L, Cimini FA, Barchetta I, Carotti S, Bertoccini L, Baroni MG, Vespasiani-gentilucci U, Cavallo MG, Morini S, Nelson JE, Roth CL, Wilson LA, Yates KP, Aouizerat B, Morgan-stevenson V, Whalen E, Hoofnagle A, Mason M, Gersuk V, Yeh MM, Kowdley KV, Lee SM, Jun DW, Cho YK, Jang KS, Kucukazman M, Ata N, Dal K, Yeniova AO, Kefeli A, Basyigit S, Aktas B, Akin KO, Agladioglu K, Ure OS, Topal F, Nazligul Y, Beyan E, Ertugrul DT, Catena C, Cosma C, Camozzi V, Plebani M, Ermani M, Sechi LA, Fallo F, Goto Y, Ray MB, Mendenhall CL, French SW, Gartside PS Serum vitamin A deficiency and increased intrahepatic expression of cytokeratin antigen in alcoholic liver disease(418 visite) Hepatology (ISSN: 1827-1669electronic, 0026-4806linking), 1988 Sep; 83120693611123109(5): 1019-1026. Impact Factor:0.913 DettagliEsporta in BibTeXEsporta in EndNote
653 Records (608 escludendo Abstract e Conferenze). Impact factor totale: 2320.634 (2170.431 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 2287.843 (2121.848 escludendo Abstract e Conferenze).
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