Transcription factor decoy molecules based on a peptide nucleic acid (PNA)-DNA chimera mimicking Sp1 binding sites(320 views visite) Borgatti M, Lampronti I, Romanelli A, Pedone C, Saviano M, Bianchi N, Mischiati C, Gambari R
Keywords Parole chiave: Biochemistry, Molecular Biology, Proteins, Peptides, Nucleic Acids, Cation, Chimeric Protein, Dna Hybrid, Double Stranded Dna, Glycine Derivative, Liposome, N (2 Aminoethyl)glycine, Nuclear Protein, Peptide Nucleic Acid, Pseudopeptide, Transcription Factor Sp1, Unclassified Drug, Hybrid Protein, Oligonucleotide, Article, Binding Affinity, Binding Site, Cell Membrane Resistance, Chemical Structure, Complex Formation, Controlled Study, Dna Dna Hybridization, Dna Strand, Enzyme Degradation, Gene Therapy, Human, Human Cell, Molecular Interaction, Molecular Mimicry, Molecular Stability, Priority Journal, Protein Dna Binding, Protein Structure, Transcription Regulation, Cell Nucleus, Cell Strain K 562, Genetic Transfection, Metabolism, Protein Binding, Protein Tertiary Structure, K562 Cells, Recombinant Fusion Proteins, Sp1 Transcription Factor, Time Factors, N (2 Aminoethyl) Glycine,
Affiliations Affiliazioni: *** IBB - CNR ***
Dept. of Biochem./Molecular Biology, Ferrara University, Via L. Borsari n.46, 44100 Ferrara, Italy Inst. of Biostructure and Bioimaging, CNR, 80134 Napoli, Italy Biotechnology Centre, Ferrara University, Via Fossato di Mortara 8/A, 44100 Ferrara, Italy Dept. of Biochem. /Molecular Biology, Ferrara University, Via L. Borsari n. 46, 44100 Ferrara, Italy
References Riferimenti: Clusel, C., Meguenni, S., Elias, I., Vasseur, M., Blumenfeld, M., (1994) Gene Expr., 4, pp. 301-30
Cho-Chung, Y.S., Park, Y.G., Nesterova, M., Lee, Y.N., Cho, Y.S., (2000) Mol. Cell. Biochem., 212, pp. 29-34
Ishibashi, H., Nakagawa, K., Onimaru, M., Castellanous, E.J., Kaneda, Y., Nakashima, Y., Shirasuna, K., Sueishi, K., (2000) Cancer Res., 60, pp. 6531-6536
Kawauchi, M., Suzuki, J., Morishita, R., Wada, Y., Izawa, A., Tomita, N., Amano, J., Isobe, M., (2000) Circ. Res., 87, pp. 1063-1068
Lee, Y.N., Park, Y.G., Choi, Y.H., Cho, Y.S., Cho-Chung, Y.S., (2000) Biochemistry, 39, pp. 4863-4868
Tomita, N., Morishita, R., Tomita, S., Gibbons, G.H., Zhang, L., Horiuchi, M., Kaneda, Y., Dzau, V.J., (2000) Gene Ther., 7, pp. 1326-1332
Vos, I.H., Govers, R., Grone, H.J., Kleij, L., Schurink, M., De Weger, R.A., Goldschmeding, R., Rabelink, T.J., (2000) FASEB J., 14, pp. 815-822
Kozlov, I.A., Kubareva, E.A., Ivanovskaya, M.G., Shabarova, Z.A., (1997) Antisense Nucleic Acid Drug Dev., 7, pp. 279-289
Iwase, R., Namba, M., Yamaoka, T., Murakami, A., (1997) Nucleic Acids Symp. Ser., 37, pp. 203-204
Yamashita, J., Yoshimasa, T., Arai, H., Hiraoka, J., Tayaya, K., Miyamoto, Y., Ogawa, Y., Nakao, K., (1998) J. Biol. Chem., 273, pp. 15993-15999
