A protein-based biointerfacing route toward label-free immunoassays with long period gratings in transition mode(204 visite) Pilla P, Sandomenico A, Malachovsk V, Borriello A, Giordano M, Cutolo A, Ruvo M, Cusano A
Parole chiave: Biomolecular Sensing, Immobilization Technique, Long Period Gratings, Modal Transition, Polymer Waveguides, Adhesion, Aldehydes, Body Fluids, Film Thickness, Optimization, Polystyrenes, Refractive Index, Surface Plasmon Resonance, Surface Testing, Transition Flow, Fiber Optic Sensors, Bovine Serum Albumin, Glutaraldehyde, Immunoglobulin G, Immunoglobulin G Antibody, Antigen Antibody Reaction, Article, Biological Activity, Chemical Labeling, Controlled Study, Enzyme Linked Immunosorbent Assay, Equipment Design, Immunoassay, Material Coating, Process Monitoring, Protein Assembly, Protein Immobilization, Reaction Optimization, Reaction Time, Refraction Index, Sensitivity Analysis, Surface Property, Transducer, Biosensing Techniques, Equipment Failure Analysis, Humans, Protein Array Analysis, Refractometry, Reproducibility Of Results, Sensitivity And Specificity, Staining And Labeling,
*** IBB - CNR *** Optoelectronic Division, Engineering Department, University of Sannio, C.so Garibaldi 107, 82100 Benevento, Italy Institute of Biostructures and Bioimages, National Research Council, Via Mezzocannone 16, 80134 Napoli, Italy Institute of Composite and Biomedical Materials, National Research Council, P.le Tecchio 80, 80125 Napoli, Italy
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We present a fast and effective method for anchoring bioreceptors to optical waveguides exhibiting a poorly reactive polymer interface and that have to be minimally perturbed with respect to their design. The study originated from the need to biofunctionalize a fiber optic Long Period Grating (LPG) that is tuned in a highly sensitive working point, the so-called transition mode, through the deposition of a high refractive index overlay. In particular, a thin film of atactic polystyrene (PS) was dip-coated onto the LPG with a thickness suitable to optimize the LPG sensitivity to refractive index changes of the surrounding medium. Bovine serum albumin was selected as sacrificial layer for its well-known adhesion capabilities to PS surfaces, then glutaraldehyde was used to conjugate IgGs, serving as prototypical bioreceptor, on the device surface. The effectiveness of the immobilization method was assessed by studying the interaction between the immobilized IgG with a suitable anti-IgG. In a preliminary study performed by means of ELISA and surface plasmon resonance, optimal conditions for the biomolecular testing with the LPG were assessed. Four distinct interactions were thus monitored in real time following the shift of the LPG attenuation band. These experiments suggest a novel and interesting biofunctionalization approach of unreactive polymers with applications in immunosensing and basic life science research. (C) 2011 Elsevier BM. All rights reserved.
41 Records (40 escludendo Abstract e Conferenze). Impact factor totale: 142.05 (138.816 escludendo Abstract e Conferenze). Impact factor a 5 anni totale: 146.345 (142.643 escludendo Abstract e Conferenze).