Zinc to Cadmium Replacement in the A. thaliana SUPERMAN Cys(2)His(2) Zinc Finger Induces Structural Rearrangements of Typical DNA Base Determinant Positions
Zinc to Cadmium Replacement in the A. thaliana SUPERMAN Cys(2)His(2) Zinc Finger Induces Structural Rearrangements of Typical DNA Base Determinant Positions(572 views) Malgieri G, Zaccaro L, Leone M, Bucci E, Esposito S, Baglivo I, Del Gatto A, Russo L, Scandurra R, Pedone PV, Fattorusso R, Isernia C
Keywords: Arabidopsis Thaliana, Cadmium, Nmr Spectroscopy, Superman Protein, Zinc Finger Domain, Cadmium Ions, Carcinogenic Effects, Dissociation Constant, Dna Binding, Dna Recognition, Environmental Pollutions, High Mobility, High-Resolution Structures, Low Concentrations, Nmr Techniques, Relative Orientation, Secondary Structure Elements, Side-Chains, Structural Effect, Structural Rearrangement, Cadmium Compounds, Heavy Metals, Nuclear Magnetic Resonance Spectroscopy, Soil Pollution, Toxicity, Zinc Compounds, Dna Base, 113cd Magnetic Resonance Spectroscopy, Article, Binding Affinity, Carcinogenesis, Chemical Structure, Nonhuman, Protein Domain, Zinc Finger Motif, Arabidopsis Proteins, Base Pairing, Base Sequence, Cysteine, Dna Primers, Plant, Histidine, Models, Molecular, Biomolecular, Protein Conformation, Spectrophotometry, Ultraviolet, Transcription Factors, Chemistry, Genetics, Hydroxymethylglutaryl Coenzyme A Reductase Inhibitor, Placebo, Arterial Wall Thickness, Cardiovascular Disease, Cardiovascular Risk, Letter, Priority Journal, Prognosis, Risk Assessment,
Affiliations: *** IBB - CNR ***
Dipartimento di Scienze Ambientali, II University of Naples, via Vivaldi 43, 81100 Caserta, Italy
Istituto di Biostrutture e Bioimmagini - CNR, Via Mezzocannone 16, 80134 Napoli, Italy
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples Federico II, Via Mezzocannone 16, 80134 Napoli, Italy
Department of Internal Medicine, Cardiovascular Science and Immunology, Federico II University of Naples, via S. Pansini 5, 80131 Naples, Italy
References: Not available.
Zinc to Cadmium Replacement in the A. thaliana SUPERMAN Cys(2)His(2) Zinc Finger Induces Structural Rearrangements of Typical DNA Base Determinant Positions
Among heavy metals, whose toxicity cause a steadily increasing of environmental pollution, cadmium is of special concern due to its relatively high mobility in soils and potential toxicity at low concentrations. Given their ubiquitous role, zinc fingers domains have been proposed as mediators for the toxic and carcinogenic effects exerted by xenobiotic metals. To verify the structural effects of zinc replacement by cadmium in zinc fingers, we have determined the high resolution structure of the single Cys(2)His(2) zinc finger of the Arabidopsis thaliana SUPERMAN protein (SUP37) complexed to the cadmium ion by means of UV-vis and NMR techniques. SUP37 is able to bind Cd(II), though with a dissociation constant higher than that measured for Zn(II). Cd-SUP37 retains the beta beta alpha fold but experiences a global structural rearrangement affecting both the relative orientation of the secondary structure elements and the position of side chains involved in DNA recognition: among them Ser17 side chain, which we show to be essential for DNA binding, experiences the largest displacement. (C) 2011 Wiley Periodicals, Inc. Biopolymers 95: 801-810, 2011.
Zinc to Cadmium Replacement in the A. thaliana SUPERMAN Cys(2)His(2) Zinc Finger Induces Structural Rearrangements of Typical DNA Base Determinant Positions
Zinc to Cadmium Replacement in the A. thaliana SUPERMAN Cys(2)His(2) Zinc Finger Induces Structural Rearrangements of Typical DNA Base Determinant Positions