Morishita, R., Gibbons, G.H., Horiuchi, M., Ellison, K.E., Nakama, M., Zhang, L., Kaneda, Y., Dzau, V.J., (1995) Proc. Natl. Acad. Sci. U. S. A., 92, pp. 5855-5859
Morishita, R., Sugimoto, T., Aoki, M., Kida, I., Tomita, N., Moriguchi, A., Maeda, K., Ogihara, T., (1997) Nat. Med., 3, pp. 894-899
Ono, S., Date, I., Onoda, K., Shiota, T., Ohmoto, T., Ninomiya, Y., Asari, S., Morishita, R., (1998) Hum. Gene Ther., 9, pp. 1003-1011
Sharma, H.W., Perez, J.R., Higgins-Sochaski, K., Hsiao, R., Narayanan, R., (1996) Anticancer Res., 16, pp. 61-69
Hata, Y., Duh, E., Zhang, K., Robinson, G.S., Aiello, L.P., (1998) J. Biol. Chem., 273, pp. 19294-19303
Gaynor, R., (1992) AIDS, 6, pp. 347-363
Nielsen, P.E., Egholm, M., Berg, R.H., Buchardt, O., (1991) Science, 254, pp. 1497-1500
Egholm, M., Buchardt, O., Nielsen, P.E., Berg, R.H., (1992) J. Am. Chem. Soc., 114, pp. 1895-1897
Gambari, R., (2001) Curr. Pharm. Des., 7, pp. 1839-1862
Saviano, M., Romanelli, A., Bucci, E., Pedone, C., Mischiati, C., Bianchi, N., Feriotto, G., Gambari, R., (2000) J. Biomol. Struct. Dyn., 18, pp. 353-362
Demidov, V.V., Potaman, V.N., Frank-Kamenetsk, M.D., Egholm, M., Buchard, O., Sonnichsen, S.H., Nielsen, P.E., (1994) Biochem. Pharmacol., 48, pp. 1310-1313
Bianchi, N., Osti, F., Rutigliano, C., Ginanni Corradini, F., Borsetti, E., Tomassetti, M., Mischiati, C., Gambari, R., (1999) Br. J. Haematol., 104, pp. 258-265
Bianchi, N., Chiarabelli, C., Borgatti, M., Mischiati, C., Fibach, E., Gambari, R., (2001) Br. J. Haematol., 113, pp. 951-961
Gambari, R., Del Senno, L., Barbieri, R., Viola, L., Tripodi, M., Raschellà, G., Fantoni, A., (1984) Cell Differ., 14, pp. 87-97
Bianchi, N., Ongaro, F., Chiarabelli, C., Gualandi, L., Mischiati, C., Bergamini, P., Gambari, R., (2000) Biochem. Pharmacol., 60, pp. 31-40
Nastruzzi, C., Cortesi, R., Esposito, E., Gambari, R., Borgatti, M., Bianchi, N., Feriotto, G., Mischiati, C., (2000) J. Controlled Release, 68, pp. 237-249
Nastruzzi, C., Gambari, R., Menegatti, E., Walde, P., Luisi, P.L., (1990) J. Pharm. Sci., 79, pp. 672-677
Goodwin, A.J., McInerney, J.M., Glander, M.A., Pomerantz, O., Lowrey, C.H., (2001) J. Biol. Chem., 276, pp. 26883-26892
Gregory, R.C., Taxman, D.J., Seshasayee, D., Kensinger, M.H., Bieker, J.J., Wojchowski, D.M., (1996) Blood, 87, pp. 1793-1801
Pondel, M.D., Murphy, S., Pearson, L., Craddock, C., Proudfoot, N.J., (1995) Proc. Natl. Acad. Sci. U. S. A., 92, pp. 7237-7241
Merika, M., Orkin, S.H., (1995) Mol. Cell. Biol., 15, pp. 2437-2447
Bacon, E.R., Dalyot, N., Filon, D., Schreiber, L., Rachmilewitz, E.A., Oppenheim, A., (1995) Mol. Med., 1, pp. 297-305
Romanelli, E., Pedone, C., Saviano, M., Bianchi, N., Borgatti, M., Mischiati, C., Gambari, R., (2001) Eur. J. Biochem., 268, pp. 1-11
Borgatti, M., Breda, L., Cortesi, R., Nastruzzi, C., Romanelli, A., Saviano, M., Bianchi, N., Gambari, R., (2002) Biochem. Pharmacol., 64, pp. 609-616
Transcription factor decoy molecules based on a peptide nucleic acid (PNA)-DNA chimera mimicking Sp1 binding sites
