Evidences Of Complex Formation Between Daba-Based Nucleo-Gamma-Peptides With Alternate Configuration Backbone(656 views) Roviello GN, Musumeci D, Moccia M, Castiglione M, Cesarani A, Bucci EM, Saviano M, Pedone C, Benedetti E
Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, Napoli 80134, Italy
Bionucleon Srl, via Montesano 49, Napoli 80131, Italy
via Ribes 5, Colleretto Giocosa, Torino 10010, Italy
Università di Napoli Federico II, Dip. Scienze Biologiche, Sez. Biostrutture, via Mezzocannone 16, Napoli 80134, Italy
Universit di Napoli Federico II, Dip. Scienze Biologiche, Sez. Biostrutture, via Mezzocannone 16, Napoli 80134, Italy
References: Not available.
Evidences Of Complex Formation Between Daba-Based Nucleo-Gamma-Peptides With Alternate Configuration Backbone
In the present work, we report the synthesis and the characterization of dab PNA hexamers with diaminobutyric acid backbone of D-or/and L-configuration. In particular, the four nucleo-amino acids we synthesized, D- and L-cliaminobutyryl adenines and D- and L-diaminobutyryl thymines, were used in various combinations to assemble the following oligomers: H-G- (t (L-dab)) (6) -K-NH2, HG- (t (D-dab)) (6) -K-NH2, H-G- (a (L-dab)) (6) -K-NH2, H-G- (t (L-dab) -t (D-dab)) (3) -K-NH2, H-G- (a (L-dab) -a (D-dab)) (3) -K-NH2, H-G- (a (L-dab) -t (D-dab)) (3) -K-NH2 By using CD and UV spectroscopies, we investigated the ability of complementary dab PNA strands to bind to each other. We found that binding occurs only between oligomers with backbone of alternate configuration [(t (L-dab) -t (D-dab)) (3) / (a (L-dab) -a (D-dab)) (3) and (a (L-dab) -t (D-dab)) (3) / (a (L-dab) -t (D-dab)) (3)] and implies cooperative hydrogen bonds and base stacking. Furthermore, interesting properties relative to the self-complementary oligomer (a (L-dab) -t (D-dab)) (3) forming palindromic complexes emerged from preliminary dynamic light-scattering experiments that suggested the formation of multimeric aggregates. These results, together with the high serum stability of the DABA-based oligomers, as shown by HPLC analysis, encourage us to further study dab PNAs as new self-recognizing blo-inspired polymers, to develop new nanomaterials in biotechnological and biomedical applications. Copyright (C) 2008 European Pepticle Society and John Wiley & Sons, Ltd
Evidences Of Complex Formation Between Daba-Based Nucleo-Gamma-Peptides With Alternate Configuration Backbone
Kállay C, Dávid A, Timári S, Nagy EM, Sanna D, Garribba E, Micera G, De Bona P, Pappalardo G, Rizzarelli E, Sóvágó I * Copper(II) complexes of rat amylin fragments(517 views) Dalton T (ISSN: 1477-9234, 1477-9226, 1477-9234electronic), 2011 Oct 14; 40(38): 9711-9721. Impact Factor:3.838 ViewExport to BibTeXExport to EndNote
Aloj L, Aurilio M, Rinaldi V, D'Ambrosio L, Tesauro D, Peitl PK, Maina T, Mansi R, Von Guggenberg E, Joosten L, Sosabowski JK, Breeman WA, De Blois E, Koelewijn S, Melis M, Waser B, Beetschen K, Reubi JC, De Jong M * The EEE project(543 views) Proc Int Cosm Ray Conf Icrc Universidad Nacional Autonoma De Mexico, 2007; 5(HEPART2): 977-980. Impact Factor:0 ViewExport to BibTeXExport to EndNote