An assessment of the operating characteristics of Coulter Counter model S-Plus STKR(758 views) Salvati AM, d'Onofrio G, Berti P, Cappabianca MP, Marsili G, De Philippis C, Cossa L, Quarantelli M, Zini G, Mango G
Affiliations: Laboratorio di Biochimica Clinica, Istituto Superiori di Sanita, Viale Regina Elena 299, 00161 Roma, Italy
References: Not available.
An assessment of the operating characteristics of Coulter Counter model S-Plus STKR
Background and Methods. The operating performance of the Coulter Counter S Plus STKR was evaluated in two hospital laboratories in Rome and in Florence. Experimental design conformed to both the ICSH and NCCLS Standards for the evaluation of hematologic analyzers, and to the ECCLS guidelines for the multicenter evaluation of analyzers in clinical chemistry. Results and Conclusions. Cell counts in K3 EDTA were unchanged over 6 hours at room temperature and 72 hours at 4-degrees-C, while MCV, MPV and leukocyte differentials were far less stable. Carry over, precision and linearity met the manufacturer's specifications, while a satisfactory relative accuracy was demonstrated by determining reference values on an adult reference group and by comparing the instrument with the previous model S Plus IV D. The accuracy of the leukocyte differentials was evaluated by the microscope reference method, and our results seemed to validate the hypotheses that the STKR model counts: i) eosinophils, basophils and banded neutrophils among GR; ii) variant lymphocytes among LY, and iii) various abnormal cells among mononuclear cells. However, in spite of this statistical significance, some difficulties in correctly classifying the mononuclear population were demonstrated.
An assessment of the operating characteristics of Coulter Counter model S-Plus STKR
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