Peptide nucleic acids (PNAs) are DNA-mimicking molecules in which the sugar-phosphate backbone is replaced by a pseudopeptide backbone composed of N-(2aminoethyl)glycine units. We determined whether double-stranded molecules based on PNAs and PNA-DNA-PNA (PDP) chimeras could be capable of stable interactions with nuclear proteins belonging to the Sp1 transcription factor family and, therefore, could act as decoy reagents able to inhibit molecular interactions between Sp1 and DNA. Since the structure of PNA/PNA hybrids is very different from that of the DNA/DNA double helix, they could theoretically alter the molecular structure of the double-stranded PNA-DNA-PNA chimeras, perturbing interactions with specific transcription factors. We found that PNA-based hybrids do not inhibit Sp1/DNA interactions. In contrast, hybrid molecules based on PNA-DNA-PNA chimeras are very effective decoy molecules, encouraging further experiments focused on the possible use of these molecules for the development of potential agents for a decoy approach in gene therapy. In this respect, the finding that PDP-based decoy molecules are more resistant than DNA/DNA hybrids to enzymatic degradation appears to be of great interest. Furthermore, their resistance can even be improved after complexation with cationic liposomes to which PDP/PDP chimeras are able to bind by virtue of their internal DNA structure.
Transcription factor decoy molecules based on a peptide nucleic acid (PNA)-DNA chimera mimicking Sp1 binding sites
Chattopadhyay A, Cogdell R, Crespo-hernandez CE, Datta A, De A, Haacke S, Hariharan M, Helliwell J, Hughes A, Improta R, Jones M, Joseph J, Karsili T, Kohler B, Krishnan R, Kuriakose A, L , M , Markovitsi D, Medhi H, Periyasamy G, Pradeepkumar PI, Roy Chowdhury P, Sarangi M, Schapiro I, Schertler GFX, Schlichting I, Segarra-marti J, Swaminathan R, V , V , Van Grondelle R, Venkatraman RK, Venkatramani R, Watts A * Light induced charge and energy transport in nucleic acids and proteins: general discussion(121 visite) Faraday Discuss (ISSN: 1359-6640print, 1359-6640linking), 2018 Apr 1; 207: 153-180. Impact Factor:4.194 DettagliEsporta in BibTeXEsporta in EndNote
Chandra A, Cogdell R, Crespo-hernandez CE, Datta A, Giussani A, Haacke S, Helliwell J, Improta R, Jayasree RS, Jones M, Karsili T, Kohler B, L , M , Mandal I, Markovitsi D, Medhi H, Mishra PP, Pradeepkumar PI, Roy Chowdhury P, Sarangi M, Schapiro I, Schlichting I, Segarra-marti J, Sharma A, V , V , Van Grondelle R, Watts A * Light induced damage and repair in nucleic acids and proteins: general discussion(102 visite) Faraday Discuss (ISSN: 1359-6640print, 1359-6640linking), 2018 Apr 1; 207: 389-408. Impact Factor:4.194 DettagliEsporta in BibTeXEsporta in EndNote
