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Chemical basis of peptidoglycan discrimination by PrkC, a key kinase involved in bacterial resuscitation from dormancy (95 visite)

Squeglia F, Marchetti R, Ruggiero A, Lanzetta R, Marasco D, Dworkin J, Petoukhov M, Molinaro A, Berisio R, Silipo A

J Am Chem Soc (ISSN: 0002-7863, 0002-2786, 1520-5126), 2011 Dec 28; 133(51): 20676-20679.



Tipo di articolo: Journal Article,

Impact factor: 9.907, Impact factor a 5 anni: 9.766

Url: http://www.scopus.com/inward/record.url?eid=2-s2.0-84555178075&partnerID=40&md5=1385a115e0ceea89e17d6e3defc8f053

Parole chiave: Arginine Residue, Bacillus Subtilis, Bacterial Spore, Cell Walls, Cellular Process, Extracellular Region, Growing Conditions, Molecular Signals, Muropeptides, Nmr Techniques, Peptidoglycans, Protein Mutagenesis, Structural Requirements, Bacteriology, Resuscitation, Walls (structural Partitions), Enzymes, Amino Acid Derivative, Bacterial Enzyme, Diaminopimelic Acid, Prkc Protein, Protein Serine Threonine Kinase, Unclassified Drug, Article, Bacterial Cell Wall, Bacterial Growth, Bacterial Survival, Binding Site, Carbohydrate Analysis, Carboxy Terminal Sequence, Cell Growth, Circular Dichroism, Controlled Study, Dormancy, Enzyme Binding, Extracellular Space, Germination, In Vitro Study, Nonhuman, Nuclear Magnetic Resonance Spectroscopy, Protein Domain, Protein Interaction, Protein Structure, Signal Transduction, Staphylococcus Aureus, Models, Mutation, Biomolecular, Protein Binding, Protein Conformation, Tertiary, Protein-Serine-Threonine Kinases, Substrate Specificity,

Affiliazioni:

*** IBB - CNR ***
Institute of Biostructures and Bioimaging, Consiglio Nazionale Delle Ricerche (CNR), Via Mezzocannone 16, I-80134 Napoli, Italy
Department of Organic and Biological Chemistry, University of Naples Federico II, Via Cinthia 4, I-80126 Napoli, Italy
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, United States
European Molecular Biology Laboratory, Hamburg Outstation, C/o DESY, Notkestrasse 85, 22607 Hamburg, Germany



Riferimenti:

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Ruggiero, A., Tizzano, B., Pedone, E., Pedone, C., Wilmanns, M., Berisio, R., (2009) J. Mol. Biol., 385, p. 153

Ruggiero, A., Marasco, D., Squeglia, F., Soldini, S., Pedone, E., Pedone, C., Berisio, R., (2010) Structure, 18, p. 1184

Kaprelyants, A.S., Mukamolova, G.V., Ruggiero, A., Makarov, V.A., Demina, G.R., Shleeva, M.O., Potapov, V.D., Shramko, P., (2011) Protein Pept. Lett., , not supplied

Shah, I.M., Laaberki, M.H., Popham, D.L., Dworkin, J., (2008) Cell, 135, p. 486

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Ruggiero, A., Squeglia, F., Marasco, D., Marchetti, R., Molinaro, A., Berisio, R., (2011) Biochem. J., 435, p. 33

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Hendrickx, A. P., Budzik, J. M., Oh, S. Y., Schneewind, O., (2011) Nat. Rev. Microbiol., 9, p. 16

Kaprelyants, A. S., Mukamolova, G. V., Ruggiero, A., Makarov, V. A., Demina, G. R., Shleeva, M. O., Potapov, V. D., Shramko, P., (2011) Protein Pept. Lett., , not supplied

Shah, I. M., Laaberki, M. H., Popham, D. L., Dworkin, J., (2008) Cell, 135, p. 486

Lim, J. H., Kim, M. S., Kim, H. E., Yano, T., Oshima, Y., Aggarwal, K., Goldman, W. E., Oh, B. H., (2006) J. Biol. Chem., 281, p. 8286



Bacterial Ser/Thr kinases modulate a wide number of cellular processes. In Bacillus subtilis, the Ser/Thr kinase PrkC has been shown to induce germination of bacterial spores in response to DAP-type but not Lys-type cell wall muropeptides. Muropeptides are a clear molecular signal that growing conditions are promising, since they are produced during cell wall peptidoglycan remodeling associated with cell growth and division of neighboring bacteria. However, whether muropeptides are able to bind the protein physically and how the extracellular region is able to distinguish the two types of muropeptides remains unclear. Here we tackled the important question of how the extracellular region of PrkC (EC-PrkC) senses muropeptides. By coupling NMR techniques and protein mutagenesis, we exploited the structural requirements necessary for recognition and binding and proved that muropeptides physically bind to EC-PrkC through DAP-moiety-mediated interactions with an arginine residue, Arg500, belonging to the protein C-terminal PASTA domain. Notably, mutation of this arginine completely suppresses muropeptide binding. Our data provide the first molecular clues into the mechanism of sensing of muropeptides by PrkC. © 2011 American Chemical Society.
Nessun risultato.
Nessun risultato.

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L , Q , Fabbri G, Patacchini R, A , Maggi C, Astolfi M, D'Auria G, Maglio O, Lombardi A, Pedone C, Pavone V
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30 Records (30 escludendo Abstract e Conferenze).
Impact factor totale: 91.888 (91.888 escludendo Abstract e Conferenze).
Impact factor a 5 anni totale: 86.591 (86.591 escludendo Abstract e Conferenze).







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