Bhat V, Cogdell R, Crespo-hernández CE, Datta A, De A, Haacke S, Helliwell J, Improta R, Joseph J, Karsili T, Kohler B, Krishnan R, Mahil LM, Lewis F, Mandal I, Markovitsi D, Mishra PP, Paul S, Periyasamy G, Pradeepkumar PI, Roy Chowdhury P, Sarangi M, Sasikumar D, Schapiro I, Schertler GFX, Schlichting I, Segarra-martí J, Swaminathan R, Vishnu V, Van Grondelle R, Varghese R, Venkatramani R * Photocrosslinking between nucleic acids and proteins: general discussion(110 visite) Faraday Discussions, 2018; N/D: N/D-N/D. Impact Factor:3.427 DettagliEsporta in BibTeXEsporta in EndNote
Kim YH, Shin SW, Pellicano R, Fagoonee S, Choi IJ, Kim YI, Park B, Choi JM, Kim SG, Choi J, Park JY, Oh S, Yang HJ, Lim JH, Im JP, Kim JS, Jung HC, Ponzetto A, Figura N, Malfertheiner P, Choi IJ, Kook MC, Kim YI, Cho SJ, Lee JY, Kim CG, Park B, Nam BH, Bae SE, Choi KD, Choe J, Kim SO, Na HK, Choi JY, Ahn JY, Jung KW, Lee J, Kim DH, Chang HS, Song HJ, Lee GH, Jung HY, Seta T, Takahashi Y, Noguchi Y, Shikata S, Sakai T, Sakai K, Yamashita Y, Nakayama T, Leja M, Park JY, Murillo R, Liepniece-karele I, Isajevs S, Kikuste I, Rudzite D, Krike P, Parshutin S, Polaka I, Kirsners A, Santare D, Folkmanis V, Daugule I, Plummer M, Herrero R, Tsukamoto T, Nakagawa M, Kiriyama Y, Toyoda T, Cao X, Corral JE, Mera R, Dye CW, Morgan DR, Lee YC, Lin JT, Garcia Martin R, Matia Cubillo A, Lee SH, Park JM, Han YM, Ko WJ, Hahm KB, Leontiadis GI, Ford AC, Ichinose M, Sugano K, Jeong M, Park JM, Han YM, Park KY, Lee DH, Yoo JH, Cho JY, Hahm KB, Bang CS, Baik GH, Shin IS, Kim JB, Suk KT, Yoon JH, Kim YS, Kim DJ * Helicobacter pylori Eradication for Prevention of Metachronous Recurrence after Endoscopic Resection of Early Gastric Cancer(217 visite) N Engl J Med (ISSN: 0028-4793, 0028-4793linking, 1533-4406electronic), 2015 Jun; 30642104201566393291: 749-756. Impact Factor:59.558 DettagliEsporta in BibTeXEsporta in EndNote
Antonini A, Vitale C, Barone P, Cilia R, Righini A, Bonuccelli U, Abbruzzese G, Ramat S, Petrone A, Quatrale R, Marconi R, Ceravolo R, Stefani A, Lopiano L, Zappia M, Capus L, Morgante L, Tamma F, Tinazzi M, Colosimo C, Guerra UP, Valzania F, Fagioli G, Distefano A, Bagnato A, Feggi L, Anna S, Maria Teresa Rosaria De Cr, Nobili F, Mazzuca N, Baldari S, Eleopra R, Bestetti A, Benti R, Varrone A, Volterrani D, Massa R, Stocchi F, Schillaci O, Dore F, Zibetti M, Castellano G, Battista SG, Giorgetti G * The relationship between cerebral vascular disease and parkinsonism: The VADO study(422 visite) Parkinsonism Relat D (ISSN: 1353-8020, 1873-5126, 1873-5126electronic), 2012; 18(6): 775-780. Impact Factor:3.274 DettagliEsporta in BibTeXEsporta in EndNote
Malvindi MA, Greco A, Conversano F, Figuerola A, Corti M, Bonora M, Lascialfari A, Doumari HA, Moscardini M, Cingolani R, Gigli G, Casciaro S, Pellegrino T, Ragusa A * MR Contrast Agents(230 visite) Small Animal Imaging, 2011 Jul 8; 21(13): 2548-2555. Impact Factor:1.784 DettagliEsporta in BibTeXEsporta in EndNote